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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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161,697 results

Showing: 11751 - 11800 of 161697 results

  2. PB-TET

    Plasmid
    #20909
    Purpose
    piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)
    Depositor
    Andras Nagy
    Article
    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi
    Insert
    Gateway Destination Vector
    Use
    piggybac
    Tags
    IRES-bGeo
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2009
    Availability
    Academic Institutions and Nonprofits only
    20909-plasmid-map-sequence-id-174009

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  3. TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

    Plasmid
    #216154
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216154-plasmid-map-sequence-id-437765

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  13. mEos3.2-Tubulin-C-18

    Plasmid
    #57484
    Purpose
    Localization: Microtubules, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Tubulin (TUBA1B Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57484-plasmid-map-sequence-id-140118

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  19. pCMV-HA (New MCS)

    Plasmid
    #32530
    Depositor
    Christopher A Walsh
    Type
    Empty backbone
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32530-plasmid-map-sequence-id-351565

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  24. pDual_dsCas9_Blast

    Plasmid
    #214681
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214681-plasmid-map-sequence-id-428811

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  30. CCR5-DuET

    Plasmid
    #213201
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR5 (CCR5 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213201-plasmid-map-sequence-id-430764

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  31. pAAV-CMV-FLEX-SaCas9-U6-sgDrd2

    Plasmid
    #159918
    Purpose
    Mutagenesis of Drd2
    Depositor
    Larry Zweifel
    Insert
    Drd2 (Drd2 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159918-plasmid-map-sequence-id-317332

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  38. Tet-pLKO-FOXM1-shRNA-81

    Plasmid
    #89497
    Purpose
    Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.
    Depositor
    Adam Karpf
    Insert
    FOXM1 (FOXM1 Human)
    Use
    Lentiviral and RNAi; Doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO
    Available since
    June 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    89497-plasmid-map-sequence-id-181253

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  39. pGreenFire1_E2F1RE

    Plasmid
    #112248
    Purpose
    expression of GFP and Luciferase under the control of E2F1 responsive element
    Depositor
    Simone Di Giovanni
    Insert
    E2F1RE (responsive element)
    Use
    Lentiviral
    Tags
    copGFP and luciferase
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    112248-plasmid-map-sequence-id-213659

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  41. hSyn1-eYFP (AAV2)

    Viral Prep
    #117382-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Article
    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:
    Promoter
    Syn
    Tags
    eYFP
    Available since
    Feb. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 11751 - 11800 of 161697 results