We narrowed to 952 results for: Streptococcus

1. pBBK13 pCAS-Tyr-[gRNA: 3=XII-2] (SplitKanR, AmpR)

   Plasmid

   #178997

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBBK10 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitURA3,AmpR)

   Plasmid

   #178994

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBBK09 pCAS-Tyr-[gRNA: BsaI GFP dropout] (BsmBI-flanked URA3, AmpR)

   Plasmid

   #178993

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = URA3

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)

   Plasmid

   #178992

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBBK07 pCAS-Tyr-[gRNA: BsaI GFP dropout] (LEU2,AmpR)

   Plasmid

   #178991

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = LEU2

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)

   Plasmid

   #178990

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26

   Plasmid

   #149346

   Purpose

   Mammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNA

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Cbc

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCbh-SpyCas9-2A-puro-pA_U6-BbsI

   Plasmid

   #149347

   Purpose

   Mammalian expression vector for SpyCas9 and puromycin resistance

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   SpyCas9
   

   Use

   CRISPR

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)

   Plasmid

   #149350

   Purpose

   Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   SpyCas9
   

   Use

   CRISPR

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3TS-Hypa-nCas9n-A69

    Plasmid

    #125645

    Purpose

    micro-injection of poly-adenylated Hypa-nCas9n RNA

    Depositor

    Jason Berman

    Article

    Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

    Insert

    Hypa-nCas9n
    

    Use

    Rna in vitro transcription vector

    Tags

    Expression

    Mutation

    N692A, M694A, Q695A, H698A from wild-type Cas9

    Promoter

    T3

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCF704

    Plasmid

    #121658

    Purpose

    U6-sgRNA EFS-ProCas9-Flavi-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    Cas9(C)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCF712

    Plasmid

    #121663

    Purpose

    U6-sgRNA EF1a-ProCas9-Flavi-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    Cas9(C)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196082

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196080

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAc-sgRNA-Cas9

    Plasmid

    #49330

    Purpose

    Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

    Depositor

    Ji-Long Liu

    Article

    Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

    Inserts

    Cas9
    dU6-sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Insect

    Mutation

    Human codon optimised

    Promoter

    Actin-5c and Drosophila U6

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCV-Cas9-2A-GFP-sgRNA

    Plasmid

    #124889

    Purpose

    Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

    Depositor

    Harvey Lodish

    Article

    Li et al Haematologica. 2016 Jun;101(6):e216-9. d

    Insert

    Cas9
    

    Use

    Retroviral

    Tags

    2A-GFP

    Expression

    Mutation

    Promoter

    MSCV

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. gRNA NIA TLS2

    Plasmid

    #196981

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS2 tags
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pU6-26, pU6-29

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOSIP-KH-RBS2-dCas9

    Plasmid

    #182740

    Purpose

    Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

    Depositor

    David Bikard

    Article

    Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

    Insert

    dCas9
    

    Use

    CRISPR; Integrative vector

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Pb-TRE3G-SpCas9-IRES-blast

    Plasmid

    #195506

    Purpose

    Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

    Depositor

    Joanna Wysocka

    Article

    Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRCT

    Plasmid

    #60621

    Purpose

    Plasmid encoding iCas9, tracrRNA and crRNAs

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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