164,868 results

1. pCDNA5/FRT_CMV_NES-Kinprola_PKC-mEGFP

   Plasmid

   #233357

   Purpose

   CMV driven expression of the PKC activity recorder Kinprola_PKC in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

   Insert

   NES-Kinprola_PKC-mEGFP
   

   Use

   Tags

   NES and mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYY166 pcDNA3.4-His-MBP-hAID

   Plasmid

   #231942

   Purpose

   Expression of His-MBP-AID fusion protein in mammalian cells

   Depositor

   Fei-Long Meng

   Article

   Wang et al Cell. 2023 May 11;186(10):2193-2207.e19.

   Insert

   AID (AICDA Human)
   

   Use

   Tags

   His-MBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCV006-ermg

   Plasmid

   #232824

   Purpose

   Random transposon mutagenesis vector for Bacteroidales species - erythromycin

   Depositor

   Athanasios Typas

   Insert

   ermG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-Dyskerin

   Plasmid

   #207577

   Purpose

   N-terminal mCherry-tag using SalI/EcoRI

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   generated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFSKm4-ATF1

   Plasmid

   #221133

   Purpose

   Formaldehyde sensor based on the frmR repressor, which expresses yeast alcohol acetyl transferase (ATF1) upon detection

   Depositor

   André Studart

   Article

   Dutto et al Adv Mater. 2024 Dec 10:e2412555. doi: 10

   Insert

   ATF1 (ATF1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pm4

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW-HA-hRb-delta-CDK-T373D-S608D-S612D-puro

   Plasmid

   #212675

   Purpose

   Express tagged hRb phosphosite mutant

   Depositor

   Yumi Konagaya, Tobias Meyer

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   Rb (RB1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   15 CDK phospho-sites are mutated to alanines, and…

   Promoter

   TRE

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Anti-Shank1 [N22/21R] (Recombinant Antibody)

   Antibody

   #209594-rAb

   Purpose

   Anti-Shank1 (Rat) recombinant mouse monoclonal antibody

   Depositor

   James Trimmer

   Article

   Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

   Recommended Applications

   Western Blot

   Reactivity

   Human, Mouse, and Rat

   Source Species

   Mouse

   Isotype

   IgG2a

   Trial Size

   Available to purchase

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

8. pAWO-SUMO-3C_14M_zTDP2

   Plasmid

   #200512

   Purpose

   Expression of zebrafish TDP2 recombinant protein

   Depositor

   Antony Oliver

   Insert

   TDP2 (tdp2b Zebrafish)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   L136N, A139S, C239T, K240R, K309Q, K314L, T315G, …

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2629

   Plasmid

   #142966

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   INSM2 (INSM2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD649-HAsp-NCR1-COMP5AP-AviTag-9xHis

    Plasmid

    #157253

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    NCR1 (NCR1 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Sender_plasmid2

    Plasmid

    #140693

    Purpose

    Encodes inducible expression of RhlI (C4-HSL quorum sensing synthase)

    Depositor

    Matthew Bennett

    Article

    Alnahhas et al ACS Synth Biol. 2019 Sep 20;8(9):2051-20

    Insert

    RhlI (rhlI )
    

    Use

    Tags

    ssrA degradation tag (ASV)

    Expression

    Bacterial

    Mutation

    Promoter

    engineered lac promoter (IPTG inducible)

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIRES2-EGFP-SHP2WT

    Plasmid

    #12283

    Depositor

    Anton Bennett

    Article

    Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

    Insert

    SHP-2WT (PTPN11 Human)
    

    Use

    Tags

    Myc (not canonical: KLISEEDL)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-DIO-PPO-Venus (AAV8)

    Viral Prep

    #139505-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-DIO-PPO-Venus (#139505). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-PPO-Venus plasmid DNA. EF1a-driven, Cre-dependent expression of Venus-tagged parapinopsin for spatiotemporal control of inhibitory GPCR signaling cascades. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Promoter

    EF1a

    Tags

    Venus (Cre-dependent)

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

14. pEQ276

    Plasmid

    #83945

    Purpose

    express human cytomegalovirus IE1 and IE2 genes in eukaryotic cells

    Depositor

    Adam Geballe

    Article

    Biegalke et al Virology. 1991 Jul;183(1):381-5.

    Insert

    IE1 and IE2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pmirGLO-3'UTR mouse Il13 5xBoxB

    Plasmid

    #207128

    Purpose

    Tethered luciferase reporter for mouse Il13 BoxB

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    Interleukin 13 (Il13) 3'UTR (Il13 Mouse)
    

    Use

    Luciferase

    Tags

    5xBoxB

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. GALT-SEP-mRuby3

    Plasmid

    #200938

    Purpose

    trans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Inserts

    GalT (B4GALT1 Human)
    SEP-mRuby3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-hNox4

    Plasmid

    #69352

    Purpose

    expresses human Nox4 in mammalian cells

    Depositor

    Karl-Heinz Krause

    Article

    Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

    Insert

    hNOX4 (NOX4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-45b-mCherry-NPM1

    Plasmid

    #194546

    Purpose

    Bacterial Expression of 6xHis mCherry-NPM1 Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    NPM1 (Npm1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHN922

    Plasmid

    #178975

    Purpose

    Expression of GST-His8-GFP-nanobody

    Depositor

    Kazuhiro Abe

    Insert

    GST-His8-GFP-nanobody
    

    Use

    Affinity Reagent/ Antibody

    Tags

    GST, His8

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126706

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pIR-CMV-SCN1A-Variant-1-IRES-mScarlet

    Plasmid

    #162278

    Purpose

    Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

    Depositor

    Al George

    Article

    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

    Insert

    SCN1A Variant 1, stabilized (SCN1A Human)
    

    Use

    Tags

    IRES-mScarlet

    Expression

    Mammalian

    Mutation

    IVS intron inserted after bp2946. Silent codon c…

    Promoter

    CMV

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUCmini-iCAP-AAV1.X1

    Plasmid

    #209780

    Purpose

    non-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

    Insert

    Synthetic construct isolate AAV1-X1 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCW57.1-FOXM1c

    Plasmid

    #68810

    Purpose

    Doxycycline inducible lentiviral vector for FOXM1c expression in mammalian cells

    Depositor

    Adam Karpf

    Article

    Barger et al Oncotarget. 2015 Jul 16.

    Insert

    FOXM1c (FOXM1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. YPet-pBAD

    Plasmid

    #54860

    Purpose

    Localization: Protein Expression Vector , Excitation: 513, Emission: 527

    Depositor

    Patrick Daugherty, Michael Davidson

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

    Type

    Empty backbone

    Use

    Tags

    6xHis and YPet

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

25. pLV-REPORT (PGK) NanoLuc

    Plasmid

    #208576

    Purpose

    Empty NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    NanoLuciferase
    d2eGFP
    Empty response element
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    PGK and miniP

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGP-CMV-jGCaMP7s

    Plasmid

    #104463

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7s
    

    Use

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. BirA pLEMO

    Plasmid

    #119818

    Purpose

    Express endogenous biotin ligase in E.coli

    Depositor

    Alexander Gabibov

    Article

    Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

    Insert

    Biotin ligase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Rhamnose promoter

    Available since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Flag-mPIAS1

    Plasmid

    #15206

    Depositor

    Ke Shuai

    Article

    Liu et al Proc Natl Acad Sci U S A. 1998 Sep 1. 95

    Insert

    PIAS1 (Pias1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-CAG-GFP (AAV2 trial size)

    Viral Prep

    #37825-AAV2.T

    Purpose

    Ready-to-use AAV2 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    GFP

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

30. pcDNA3.3_SOX2

    Plasmid

    #26817

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-SOX2-3'UTR (SOX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216246

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgAAVS1.5
    

    Use

    CRISPR; Human safe harbor locus (a avs1) site-spe…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA-R-diLACCO1

    Plasmid

    #208028

    Purpose

    Control biosensor for intracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-diLACCO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGDP2 MCR-1

    Plasmid

    #118404

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    MCR-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLac

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PB-UBC-GCAMP6f-PGK-neo

    Plasmid

    #160049

    Purpose

    expression of GCaMP6f in a piggybac plasmid

    Depositor

    Xiaojun Lian

    Insert

    GCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pIS1_Opt_Renilla

    Plasmid

    #210571

    Purpose

    Expresses Optimal Renilla luciferase

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Optimal Renilla
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HSV TK; T7

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TACR3-Tango

    Plasmid

    #66516

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    TACR3 (TACR3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40

    Plasmid

    #100852

    Purpose

    AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRGECO1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDUAL SRBI (GFP)

    Plasmid

    #86979

    Purpose

    Lentiviral expression construct encoding SR-B1 and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    SCARB1 (SCARB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV/hPGK

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRSetB-KanR-LiLac

    Plasmid

    #184569

    Purpose

    Fluorescent reporter for lactate (with lifetime and intensity response); for bacterial or in vitro expression

    Depositor

    Gary Yellen

    Article

    Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

    Insert

    LiLac fluorescent sensor of lactate
    

    Use

    Tags

    His7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. lenti-EGFR-L858R-tmpknot-epegRNA

    Plasmid

    #214092

    Purpose

    Lentiviral vector expressing epegRNA to induce EGFR L858R mutation

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    EGFR L858R epegRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge