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Showing: 10801 - 10840 of 160936 results

  2. Flag-APEX3-C Cloning Vector

    Plasmid
    #220615
    Purpose
    To express a protein of interest fused to the C-terminus of Flag-APEX3 for proximity labeling or TEM (unpublished)
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    Flag-APEX3 fusion
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    220615-plasmid-map-sequence-id-435941

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  3. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV Retrograde)

    Viral Prep
    #154867-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ulrik Gether
    Article
    Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03
    Promoter
    Human Synapsin-1
    Tags
    mCherry (Flp-dependent)
    Available since
    Oct. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  4. pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt

    Plasmid
    #175181
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Has Service
    AAV1
    Insert
    pAAV hSyn FLEX iGluSnFR3 v857.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI Anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    175181-plasmid-map-sequence-id-345963

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  7. smHA multi-frame tag

    Plasmid
    #128611
    Purpose
    a spaghetti monster with 10x HA epitopes repeat upstream of the HIV frameshift sequence which is followed by FLAG epitopes in the 0ORF are separated from one another by SunTag epitopes in the -1ORF.
    Depositor
    Tim Stasevich
    Article
    Lyon et al Mol Cell. 2019 Jul 11;75(1):172-183.e9.
    Insert
    smHA MF tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    128611-plasmid-map-sequence-id-251144

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  9. pET28a-SnoopCatcher

    Plasmid
    #72322
    Purpose
    Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a-SnoopCatcher
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    changed G842T and D848G of S. pneumoniae adhesin …
    Promoter
    T7
    Available since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72322-plasmid-map-sequence-id-137253

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  10. mCherry-Ykt6

    Plasmid
    #209888
    Purpose
    Expresses mCherry tagged Ykt6 in mammalian cells
    Depositor
    Geert van den Bogaart
    Insert
    Ykt6
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    209888-plasmid-map-sequence-id-413665

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  11. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Article
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Use
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    53261-plasmid-map-sequence-id-261354

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  19. p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2

    Plasmid
    #227775
    Purpose
    Expression of beta actin driven BFP with multiple cloning site at 3' end. Also contains heat-shock activated mCherry reporter and CreERT2.
    Depositor
    Bushra Raj
    Article
    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi
    Inserts
    BFP-MCS
    mCherry-t2A-CreERT2
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    Olactb and hsp70l
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    227775-plasmid-map-sequence-id-455547

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  25. pHLmMBP-8

    Plasmid
    #72347
    Purpose
    Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72347-plasmid-map-sequence-id-133755

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  26. mRuby-N1

    Plasmid
    #54581
    Purpose
    Localization: N1 Cloning Vector, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mRuby
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54581-plasmid-map-sequence-id-393318

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  32. BK Dunlop

    Plasmid
    #25466
    Depositor
    Peter Howley
    Insert
    human papovavirus BK Dunlop
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 26, 2011
    Availability
    Academic Institutions and Nonprofits only

  38. pMCB320

    Plasmid
    #89359
    Purpose
    3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites
    Depositor
    Michael Bassik
    Article
    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103
    Insert
    puromycin resistance, mCherry
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    89359-plasmid-map-sequence-id-176457

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  40. GCGR-Tango

    Plasmid
    #66291
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GCGR (GCGR Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 10801 - 10840 of 160936 results