164,909 results

1. pBS_12x-601_MA1

   Plasmid

   #114358

   Purpose

   12x Widom 601 nucleosome sequence

   Depositor

   Scot Wolfe

   Article

   Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

   Insert

   12X601-MA1
   

   Use

   Unspecified

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-Puro-CLDN18

   Plasmid

   #69466

   Purpose

   Expresses CLDN18

   Depositor

   Axel Hillmer

   Article

   Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

   Insert

   CLDN18 (CLDN18 Human)
   

   Use

   Retroviral

   Tags

   HA TAG

   Expression

   Mutation

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1-P-CAG-GFP

   Plasmid

   #80491

   Purpose

   donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   CAG-GFP
   

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. NID1-bio-His

   Plasmid

   #52068

   Purpose

   Expresses full-length Nidogen-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   NID1 (NID1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E-csGBP

   Plasmid

   #108891

   Purpose

   Multisite gateway vector for 3' tagging with a conditionally stable nanobody against GFP

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   csGBP
   

   Use

   Multisite gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-DBNF-mCherry

   Plasmid

   #187195

   Purpose

   To express the mouse BDNF tagged with the FP mCherry

   Depositor

   Pantelis Tsoulfas

   Insert

   BDNF
   

   Use

   Lentiviral

   Tags

   fluorescent protein mCherry

   Expression

   Mammalian

   Mutation

   The codons have been optimized for better express…

   Promoter

   CMV

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUW-tetO-Pu.1

   Plasmid

   #139839

   Purpose

   doxycycline-inducible expression of mouse Pu.1 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Pu.1 (Spi1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-mCMV

   Available since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-Nsp12

   Plasmid

   #165117

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp12 (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. GAL4UAS-Luciferase reporter

   Plasmid

   #64125

   Purpose

   Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   GAL4UAS
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   GAL4UAS

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid

    #50831

    Purpose

    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

    Depositor

    Peter Schultz

    Article

    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Inserts

    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    

    Use

    Baculoviral

    Tags

    His, Myc-6xHis, and WPRE

    Expression

    Insect

    Mutation

    Y39TAG and able to charge various unnatural amino…

    Promoter

    CAG, CMV, and U6 and H1

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-Wnt7A

    Plasmid

    #35914

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt7A (WNT7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5xUAS-Flag-NPM1wt

    Plasmid

    #131822

    Purpose

    4-hydroxytamoxifen inducible vector expressing Flag-NPM1wildtype (human) in mammalian cells through transduction

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-PP7-2xmCherry-CAAX

    Plasmid

    #74925

    Purpose

    Expresses PP7-2xmCherry-CAAX

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    PP7-2xmCherry-CAAX
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-Puro_hCOL4A4

    Plasmid

    #198099

    Purpose

    lentiviral expression of hCOL4A4 in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A4 (COL4A4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Tol2-ISceI-E1b:EGFP;cryaa:mCherry-ISceI-Tol2

    Plasmid

    #193851

    Purpose

    E1b promoter for transgenic enhancer assays in zebrafish

    Depositor

    Junsu Kang

    Article

    Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. cyto-iATPSnFR1.0

    Plasmid

    #102550

    Purpose

    cytoplasmic ATP sensor for mammalian cells

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 TEV (full-length)

    Plasmid

    #64276

    Purpose

    Expresses tobacco etch virus protease (TEV)

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Tobacco etch virus (TEV) protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCNICK

    Plasmid

    #84653

    Purpose

    Genome editing for Lactobacillus casei Lc2W

    Depositor

    Sheng Yang

    Article

    Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

    Inserts

    Cas9 nickase
    sgRNA
    homology arms of LC2W_2179
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKrox24(2xD-E)dTomato

    Plasmid

    #200110

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    modified EGR1 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    2 copies of D-E element, no other promoter elemen…

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF1898

    Plasmid

    #144116

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PATZ1 (PATZ1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

    Plasmid

    #64324

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. p006-Banana-Late

    Plasmid

    #112251

    Purpose

    ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    ATF1 under osmY promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    osmY

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAS9counter

    Plasmid

    #107192

    Purpose

    Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

    Depositor

    Steve Salipante

    Article

    Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

    Inserts

    Cas9
    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    prab17

    Available since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCK302

    Plasmid

    #87768

    Purpose

    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

    Inserts

    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    

    Use

    Synthetic Biology

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD rhamnose-inducible promoter from E. coli …

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

    Plasmid

    #214885

    Purpose

    Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-21a_3xNLS_SpCas9_protein_expression

    Plasmid

    #114365

    Purpose

    Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

    Insert

    3xNLS_SpCas9
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EYFP-hCALML3

    Plasmid

    #47605

    Depositor

    Emanuel Strehler

    Article

    Rogers et al J Biol Chem. 2001 Apr 13;276(15):12182-9

    Insert

    CALML3 (CALML3 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA3.1 EYFP H148Q/I152L

    Plasmid

    #25872

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q/I152L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with H148Q and I152L m…

    Promoter

    Available since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJMP2846

    Plasmid

    #160676

    Purpose

    Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

    Depositor

    Jason Peters

    Article

    Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Epsin1 deltaUIM-ECFP

    Plasmid

    #20973

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Epsin1 deltaUIM (Epn1 Rat)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2009

    Availability

    Academic Institutions and Nonprofits only