We narrowed to 1,529 results for: Streptococcus

1. pX165-eSpCas9

   Plasmid

   #140564

   Purpose

   Expresses eSpCas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mutation

   K848A K1003A R1060A

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMJ824

   Plasmid

   #39314

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   GST and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRPaCas9

   Plasmid

   #111819

   Purpose

   Expresses Cas9 in T. brucei (2T1) cells

   Depositor

   David Horn

   Article

   Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMC-J6-dCas9-24XGP41-J9

   Plasmid

   #202006

   Purpose

   MoClo level 0 dCas9-24XGP41 coding sequence module

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Insert

   dCas9-24XGP41
   

   Use

   Synthetic Biology; Moclo cloning vector

   Tags

   GS linkers

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX165-HypaCas9

   Plasmid

   #140567

   Purpose

   Expresses HypaCas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mutation

   N692A M694A Q695A H698A

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT3TS-nCas9n-A71

   Plasmid

   #125644

   Purpose

   micro-injection of poly-adenylated nCas9n RNA

   Depositor

   Jason Berman

   Article

   Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

   Insert

   nCas9n
   

   Use

   Rna in vitro transcription vector

   Tags

   Expression

   Mutation

   Promoter

   T3

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Yeast_Target-AID3S

   Plasmid

   #188646

   Purpose

   Expresses Target-AID3S in yeasts

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Insert

   nCas9-AID2S-UGI
   

   Use

   Tags

   UGI

   Expression

   Yeast

   Mutation

   D10A for SpCas9

   Promoter

   pGal1

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. EC2_7_dCas9_HC_sgRNA

   Plasmid

   #163711

   Purpose

   High copy plasmid with dCas9 and sgRNA expression cassettes

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX165-evoCas9

   Plasmid

   #140569

   Purpose

   Expresses evoCas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mutation

   M495V Y515N K526E R661Q

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEPOR0SP0013

    Plasmid

    #117521

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-p

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-p (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(-)-PM-Cpalm-HA-SrtA (pKS37)

    Plasmid

    #50031

    Purpose

    Expression of S. pyogenes SrtA at the plasma membrane in mammalian cells

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

    Insert

    PM-SrtA(strep)
    

    Use

    Tags

    C-HA

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. PV247

    Plasmid

    #132338

    Purpose

    CasA constitutive expression cassette for Zea mays

    Depositor

    Joshua Young

    Article

    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

    Insert

    CasA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Codon optimize for expression and stability in Ze…

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMK17-02

    Plasmid

    #99604

    Purpose

    Plasmid containing parBp(mut)-m(sf)gfp fusion

    Depositor

    Jan-Willem Veening, Morten Kjos

    Article

    van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

    Insert

    parBp(mut)
    

    Use

    Synthetic Biology

    Tags

    m(sf)gfp

    Expression

    Mutation

    internal parS-site mutated

    Promoter

    PczcD2

    Available since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICSL1P3_AtU626Ter26

    Plasmid

    #191771

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICSL11197

    Plasmid

    #191784

    Purpose

    SpCas9 expression in plants

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    AtUbi10::Cas9 +13 introns-T-E9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTricHis-ILY-prepore

    Plasmid

    #160519

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-prepore
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    I104C, G244C

    Promoter

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTricHis-ILY-WT-Y336W

    Plasmid

    #160515

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-WT-Y336W
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Y336W

    Promoter

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. EC2_1_dCas9_sgRNA

    Plasmid

    #163710

    Purpose

    Yeast low copy plasmid with dCas9 and sgRNA expression cassette

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82)

    Plasmid

    #50032

    Purpose

    Galactose-inducible expression of ER-luminal S. pyogenes SrtA in yeast

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

    Insert

    HA-SrtA(strep)
    

    Use

    Tags

    HA-HDEL

    Expression

    Yeast

    Mutation

    Promoter

    pGAL

    Available since

    Jan. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEPOR0SP0016

    Plasmid

    #117526

    Purpose

    Level0 Golden Gate part: CDS, Sp-eCas9 1.0

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Sp-eCas9 1.0 (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    K810A, K1003A, R1060A

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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