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hCas9-VPR

Plasmid

#68497

Purpose

nuclease competent SP-Cas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

SP-Cas9-VPR

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pFUGW SpCas9

Plasmid

#131506

Purpose

lentiviral expression of SpCas9 under hUBC promotor

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

SpCas9

Use

Lentiviral

Expression

Mammalian

Promoter

hUBC

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pFLAG-4EHP

Plasmid

#17342

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

4EHP (EIF4E2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only
pOlyA(E69A)

Plasmid

#132392

Purpose

Mutant version of OlyA that senses sphingomyelin without or with cholesterol

Depositor

Arun Radhakrishnan

Article

Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

Insert

Ostreolysin A point mutant (E69A)

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: C62S, E69A, C94S, S151C

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2

Plasmid

#86593

Purpose

Expresses eGFP-tagged human Akt2(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2 (AKT2 Human)

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCIP-AMPKa1_WT

Plasmid

#79010

Purpose

For mammalian expression of WT AMPK alpha1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

AMPK alpha 1 (PRKAA1 Human)

Expression

Mammalian

Promoter

pCAGGs

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-scFvGCN4sfGFP-VP64-GB1

Plasmid

#141417

Purpose

Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation

Depositor

Izuho Hatada

Article

Morita et al Int J Mol Sci. 2020 Feb 25;21(5). pii: i

Insert

scFvGCN4sfGFP-VP64-GB1

Tags

HA and sfGFP

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRU294

Plasmid

#167689

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-MITF

Plasmid

#64888

Purpose

Retroviral expression of murine MITF

Depositor

Ivana de la Serna

Article

de la Serna et al J Biol Chem. 2006 Jul 21;281(29):20233-4

Insert

MITF (Mitf Mouse)

Use

Retroviral

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) ZKSCAN1 MCS Exon Vector

Plasmid

#69901

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-eBFP2-NLS-pA

Plasmid

#183800

Purpose

AAV vector to express nuclear localized eBFP2 from CMV promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

eBFP2-NLS

Use

AAV

Tags

NLS

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pHyg-yeGFP-His6

Plasmid

#212781

Purpose

C-terminal PCR-based tagging with yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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mOPN-PB0

Plasmid

#106449

Purpose

Bacterial expression of murine osteopontin

Depositor

Georg Weber

Article

Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

Insert

osteopontin (Spp1 Mouse)

Use

Retroviral

Mutation

WT

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMIIS638

Plasmid

#238210

Purpose

Expresses native SpTasH TIGR locus. CmR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

SpTasH

Expression

Bacterial

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pMIIS644

Plasmid

#238209

Purpose

Expresses native FpTasA TIGR locus. CmR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

FpTasA

Expression

Bacterial

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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Dox-SOScat

Plasmid

#207329

Purpose

PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)

Depositor

Jared Toettcher

Article

Underhill et al Development. 2023 Aug 15;150(16):dev2016

Insert

son of sevenless homolog 2 variant, catalytic domain

Tags

myristoylation tag - BFP

Expression

Bacterial

Mutation

range: 593 to 1078

Promoter

Tet Response Element

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-mTagBFP-T2A-TetOn3G

Plasmid

#179888

Purpose

Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

TetOn3G

Use

Lentiviral

Tags

mTagBFP

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS HSPA6

Plasmid

#19540

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 6 (HSPA6 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA6

Plasmid

#19486

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 6 (HSPA6 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pMX229

Plasmid

#23002

Depositor

Linda Wordeman

Article

Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

Insert

Kif18A (KIF18A Human)

Tags

6His and EGFP

Expression

Mammalian

Available Since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pET-DUET-1-mRhubarb719-HO

Plasmid

#141200

Purpose

Expresses mRhubarb719 fluorescent protein and heme oxygenase

Depositor

Elad Firnberg

Article

Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

Inserts

mRhubarb719
heme oxygenase

Tags

6x-His

Expression

Bacterial

Mutation

R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

Promoter

T7

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/cmycDrosha

Plasmid

#10828

Depositor

Thomas Tuschl

Article

Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

Insert

Drosha (DROSHA Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA-UL

Plasmid

#108551

Purpose

Bacterial Target-AID UGI-LVA vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

dCas9-PmCDA1-UGI-LVA

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

lambda OR

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pMP11

Plasmid

#215559

Purpose

pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Inserts

Cas9
gRNA targeting pBR322 origin of replication

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23105 and tet

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-Gal4-vp16

Plasmid

#171075

Purpose

Expresses Gal4-vp16 fusion protein in bacteria

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

Gal4 DBD (NEWENTRY Budding Yeast)

Tags

His and VP16 AD (413-490)

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g10

Plasmid

#80793

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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TCARZ-CMV-Cherry-PAmin-GF3-R4A22

Plasmid

#203770

Purpose

For production of template RNA for use in PRINT, mCherry under CMV promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for mCherry

Use

Ivt template

Promoter

CMV

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-BE1

Plasmid

#73019

Purpose

Expresses BE1 with C-terminal NLS in mammalian cells

Depositor

David Liu

Article

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE1

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-Ataxin3Q84

Plasmid

#22124

Depositor

Henry Paulson

Article

Chai et al J Neurosci. 1999 Dec 1. 19(23):10338-47.

Insert

Ataxin 3 (ATXN3 Human)

Tags

myc

Expression

Mammalian

Available Since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only
CAV1-mEGFP

Plasmid

#27704

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

caveolin-1 (CAV1 Canis lupus familiaris)

Tags

monomeric EGFP

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

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Eef1a-1955/-1>nls::Cas9::nls

Plasmid

#59987

Purpose

Eef1a (EF1alpha) promoter driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Eef1a (EF1alpha) -1955/-1

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pRAN334

Plasmid

#120814

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)

Depositor

Craig Ellermeier, David S. Weiss

Article

Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

Insert

cyan fluorescent protein, codon-optimized for low GC organisms

Expression

Bacterial

Promoter

Ptet

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS1

Plasmid

#196977

Purpose

Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS1 tag

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCIP-AMPKa1_KD

Plasmid

#79011

Purpose

For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

AMPK alpha 1 (PRKAA1 Human)

Expression

Mammalian

Mutation

K47R

Promoter

pCAGGs

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE EF1α dCas9-VP64

Plasmid

#50918

Purpose

Constitutive dCas9-VP64 lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and VP64

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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mEGFP-RhoA-C1

Plasmid

#29674

Depositor

Ryohei Yasuda

Article

Murakoshi et al Nature. 2011 Apr 7;472(7341):100-4. doi:

Insert

RhoA (RHOA Human)

Tags

mEGFP

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
px459 VQR

Plasmid

#101715

Purpose

sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VQR (D1135V, R1335Q and T1337R)

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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double cut NH-donor

Plasmid

#83576

Purpose

eGFP reporter donor containing double SA cutting sites for NHEJ knock-in

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

eGFP

Use

Nhej donor

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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BAC-GWS1B

Plasmid

#239581

Purpose

Single copy expression vector for GWS1B Rubisco

Depositor

Matthew D. Shoulders

Article

McDonald et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

GWS1B Rubisco (6X his)

Tags

6X histag

Expression

Bacterial

Mutation

Wild-type

Promoter

T7, tac

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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