164,728 results
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Plasmid#207757PurposeDonor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
dCas9 plasmid
Plasmid#100091PurposedCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expressionDepositorTypeEmpty backboneUseCRISPRTags3XFlag-NLS-dCas9-NLSExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC25A48
Plasmid#131995PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A48 (SLC25A48 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
B-GRAM-H
Plasmid#211706PurposeExpression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H) in mammalian cellsDepositorInsertB-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseTagsddFP-B3ExpressionMammalianMutationChanged Glycine 187 to LeucinePromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG_IGF1R_EGFP
Plasmid#199628PurposeFRET donor for IGF1R sensorDepositorInsertIGF1R (IGF1R Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK1200
Plasmid#84219PurposeLentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherryDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICSL50010
Plasmid#50310DepositorInsertC terminal Myc tag (4x Myc)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE28
Plasmid#90370PurposeTFEB - Lysosomal biogenesis gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterTFEBAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-pre-mGRASP-mCerulean
Plasmid#34910PurposepreDepositorHas ServiceAAV5Insertpresynaptic mGRASP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Cx43-7
Plasmid#54055PurposeLocalization: Gap Junctions, Excitation: 487, Emission: 509DepositorInsertCx43
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSCV-opEBNA2-IRES-mCherry
Plasmid#220355PurposeExpresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression)DepositorInsertopEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
UseRetroviralTagsExpressionMammalianMutationcodon-optimised for expression in mammalian cellsPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHIS-wt7D12
Plasmid#125268PurposeExpresses wild type 7D12 anti-EGFR nanobodiesDepositorInsert7D12 nanobody
UseTagsHis-TagExpressionBacterialMutationPromoterTrcAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 - TRC mTagBFP2
Plasmid#191566PurposeExpresses an shRNA and mTagBFP2DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNAAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDCAF3-IS5
Plasmid#65220PurposeExpresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.DepositorInsertsndmA
ndmB
ndmC
ndmD
gst9
chlR
UseSynthetic BiologyTagsExpressionBacterialMutationChanged start codon from gtg to atg and Changed s…PromoterBBa_J23100Available sinceJan. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV7 mitoDendra2
Plasmid#157757PurposeExpression of the fluorescent protein Dendra2 targeted to mitochondriaDepositorInsertDendra2
UseTagsExpressionMammalianMutationMTS coxVIII +Dendra2PromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pComb8
Plasmid#63889PurposeFor phage display of antibody libraries by fusion to modified geneVIII.DepositorTypeEmpty backboneUsePhage displayTagsgeneVIIIExpressionBacterialMutationPromoterlacZAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-SARS2-B.1.617.2
Plasmid#172320Purposeexpressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.DepositorInsertSpike of B.1.617.2 strain (S SARS-CoV-2)
UseTagsExpressionMammalianMutationT19R, 156G, 157-158del, L452R, T478K, D614G, P681…PromoterCMV-FAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
FSW-tdTomato
Plasmid#197033PurposeExpress tdTomato from hSyn1 promoterDepositorInserttdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOGG001
Plasmid#113978PurposeLevel 0 module. nptII promoter for constitutive gene expressionDepositorInsertnptII promoter
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
LARRYv2-EGFP library 2
Pooled Library#233216PurposeBarcoding pooled libraryDepositorSpeciesSyntheticUseLentiviralAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLX317 PRDM14
Plasmid#193682PurposeConstitutive lentiviral expression of PRDM14DepositorInsertPRDM14 (PRDM14 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-A3-HA-ubiquitin
Plasmid#220145PurposeExpresses A3-Ub for E3 substrate identification by E-STUBDepositorInsertUbiquitin C (UBC Human)
UseTagsA3-HAExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
CDS12_mTurquoise2
Plasmid#136089PurposeFP for N-term fusion with a CTAGDepositorInsertCDS12_mTurquoise2(noStop)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMpGE013
Plasmid#108681PurposeAll-in-one genome editing vector in M. polymorpha (hygromycin resistant)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICSL86977OD
Plasmid#86180PurposeGG compatible CDs acceptor BASTADepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
I48 ELP
Plasmid#68937PurposeElastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residueDepositorInsertI48 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-2Aneo v2
Plasmid#80033PurposeUsed to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trappingDepositorTypeEmpty backboneUseAAV and Cre/Lox; A platform vector to construct t…TagsExpressionMutationPromoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC39A6_STOP
Plasmid#161319PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC39A6 (SLC39A6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUPD+Red-F
Plasmid#170874PurposePhytobrick - Red-F Luciferase CDSDepositorInsertRed-F
UseLuciferase and Synthetic BiologyTagsExpressionMutationp.S286Y Red mutantPromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Tensin1-C-14
Plasmid#54274PurposeLocalization: Focal Adhesions, Excitation: 487, Emission: 509DepositorInsertTensin1 (TNS1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits