Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

173,387 results

Showing: 9521 - 9560 of 173387 results

  6. iOn-CAG∞RFP

    Plasmid
    #154014
    Purpose
    Vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase
    Depositor
    Jean Livet
    Article
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    mRFP1
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154014-plasmid-map-sequence-id-308093

    Enlarge

  21. G protein alpha-s(alpha3beta5/G226A/A366S)

    Plasmid
    #55797
    Purpose
    This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    alpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T, G226A, A366S i…
    Promoter
    CMV
    Available Since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55797-plasmid-map-sequence-id-112181

    Enlarge

  22. HTT delta-exon1

    Plasmid
    #162274
    Purpose
    Baculovirus and mammalian expression
    Depositor
    Cheryl Arrowsmith
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expresssion
    Tags
    10xHis-Flag
    Expression
    Mammalian
    Promoter
    CMV and P10
    Available Since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162274-plasmid-map-sequence-id-345745

    Enlarge

  24. pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid
    #119945
    Purpose
    Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18
    Insert
    Renilla luciferase
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Promoter
    TetCMV
    Available Since
    March 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    119945-plasmid-map-sequence-id-483465

    Enlarge

  29. EGFP-Vimentin-7

    Plasmid
    #56439
    Purpose
    Localization: Intermediate Filaments, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    56439-plasmid-map-sequence-id-323617

    Enlarge

  30. PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid
    #102890
    Purpose
    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102890-plasmid-map-sequence-id-198249

    Enlarge

  31. pAAVdual-CMV-eGFP

    Plasmid
    #230931
    Purpose
    pAAVdual plasmid is used to package AAV-CMV-eGFP viruses with the AAVdual system, which integrates the mini-pHelper-1.0 (providing E2A, E4orf6, and VA RNA) with an AAV expression cassette containing eGFP driven by the CMV promoter.
    Depositor
    Qizhao Wang
    Article
    Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2
    Insert
    eGFP
    Use
    AAV
    Promoter
    CMV
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230931-plasmid-map-sequence-id-458357

    Enlarge

  35. TFORF0508

    Plasmid
    #143407
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TSHZ2 (TSHZ2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    143407-plasmid-map-sequence-id-389032

    Enlarge

  37. pGPΩTp

    Plasmid
    #130660
    Purpose
    Insertional mutagenesis of GP transposon into chromosome of B. cenocepacia. Trimethoprim selection for plasmid.
    Depositor
    Miguel Valvano
    Article
    Flannagan et al Infect Immun. 2007 Apr;75(4):1679-89. Ep
    Insert
    Ω trimethoprim cassette
    Expression
    Bacterial
    Mutation
    Addition of Ω sites next to trimethoprim cassette…
    Promoter
    dhfr promoter
    Available Since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    130660-plasmid-map-sequence-id-258172

    Enlarge

  40. pSCMV-H2B-FT-Medium

    Plasmid
    #157670
    Purpose
    Retroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.
    Depositor
    Shangqin Guo
    Article
    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi
    Insert
    Histone H2B
    Use
    Retroviral
    Tags
    Medium-FT
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    157670-plasmid-map-sequence-id-308113

    Enlarge

Showing: 9521 - 9560 of 173387 results