167,318 results

1. pBABE HA Chk2 KD, dominant negative chk2

   Plasmid

   #41902

   Depositor

   Stephen Elledge

   Article

   Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

   Insert

   Chk2 (CHEK2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D347A

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA

   Plasmid

   #107313

   Purpose

   Encoding aCamkII-mCherry-Cre

   Depositor

   Hui Yang

   Article

   Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

   Insert

   aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDJSVT87

   Plasmid

   #109302

   Purpose

   Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tag

   Depositor

   Daniel Slade

   Article

   Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

   Type

   Empty backbone

   Tags

   OmpA 1-27, 6His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. NW9

   Plasmid

   #133442

   Purpose

   Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW9
   

   Expression

   Bacterial

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJM60 FLAG-RAB35 S22N

   Plasmid

   #107106

   Purpose

   Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

   Depositor

   Charles Sawyers

   Article

   Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

   Insert

   RAB35 (RAB35 Human)
   

   Use

   Lentiviral

   Tags

   FLAG tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDestTol2CG2--3.5ubb:plin2-tdtomato

   Plasmid

   #172534

   Purpose

   Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker

   Depositor

   Richard White

   Article

   Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

   Insert

   -3.5ubb:plin2-tdtomato
   

   Mutation

   S80R, A81S, E144D, I148F and R415H mutations obse…

   Promoter

   zebrafish ubb

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-VSVG

   Plasmid

   #35616

   Depositor

   Patrick Salmon, Arthur Nienhuis

   Insert

   VSV G protein
   

   Expression

   Mammalian

   Available Since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-CAG-flex-GFP-4x6T

   Plasmid

   #196418

   Purpose

   Cre-dependent AAV expression of GFP preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   GFP
   

   Use

   AAV and Cre/Lox; Astrocyte-selective

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHS2002

   Plasmid

   #239832

   Purpose

   Engineered OrufIscB (NovaIscB) omegaRNA mammalian expression

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

   Insert

   NovaIscB omegaRNA scaffold
   

   Expression

   Mammalian

   Mutation

   Replacement of A12-A57 with GAAA in omegaRNA scaf…

   Promoter

   human U6

   Available Since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Syn_CapChR2_mScarlet

    Plasmid

    #188039

    Purpose

    Expresses CapChR2-mScarlet in mammalian neurons

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2-mScarlet
    

    Use

    AAV

    Tags

    mScarlet

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    hSyn

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSIN4-EF2-ABCG2-IRES-Neo

    Plasmid

    #25983

    Depositor

    Ren-he Xu

    Article

    Zeng et al Stem Cells. 2009 Oct . 27(10):2435-45.

    Insert

    ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)
    

    Use

    Lentiviral

    Tags

    EF1-apha promoter and IRES-Neo

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEff

    Plasmid

    #97423

    Purpose

    Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

    Depositor

    Nikolai Ravin

    Article

    Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-Tight-Puro-KrasG12D2B

    Plasmid

    #139786

    Purpose

    Lentiviral tet-inducible expression of KrasG12D (mouse)

    Depositor

    Joan Massague

    Article

    Su et al Nature. 2020 Jan;577(7791):566-571. doi:

    Insert

    KrasG12D (Kras Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    G12D

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_ 2xFLAG-2xSTREP_KEAP1

    Plasmid

    #159128

    Purpose

    expresses KEAP1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    KEAP1 (KEAP1 Human)
    

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGWG_RUBYseed

    Plasmid

    #225989

    Purpose

    To clone any gene of interest using gateway recombination cloning and the vector expresses seed-specific RUBY

    Depositor

    Imran Siddiqi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Oct. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pLV-dCas9-p300-P2A-PuroR

    Plasmid

    #83889

    Purpose

    Lentiviral Sp dCas9-p300-P2A-PuroR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    pac from Streptomyces alboniger
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.hSyn.CaMPARI.WPRE.SV40 (AAV1)

    Viral Prep

    #100832-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.hSyn.CaMPARI.WPRE.SV40 (#100832). In addition to the viral particles, you will also receive purified pAAV.hSyn.CaMPARI.WPRE.SV40 plasmid DNA. Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger, Eric Schreiter

    Article

    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

    Promoter

    Syn

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-T7-TadCBEd-SpCas9(LWKYQS)-P2A-EGFP (BKS1014)

    Plasmid

    #223127

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(LWKYQS)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/LWKYQS)=D1135L, …

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.hSynap.iAChSnFR

    Plasmid

    #137950

    Purpose

    Expresses iAChSnFR under hSynapsin promoter

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Has Service

    AAV1

    Insert

    iAChSnFR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSynanpsin

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. PGKp-GFP-Empty

    Plasmid

    #174175

    Purpose

    Control lentiviral vector expressing GFP under control of the human PGK promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. EGFP-IC2-FL

    Plasmid

    #51409

    Purpose

    expression of EGFP-tagged full-length dynein intermediate chain IC2C in mammalian cells

    Depositor

    Trina Schroer

    Article

    King et al Mol Biol Cell. 2003 Dec;14(12):5089-97.

    Insert

    dynein intermediate chain IC2C (Dync1i2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCAGEN

    Plasmid

    #11160

    Purpose

    Mammalian expression vector for cloning and expressing your gene under the CAG promoter

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    modified multiple cloning sites
    

    Expression

    Mammalian

    Available Since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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23. pFRT_TO_eGFP_TIA1

    Plasmid

    #106094

    Purpose

    gene expression of EGFP-TIA1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    TIA1 (TIA1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pDONR221_SLC25A28

    Plasmid

    #132016

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A28 (SLC25A28 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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25. pMG046 pETARA-CK1α-His

    Plasmid

    #154074

    Purpose

    Expresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Tags

    GST and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. mCherry-Parkin

    Plasmid

    #23956

    Purpose

    Mammalian expression of human Park2 fused to mCherry

    Depositor

    Richard Youle

    Article

    Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

    Insert

    Park2 (PRKN Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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27. Cyto-V5-APEX2

    Plasmid

    #170572

    Purpose

    Expresses APEX2 in cytosol

    Depositor

    James Olzmann

    Article

    Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

    Insert

    APEX2
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCXLE-dCas9VP192-T2A-EGFP-shP53

    Plasmid

    #69535

    Purpose

    Episomal plasmid encoding dCas9VP192 and p53 shRNA

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    dCas9-dCas9VP192-GFP-shp53
    

    Use

    CRISPR

    Tags

    EGFP

    Promoter

    CAG

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. pBX_RPS2_smGFP_V5

    Plasmid

    #236088

    Purpose

    Constitutively encodes RPS2-smGFP-V5 (sfGFP-based spaghetti monster fluorescent protein embedding V5 epitope tags), for targeting the 40S subunit.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    RPS2-smGFP-V5 (RPS2 Synthetic, Human)
    

    Expression

    Mammalian

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCMV-Neo-Bam p53 R273H

    Plasmid

    #16439

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R273H (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R273H

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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