167,468 results

1. Ornithine transcarbamylase (OTC)

   Plasmid

   #71877

   Purpose

   For mitochondrial unfolded protein response research

   Depositor

   Nicholas Hoogenraad

   Article

   Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

   Insert

   OTC (Otc Rat)
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLBS-mScarlet

   Plasmid

   #129337

   Purpose

   cytosol marker, lentivirus

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al bioRxiv 2021.10.08.463131

   Insert

   mScarlet (Addgene 85042)
   

   Use

   Lentiviral

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFR-PATagRFP

   Plasmid

   #31950

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

   Insert

   PATagRFP
   

   Tags

   EGF receptor (EGFR)

   Expression

   Mammalian

   Mutation

   R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

   Promoter

   CMV

   Available Since

   Oct. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. Rtn4a-GFP

   Plasmid

   #61807

   Purpose

   Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

   Depositor

   Gia Voeltz

   Article

   Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

   Insert

   Rtn4a (RTN4 Human)
   

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Rtn4a Stop Codon removed

   Promoter

   CMV

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV8)

   Viral Prep

   #105545-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   HI

   Tags

   EGFP

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pUC18

   Plasmid

   #50004

   Purpose

   pUC cloning vector

   Depositor

   Joachim Messing

   Article

   Norrander et al Gene. 1983 Dec;26(1):101-6.

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5xATF6-GL3

   Plasmid

   #11976

   Purpose

   Reporter gene with five ATF6 sites that is highly sensitive to ER stress

   Depositor

   Ron Prywes

   Article

   Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

   Insert

   5xATF6 binding site
   

   Tags

   Luc and c-fos promoter

   Expression

   Mammalian

   Available Since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

   Plasmid

   #61593

   Purpose

   A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   HA, NLS, and OLLAS tag

   Expression

   Mammalian

   Mutation

   K175R and K736R (deUb mutations)

   Available Since

   Feb. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ1371-sgSTAT3-1

   Plasmid

   #121425

   Purpose

   sgSTAT3-1 sequence: GTCAGGATAGAGATAGACCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Stanley Qi, Ovijit Chaudhuri

   Article

   Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

   Insert

   sgSTAT3-1
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLminP_Luc2P_RE55

    Plasmid

    #90400

    Purpose

    C-REL - NFkB gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    CREL

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-Hb9-RFP

    Plasmid

    #37081

    Depositor

    Fred Gage

    Article

    Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

    Insert

    Hb9 RFP
    

    Use

    Lentiviral

    Promoter

    Hb9

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE (AAV5)

    Viral Prep

    #202199-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE (#202199). In addition to the viral particles, you will also receive purified pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE plasmid DNA. Synapsin-driven, Cre-dependent expression of bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized opsin PdCO. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Promoter

    Syn

    Tags

    EGFP

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

13. pYC-C

    Plasmid

    #207114

    Purpose

    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

    Depositor

    Yifan Cheng

    Article

    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGB 35S:Renilla:Tnos (GB0109)

    Plasmid

    #68215

    Purpose

    Transcriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Renilla
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTXB1-CfaC-Gyra

    Plasmid

    #122950

    Purpose

    Bacterial expression of C-intein

    Depositor

    Thomas Muir

    Article

    Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

    Insert

    CfaC
    

    Expression

    Bacterial

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. p35S_GFP-GUS

    Plasmid

    #167122

    Purpose

    Plant binary expression vector containing mGFP5-GUS fusion reporter under CaMV 35S promoter.

    Depositor

    Olivier Voinnet, Arturo Marí-Ordóñez

    Article

    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

    Insert

    GFP-GUS
    

    Tags

    mGFP5, GUS

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti F9-TetR-EGFP-IRES-PuroR

    Plasmid

    #117049

    Purpose

    lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Insert

    F9-TetR-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    F9 promoter

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-IP-mVenus-TOSI

    Plasmid

    #172491

    Purpose

    mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    Pdcd4 (Pdcd4 Mouse)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Promoter

    MLV LTR

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Liu Mouse CRISPR Knockout Library in lentiCRISPRv2-blast

    Pooled Library

    #1000000173

    Depositor

    Xiaole Shirley Liu

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

20. Cas12f-GE ver4.1

    Plasmid

    #176544

    Purpose

    Expresses Cas12f-GE in mammalian cells

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

    Inserts

    Cas12f-GE ver4.1
    Cas12f-GE ver4.1
    

    Expression

    Mammalian

    Promoter

    U6 and chicken β-actin

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGEX-6P-1_WT RAD23B

    Plasmid

    #201457

    Purpose

    Expresses human RAD23B with an N-terminal GST tag in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    tac promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pEP4 E02S CK2M EN2L

    Plasmid

    #20924

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc (MYC, SOX2, KLF4, NANOG, POU5F1, LIN28A Human)
    

    Use

    Episomal/ebna

    Available Since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCR2.1 Clover-LMNA Donor

    Plasmid

    #122508

    Purpose

    Homology repair template for in frame (first exon) clover knock-in of human LMNA gene

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    Homology Repair Template for Human LMNA (N-Terminal Clover Tag) (LMNA Human)
    

    Use

    Homology repair template plasmid (donor plasmid)

    Tags

    Clover

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCMV-CRY2-mCherry

    Plasmid

    #58368

    Purpose

    Mamlian codon optimized CRY2

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (CRY2 Mustard Weed)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. qTAG-C-mScarlet-EFS-Blast

    Plasmid

    #227260

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET15bpATnp

    Plasmid

    #121137

    Purpose

    This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

    Depositor

    Keji Zhao

    Article

    Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

    Insert

    Protein A-Tnp
    

    Tags

    his

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-TRE-DIO-ChR2-TdTomato

    Plasmid

    #166600

    Purpose

    Encodes Cre-dependent ChR2-TdTomato under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    ChR2-TdTomato
    

    Use

    AAV

    Mutation

    H134R

    Promoter

    tetracycline response element

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pSMAL-CellTag-V2

    Pooled Library

    #115644

    Purpose

    Viral barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics

    Depositor

    Samantha Morris

    Article

    Biddy et al Nature. 2018 Dec;564(7735):219-224. doi:

    Has Service

    Concentrated Lentiviral Prep

    Expression

    Mammalian

    Species

    Synthetic

    Use

    Lentiviral

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

29. pJW1518

    Plasmid

    #62388

    Purpose

    pFA6a -mCherry-BirA::HIS5

    Depositor

    Jonathan Weissman

    Article

    Williams et al Science. 2014 Nov 7;346(6210):748-51. do

    Insert

    mCherry-BirA
    

    Use

    Yeast c-terminal tagging vector

    Tags

    mCherry-BirA

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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30. pJFRC81-10XUAS-IVS-Syn21-GFP-p10

    Plasmid

    #36432

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Proc Natl Acad Sci U S A. 2012 Apr 9.

    Insert

    GFP
    

    Expression

    Insect

    Available Since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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