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pEF1a-BirA-V5-neo

Plasmid

#100548

Purpose

Expresses C-terminal V5-tagged BirA biotin ligase

Depositor

Jian Xu

Publication

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

C-terminal V5-tagged BirA

Tags

V5, His

Expression

Mammalian

Promoter

Human EF1a

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pSFFV_mNG2(11)1-10

Plasmid

#82610

Purpose

Lentiviral vector of constitutive expression of mNeonGreen2(1-10)

Depositor

Bo Huang

Publication

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNeonGreen2(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

K128M, S142T, R150M, G172V and K213M

Promoter

SFFV

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEGFP-C1-hApg5

Plasmid

#22952

Depositor

Noboru Mizushima

Publication

Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

Insert

Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)

Tags

EGFP

Expression

Mammalian

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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PIK3CA-WT

Plasmid

#16643

Depositor

Bert Vogelstein

Publication

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PIK3CA (PIK3CA Human)

Tags

Flag

Expression

Mammalian

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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FUW-SOKM

Plasmid

#20325

Purpose

Lentiviral plasmid expressing mouse Sox2, Oct4, Klf4 and cMyc for iPS cell generation

Depositor

Rudolf Jaenisch

Publication

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-P2A-Oct4-T2A-Klf4-E2A-cMyc (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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lenti_dCas9-KRAB-MeCP2

Plasmid

#122205

Purpose

Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

Depositor

Andrea Califano

Publication

Tan et al bioRxiv 2021.06.28.449297

Insert

dCas9-KRAB-MeCP2

Use

Lentiviral

Tags

KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 (D10A, H840A)

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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tet-pLKO-sgRNA-puro

Plasmid

#104321

Purpose

3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

Depositor

Nathanael Gray

Publication

Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1/TO

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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Human Beta-catenin GFP

Plasmid

#71367

Purpose

mammalian expression of human Beta-catenin GFP

Depositor

Alpha Yap

Publication

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Beta-catenin (CTNNB1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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dCas9-KRAB

Plasmid

#110820

Purpose

Conventional dCas9 repressor-dCas9-KRAB

Depositor

Alejandro Chavez, George Church

Publication

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-KRAB

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pCMVHA E2F1

Plasmid

#24225

Purpose

Mammalian expression of human E2F1 with HA tag

Depositor

Kristian Helin

Publication

Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

Insert

E2F1 (E2F1 Human)

Tags

HA

Expression

Mammalian

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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pcDNA4-Vps34-Flag

Plasmid

#24398

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Vps34 (PIK3C3 Human)

Tags

3xFLAG

Expression

Mammalian

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pEcCas

Plasmid

#73227

Purpose

Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

Depositor

Sheng Yang

Publication

Li et al Acta Biochim Biophys Sin (Shanghai). 202

Inserts

Cas9
sgRNA

Use

CRISPR; Red recombinase

Expression

Bacterial

Promoter

native promoter

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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PPRE X3-TK-luc

Plasmid

#1015

Purpose

PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

Depositor

Bruce Spiegelman

Publication

Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

Insert

PPRE

Use

Luciferase

Expression

Mammalian

Available since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

99 Articles

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pEF1a-FB-dCas9-puro

Plasmid

#100547

Purpose

Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein

Depositor

Jian Xu

Publication

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
puromycin

Use

CRISPR

Tags

FLAG, Biotin-acceptor-site (Avitag)

Expression

Mammalian

Promoter

Human EF1a

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pET His6 GST TEV LIC cloning vector (1G)

Plasmid

#29655

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, GST, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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1477 pcDNA3 flag HA Akt1

Plasmid

#9021

Depositor

William Sellers

Publication

Hsieh et al Nucleic Acids Res. 2004 . 32(3):893-901

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Akt1 (AKT1 Human)

Tags

HA and flag

Expression

Mammalian

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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Toronto KnockOut version 3 library (TKOv3)

Pooled library

#90294

Purpose

Optimized library offering improved accuracy, efficiency, and scalability for CRISPR screens compared to TKOv1

Depositor

Jason Moffat

Publication

Hart et al G3 (Bethesda). 2017 Aug 7;7(8):2719-2727

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

62 Articles
pcDNA3.1-2xFLAG-SREBP-1c

Plasmid

#26802

Depositor

Timothy Osborne

Publication

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SREBP-1c (SREBF1 Human)

Tags

2XFLAG

Expression

Mammalian

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pBPGUw

Plasmid

#17575

Depositor

Gerald Rubin

Publication

Pfeiffer et al Tools for neuroanatomy and neurogenetics

Insert

GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

43 Articles

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HDAC7 Flag

Plasmid

#13824

Purpose

Mammalian expression of human histone deacetylase 7 with flag tag

Depositor

Eric Verdin

Publication

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC7 (HDAC7 Human)

Tags

flag

Expression

Mammalian

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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