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Showing: 7231 - 7260 of 160936 results

  4. SpyTag003-MBP

    Plasmid
    #133450
    Purpose
    Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm
    Depositor
    Mark Howarth
    Article
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Insert
    SpyTag003-MBP
    Use
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Peptide tag added to N-terminus of MBP via a GSGE…
    Promoter
    T7
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133450-plasmid-map-sequence-id-267039

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  5. pCAG-CBE4max-SpRYc-P2A-EGFP

    Plasmid
    #208336
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc
    Depositor
    Pranam Chatterjee
    Article
    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:
    Insert
    human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    Sc++ (1-1119), SpRY (1111-1368), D10A
    Promoter
    CAG
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    208336-plasmid-map-sequence-id-439176

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  7. miReporter-CAG

    Plasmid
    #82478
    Purpose
    microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.
    Depositor
    Pierre Neveu
    Article
    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi
    Insert
    H2B-mCherry and H2B-Citrine. HygroR
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    82478-plasmid-map-sequence-id-174379

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  14. ITGAV-bio-His

    Plasmid
    #51919
    Purpose
    Expresses full-length Integrin alpha-V precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ITGAV (ITGAV Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51919-plasmid-map-sequence-id-79896

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  19. pAAV-nEF Con/Fon DREADD Gq-mCherry

    Plasmid
    #183532
    Purpose
    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp
    Depositor
    Karl Deisseroth
    Insert
    DREADD Gq-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    None
    Promoter
    nEF
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    183532-plasmid-map-sequence-id-362037

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  20. EphA2-YFP

    Plasmid
    #108852
    Purpose
    Encodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker
    Depositor
    Kalina Hristova
    Article
    Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.
    Insert
    EPHA2 (EPHA2 Human)
    Use
    Tags
    Labeled with eYFP on the C-Terminus via a 15 amin…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    108852-plasmid-map-sequence-id-208239

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  22. PB-TO-ASCL1-DLX2

    Plasmid
    #182307
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    Ascl1 (Ascl1 Mouse)
    Dlx2 (Dlx2 Mouse)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    None and T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and pTRE3G-bidirectional
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182307-plasmid-map-sequence-id-359517

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  27. mScarletI3-6DG5-MAPPER

    Plasmid
    #222421
    Purpose
    To express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)
    Depositor
    Stephen Royle
    Article
    Downie et al bioRxiv 2024.05.31.596797
    Insert
    MAPPER
    Use
    Tags
    mScarletI3-6DG5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222421-plasmid-map-sequence-id-439142

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Showing: 7231 - 7260 of 160936 results