167,825 results

1. pNCS-mRuby3

   Plasmid

   #74234

   Purpose

   Constitutive expression of mRuby3 in bacteria

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   mRuby3
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 SIRT7_H187Y

   Plasmid

   #53151

   Purpose

   expression vector for catalytically inactive human Sirtuin7

   Depositor

   Katrin Chua

   Article

   Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

   Insert

   Sirt7 (SIRT7 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H187Y, catalytic inactive

   Promoter

   CMV

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-p53

   Plasmid

   #12091

   Depositor

   Tyler Jacks

   Article

   Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

   Insert

   p53 (TP53 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   72R variant of p53

   Available Since

   July 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. ML14

   Bacterial Strain

   #61911

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. tyrA gene deleted.

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

   Bacterial Resistance

   None

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

5. FLAG-FBXL17

   Plasmid

   #236188

   Purpose

   Expression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.

   Depositor

   Michele Pagano

   Article

   Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

   Insert

   FLAG-FBXL17 (FBXL17 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. 2MLS-HyperNova/pcDNA3

   Plasmid

   #221457

   Purpose

   Expressing HyperNova in mitochondria

   Depositor

   Kiwamu Takemoto

   Article

   Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

   Insert

   HyperNova fused with a tandem repeat of the mitochondria localization signal from cytochrome c oxidase subunit VIII
   

   Expression

   Mammalian

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Zeo-mStayGold

   Plasmid

   #227252

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mStayGold cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. EK0397 3FLAG-ADAR1-p150-HIS (pEAQ)

   Plasmid

   #191172

   Purpose

   Human ADAR1, p150 isoform in plant-expressable format.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   3FLAG-ADAR1-p150-HIS (plant) (ADAR Human)
   

   Tags

   3FLAG and HIS

   Expression

   Plant

   Mutation

   WT

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-Cas9-T2A-GFP

   Plasmid

   #63155

   Purpose

   Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Insert

   Cas9-T2A-GFP
   

   Use

   CRISPR; Tol2 middle entry vector for gateway

   Available Since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-OTp-GCaMP6s

    Plasmid

    #192945

    Purpose

    To drive GCaMP6s under the control of mouse oxytocin promoter

    Depositor

    Kazunari Miyamichi

    Article

    Yaguchi et al PLoS One. 2023 May 10;18(5):e0285589. do

    Insert

    GCaMP6s
    

    Use

    AAV

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. hOT-GFP

    Plasmid

    #67848

    Purpose

    mammalian expression of human oxytocin receptor with GFP tag

    Depositor

    Christian Gruber

    Article

    Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

    Insert

    oxytocin receptor (OXTR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pDNR-mCherry-T2A-3xPuroR-Frame 0

    Plasmid

    #206991

    Purpose

    Donor plasmid for Frame 0 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

    Depositor

    Alejandro Chavez

    Article

    Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    mCherry

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-FLAG-KLF5

    Plasmid

    #40918

    Depositor

    Jin-Tang Dong

    Article

    Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

    Insert

    Kruppel-like factor 5 (KLF5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. phOct4-EGFP

    Plasmid

    #38776

    Depositor

    Wei Cui

    Article

    Gerrard et al Stem Cells. 2005;23(1):124-33.

    Insert

    Oct-4 promoter (POU5F1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    human OCT4

    Available Since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-PGK-EGFP-ALDH1A2-Neo

    Plasmid

    #189743

    Purpose

    The construct was used to express GFP tagged human ALDH1A2 in mammalian cells for the analysis of the subcellular location of this protein.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A2 (ALDH1A2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET51b(+)_SNAP-tag2

    Plasmid

    #226512

    Purpose

    Bacterial cell expression of SNAP-tag2

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    SNAP-tag2
    

    Tags

    His-tag (10x) and Strep-Tag II,

    Expression

    Bacterial

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCh-Rab7A

    Plasmid

    #61804

    Purpose

    Human Rab7A fluorescently tagged with mCh on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. zWnt5b

    Plasmid

    #21282

    Depositor

    Randall Moon

    Article

    Stoick-Cooper et al Development. 2007 Feb . 134(3):479-89.

    Insert

    Wnt5b (wnt5b Zebrafish)
    

    Available Since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-hfYFP

    Plasmid

    #186523

    Purpose

    Hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Hyperfolder YFP
    

    Expression

    Mammalian

    Mutation

    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

    Plasmid

    #209082

    Purpose

    NFIB and SOX9 mediated astrocye induction

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
    mKate2
    

    Expression

    Mammalian

    Promoter

    TRE4G

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDAC439

    Plasmid

    #195237

    Purpose

    Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
    

    Expression

    Mammalian

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pN7CAST

    Plasmid

    #190661

    Purpose

    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

    Plasmid

    #61628

    Purpose

    Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter

    Depositor

    Dolf Weijers

    Article

    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

    Inserts

    ntdTomato
    n2xEGFP
    

    Expression

    Plant

    Mutation

    2 copies of EGFP with N-terminal SV40 NLS

    Promoter

    Auxin-dependent DR5 promoter containing 9 reverse…

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA-mC/EBPb

    Plasmid

    #49198

    Purpose

    expression of mouse C/EBPbeta in mammalian cells

    Depositor

    Jed Friedman

    Insert

    C/EBPbeta (Cebpb Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (AAV9)

    Viral Prep

    #192552-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (#192552). In addition to the viral particles, you will also receive purified pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA plasmid DNA. CAG-driven expression of nuclear localized tdTomato. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Guoping Feng

    Promoter

    CAG

    Tags

    tdTomato

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA3 Flag Pu.1

    Plasmid

    #66974

    Purpose

    Expression plasmid for mouse Pu.1; adds Flag tag to the N-terminus of protein

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Spi1 (Spi1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3.1/cyto-iAspSnFR

    Plasmid

    #201394

    Purpose

    CMV overexpression of cyto-iAspSnFR in mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Hellweg et al Cell Chem Biol. 2024 May 20:S2451-9456(2

    Insert

    cyto-iAspSnFR
    

    Expression

    Mammalian

    Mutation

    gltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…

    Promoter

    CMV

    Available Since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pflareG-psd95-exon18

    Plasmid

    #90266

    Purpose

    Minigene reporter for alternative splicing analysis

    Depositor

    Douglas Black

    Article

    Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

    Insert

    Psd95-exon18 (Dlg4 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCS2-mutFAKSPARK2

    Plasmid

    #195264

    Purpose

    express Tyr to Phe mutant FAKSPARK2

    Depositor

    Xiaokun Shu

    Article

    Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

    Insert

    Tyr to Phe mutant FAKSPARK2 sensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. mARGAna (cassette 2)

    Plasmid

    #191342

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 2)
    

    Expression

    Mammalian

    Promoter

    TRE3GV

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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31. TFORF2258

    Plasmid

    #142927

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX11 (SOX11 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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32. mRNA-CleanCap-mNeonGreen

    Plasmid

    #188248

    Purpose

    Template plasmid to transcribe mRNA encoding mNeonGreen

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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33. TRE-UBC-hCD55-T2A-GFP

    Plasmid

    #205459

    Purpose

    lentiviral plasmid for expression of human CD55

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD55 (CD55 Human)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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34. mEmerald-TGNP-N-10

    Plasmid

    #54279

    Purpose

    Localization: Golgi Complex, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    TGNP (TGOLN2 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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35. pBAD-HisD-miniGFP2

    Plasmid

    #188897

    Purpose

    Express 6xHis-Tag miniGFP2 in E.coli

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP2
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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36. pLJM1-FLAG-metap2

    Plasmid

    #100510

    Purpose

    Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    METAP2 (METAP2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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37. pT3TS-spRYCas9-ABE-UMax

    Plasmid

    #222139

    Purpose

    Near PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    TadA-nSpRYCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    bipartite NLS

    Promoter

    T3

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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38. pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE (AAV8)

    Viral Prep

    #130991-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE (#130991). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE plasmid DNA. CamKIIa-driven, soma-targeted ChRmine-mScarlet expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Promoter

    CaMKII

    Tags

    mScarlet

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

39. pRK5-HA-Ubiquitin-K29

    Plasmid

    #22903

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K29 only, all other lysines mutated to arginines

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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40. pLSB-HYG

    Plasmid

    #166699

    Purpose

    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.

    Depositor

    Robin Allshire

    Article

    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

    Inserts

    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    

    Expression

    Yeast

    Promoter

    adh15 promoter and tRNA promoter

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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