161,255 results

1. Tet-pLKO-FOXM1-shRNA-81

   Plasmid

   #89497

   Purpose

   Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.

   Depositor

   Adam Karpf

   Insert

   FOXM1 (FOXM1 Human)
   

   Use

   Lentiviral and RNAi; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO

   Available since

   June 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-TPH2-Cre

   Plasmid

   #189616

   Purpose

   Expresses Cre recombinase under the control of the tryptophan hydroxylase promoter

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   Cre Recombinase
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tph2

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. YIplac211-SEC7-msCFPx6

   Plasmid

   #105268

   Purpose

   Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-msCFPx6.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Sec7 portion (SEC7 Budding Yeast)
   

   Use

   Tags

   msCFPx6

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV10/3xflag-Clock

   Plasmid

   #47334

   Purpose

   full length clock in pCMV10 with 3x flag at the N-terminus

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   clock
   

   Use

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. FBE/SBE-Luc

   Plasmid

   #16525

   Depositor

   Bert Vogelstein

   Article

   Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

   Insert

   FBE/SBE
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pOZ-N-FH KIAA0157

   Plasmid

   #27499

   Depositor

   Roger Greenberg

   Article

   Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

   Insert

   KIAA0157 (ABRAXAS2 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pSH124-ssr

   Plasmid

   #198025

   Purpose

   Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   T0 terminator - Ap/Cb resistance cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ApR promoter

   Available since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-iCre

   Plasmid

   #89573

   Purpose

   Expresses iCre under pCAG

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   iCre
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV3Tag8li-hHP1BP3-HA

   Plasmid

   #165468

   Purpose

   Human HP1BP3 mammalian expression vector with C-terminal HA tag.

   Depositor

   Martin Dorf

   Article

   Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

   Insert

   Heterochromatin protein 1 binding protein 3 (HP1BP3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 His-NLRP1 codon-optimized

    Plasmid

    #166833

    Purpose

    Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tags

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG/ATP10D-HA

    Plasmid

    #209217

    Purpose

    Mammalian expression of ATP10D

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP10D (ATP10D Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF0832

    Plasmid

    #141725

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GTF2F1 (GTF2F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-PERK(LD)-EGFP

    Plasmid

    #225680

    Purpose

    Used to generate stable cell lines that express PERK(LD)-EGFP. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.

    Depositor

    Likun Wang

    Article

    Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lamin A (G465D)-mRFP

    Plasmid

    #124271

    Purpose

    Lamin A mutant (G465D), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.

    Depositor

    Eric Schirmer

    Insert

    LMNA (G465D) (LMNA Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    LMNA (G465D)

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-MAP3K5 (WT)

    Plasmid

    #47104

    Purpose

    Expresses wild type human MAP3K5 in mammalian cells

    Depositor

    Yardena Samuels

    Insert

    MAP3K5 (MAP3K5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    CMV

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQTEV-PTD004

    Plasmid

    #34798

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    GTP-binding protein PTD004 (OLA1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG/ATP9A(E195Q)-HA

    Plasmid

    #209244

    Purpose

    Mammalian expression of ATP9A mutant E195Q

    Depositor

    Hye-Won Shin

    Article

    Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

    Insert

    ATP9A (ATP9A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E195Q

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. V2-STING

    Plasmid

    #124265

    Purpose

    N-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

    Depositor

    Eric Schirmer

    Insert

    TMEM173 (STING1 Human)
    

    Use

    Tags

    Venus2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. B-GRAM-H

    Plasmid

    #211706

    Purpose

    Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H) in mammalian cells

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Tags

    ddFP-B3

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Leucine

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiPGK Hygro DEST ERKKTRmCerulean3

    Plasmid

    #90229

    Purpose

    Lentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    ERK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-Ef1a DIO-rsChRmine-oScarlet-WPRE

    Plasmid

    #183528

    Purpose

    Optogenetics

    Depositor

    Karl Deisseroth

    Article

    Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

    Insert

    rsChRmine-oScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    I146M/G174S

    Promoter

    Ef1a

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. plenti-UbcP-HA-CRBN-pGK-HYG

    Plasmid

    #107378

    Purpose

    Lentiviral expression of HA-CRBN

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    UbcP

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV5B-ALK4

    Plasmid

    #11756

    Depositor

    Jeff Wrana

    Article

    Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

    Insert

    ALK4 (ACVR1B Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

24. pAAV-pCALM1-EYFP-WPRE-bGHpA

    Plasmid

    #213971

    Purpose

    AAV expression of EYFP from pCALM1 promoter

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    pCALM1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mouse E-cadherin GFP (423)

    Plasmid

    #67937

    Purpose

    Mammalian expression of E-cadherin-GFP

    Depositor

    Alpha Yap

    Article

    Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

    Insert

    E-cadherin (Cdh1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MSCV-HA tagged-Mouse Mdm2 wt-IRES-GFP

    Plasmid

    #180491

    Purpose

    Role of Mdm2 splicing variants in tumorigenesis

    Depositor

    Martine Roussel

    Article

    Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

    Insert

    mouse Mdm2 full length (wt)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. EGFP-P2A-APEX2-ChBP

    Plasmid

    #108879

    Purpose

    Expression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    EGFP-P2A-APEX2-ChBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 5aa SunTag VP64 EVD

    Plasmid

    #115483

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MAC_C_TYRO3

    Plasmid

    #187793

    Purpose

    MAC-tagged gene expression of human TYRO3

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    TYRO3 (TYRO3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLentiCas9-T2A-GFP

    Plasmid

    #78548

    Purpose

    Cas9 protein with GFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with Cas9

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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