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167,642 results

Showing: 10561 - 10600 of 167642 results

  2. qTAG-N-Solo-mStayGold-ACTB

    Plasmid
    #227326
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a mStayGold Tag (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227326-plasmid-map-sequence-id-452114

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  3. qTAG-N-Puro-mStayGold-TUBA1B

    Plasmid
    #227321
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227321-plasmid-map-sequence-id-452094

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  5. FFA2-DuET

    Plasmid
    #213238
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    FFA2 (FFAR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213238-plasmid-map-sequence-id-430796

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  8. pLV-Kif5b-T92N-Myc

    Plasmid
    #186615
    Purpose
    Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B Rigor mutant (T92N) fused to Myc tag
    Depositor
    Gia Voeltz
    Article
    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6
    Insert
    Kinesin Heavy Chain isoform 5b Rigor mutant (T92N) (KIF5B Human)
    Use
    Lentiviral
    Tags
    Myc tag
    Expression
    Mammalian
    Mutation
    T92N
    Promoter
    CMV
    Available Since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    186615-plasmid-map-sequence-id-371213

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  10. pET28a-XPO5-His6

    Plasmid
    #171562
    Purpose
    Encodes human XPO5 protein
    Depositor
    Yan Zeng
    Insert
    XPO5 (XPO5 Human)
    Expression
    Bacterial
    Available Since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171562-plasmid-map-sequence-id-337920

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  16. pAAV-mDlx-ChR2-mCherry-Fishell-3 (AAV Retrograde)

    Viral Prep
    #83898-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-mDlx-ChR2-mCherry-Fishell-3 (#83898). In addition to the viral particles, you will also receive purified pAAV-mDlx-ChR2-mCherry-Fishell-3 plasmid DNA. mDlx-driven expression of ChR2 fused to mCherry. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    mDlx
    Tags
    mCherry
    Available Since
    March 2, 2020
    Availability
    Academic Institutions and Nonprofits only

  21. gRNA_Cloning Vector BbsI

    Plasmid
    #128433
    Purpose
    Embty backbone vector for expression of gRNA for spCas9 genome editing or epigenome editing
    Depositor
    Izuho Hatada
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    July 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    128433-plasmid-map-sequence-id-251645

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  24. HCA2-Tango

    Plasmid
    #66396
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    HCA2 (HCAR2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66396-plasmid-map-sequence-id-372748

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  26. pDS24

    Plasmid
    #58952
    Purpose
    Encodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.
    Depositor
    Thomas Fox
    Article
    Steele et al Proc Natl Acad Sci U S A. 1996 May 28;93
    Insert
    Mitochondrially coded Arg8 protein
    Mutation
    Coding sequence synthetic in S. cerevisiae mitoch…
    Promoter
    n/a
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  31. pDB5318

    Plasmid
    #204828
    Purpose
    An integrating plasmid containing both the inducible enotetS promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.
    Depositor
    Li-Lin Du
    Article
    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404
    Inserts
    tetR
    mECitrine
    Expression
    Yeast
    Promoter
    CMV promoter and enotetS promoter
    Available Since
    Aug. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    204828-plasmid-map-sequence-id-405721

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  34. pPing

    Plasmid
    #47100
    Purpose
    Expresses the Ping open reading frame 1 (ORF1) and transposase from rice to allow mPing movement. The vector contains ORF1, transposase, mPing element and hph for hygromycin selection.
    Depositor
    Wayne Parrott
    Article
    Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d
    Inserts
    Ping cDNA
    mPing
    hygromycin resistance gene
    Use
    Plant expression
    Tags
    GFP
    Expression
    Yeast
    Promoter
    CaMV35s and StUbi3
    Available Since
    Sept. 16, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47100-plasmid-map-sequence-id-67166

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Showing: 10561 - 10600 of 167642 results