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Plasmid#207759PurposeDonor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPBpuro-EGFR(M721)
Plasmid#197379PurposeEncoding human ErbB1 mutant.DepositorInsertEGFR mutant (EGFR Human)
UseTagsExpressionMammalianMutationLysine 721 to MethioninePromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0471
Plasmid#142635PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKAT7 (KAT7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0470
Plasmid#142270PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKAT7 (KAT7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
KTK_002
Plasmid#180527PurposeEntry vector containing J23104 promoterDepositorInsertJ23104 promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT3928
Plasmid#113331Purposecontrol for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoterDepositorInsertsbeta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-EGFP
Plasmid#91975PurposeEGFP reporter cell line control (no miR-19 sites)DepositorInsertEGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRE4-Bpa
Plasmid#155342PurposePlasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.DepositorInserthumanized EBpaRS/BstYam
UseTagsExpressionMammalianMutationPromoterPGK/U6Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VSVG
Plasmid#35616DepositorInsertVSV G protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3-mArg1 promoter/enhancer -31/-3810
Plasmid#34571DepositorInsertArg1 promoter/enhancer -31/-3810 (Arg1 Mouse)
UseLuciferaseTagsFirefly LuciferaseExpressionMutationPromoterAvailable sinceJan. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
TFORF0707
Plasmid#143889PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNCOR1 (NCOR1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-mTurquoise
Plasmid#172376PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertmTurquoise
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-T-rnVAPA-1-219
Plasmid#13395DepositorInsertVAP-A (Vapa Rat)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX-T-rnVAPA-1-219
Plasmid#13395DepositorInsertVAP-A (Vapa Rat)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV5B-Flag-Smurf2 wt
Plasmid#11746DepositorInsertSMURF2 (SMURF2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 12, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV5B-Flag-Smurf2 wt
Plasmid#11746DepositorInsertSMURF2 (SMURF2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 12, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRZeroT
Plasmid#120276PurposeBifunctional vector for TA-cloning and blunt-end cloning of PCR-products using ZERO-backgound systemDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
PGK-AAVS1ZFNR
Plasmid#60915Purposeencodes zinc finger nuclease specific to AAVS1 locus (right)DepositorInsertAAVS1-ZFNR
UseTagsExpressionMutationPromoterPGKAvailable sinceFeb. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2) cloning backbone
Plasmid#61424PurposesgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-ack
Plasmid#62654PurposeArabinose inducible lambda red and anhydrotetracycline inducible sgRNA expressionDepositorInsertexo, beta, gam, sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-Histag-mCherry (527)
Plasmid#70719PurposeExpression vector for mCherry purificationDepositorInsertmCherry
UseTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF1
Plasmid#228775PurposeProtein expression plasmid for YTH domain of YTHDF1 in E.Coli.DepositorInsertYTH domain of YTHDF1 (YTHDF1 Human)
UseTags6*HisExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX458M-53BP1-DN1S
Plasmid#131045PurposePlasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.DepositorInsertSpCas9-53BP1-DN1S (TP53BP1 Human)
UseCRISPRTagsGFP and SpCas9ExpressionMammalianMutationOnly expressing residues 1231-1644 of complete 53…PromoterCBHAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
DsRed-rab11 WT
Plasmid#12679DepositorInsertRAB11A (Homo sapiens) (RAB11A Human)
UseTagsDsRed and HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
DsRed-rab11 WT
Plasmid#12679DepositorInsertRAB11A (Homo sapiens) (RAB11A Human)
UseTagsDsRed and HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEFCas9
Plasmid#87115PurposeSpCas9 expression AAV backbone plasmid under control of nEF promoterDepositorInsertnEF-Cas9
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-mCherry
Plasmid#114470PurposemCherry ControlDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertmCherry
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF3459
Plasmid#144935PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHMGB2 (HMGB2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-A3-HA-ubiquitin
Plasmid#220145PurposeExpresses A3-Ub for E3 substrate identification by E-STUBDepositorInsertUbiquitin C (UBC Human)
UseTagsA3-HAExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132B
Plasmid#69282PurposeGolden Gate entry vector; 2nd gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Snap-Omp25
Plasmid#69599PurposeSnap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondriaDepositorInsertSnap-Omp25
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF3286
Plasmid#144762PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR2F6 (NR2F6 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Nrf1-Myc pcDNA3.1(+)-C-Myc
Plasmid#169125Purposeexpression of proteinDepositorInserthNrf1 (NFE2L1 Human)
UseTagsExpressionMammalianMutationwtPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-BbTagBY (AAV9)
Viral Prep#45185-AAV9PurposeReady-to-use AAV9 particles produced from AAV-EF1a-BbTagBY (#45185). In addition to the viral particles, you will also receive purified AAV-EF1a-BbTagBY plasmid DNA. Brainbow construct encoding farnesylated TagBFP and EYFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsTagBFP, EYFP, or none.Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mRuby3-Zeo
Plasmid#167206PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with ZeocinDepositorTypeEmpty backboneUseCRISPRTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (AAV5)
Viral Prep#100054-AAV5PurposeReady-to-use AAV5 particles produced from pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (#100054). In addition to the viral particles, you will also receive purified pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 plasmid DNA. CAG-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmCherryAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 puro
Plasmid#104994PurposeThis 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCWX-UBI-hTert-PGK-BSD
Plasmid#114316PurposeConstitutive expression of the hTert proteinDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-Vac 14 Full Length
Plasmid#47418DepositorInsertVac14 (VAC14 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
ColorfulCell
Plasmid#62449PurposeMXS_chaining vector with 6 cassettesDepositorInsertsCMV::GTS-Citrine-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::NLS-TagBFPx3-bGHpA
CMV::MTS-AzamiGreen-bGHpA
CMV::iRFP670-SKL-bGHpA
CMV::ETS-mCherry-KDEL-bGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only