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Showing: 101 - 150 of 464 results

  10. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid
    #214690
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214690-plasmid-map-sequence-id-428775

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  11. pDual_dsCas9_Blast_hs_TP73

    Plasmid
    #214691
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214691-plasmid-map-sequence-id-428772

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  17. pXPR_213

    Plasmid
    #133456
    Purpose
    H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    Control guide
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133456-plasmid-map-sequence-id-261338

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  18. pDBEcR/Bla

    Plasmid
    #201556
    Purpose
    Plasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.
    Depositor
    Randy Poon
    Article
    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754
    Insert
    DBEcR
    Use
    Unspecified; Sleeping beauty transposon
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    201556-plasmid-map-sequence-id-400918

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  19. pRDA_186

    Plasmid
    #133458
    Purpose
    U6 promoter expresses customizable Spyo-guide; PGK promoter expresses blasticidin resistance and 2A site provides EGFP
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133458-plasmid-map-sequence-id-261410

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  20. Blat-BSD

    Plasmid
    #163791
    Purpose
    The plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.
    Depositor
    Yongming Wang
    Article
    Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    163791-plasmid-map-sequence-id-328000

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  21. pBSR (mCherry-surrogateTarget-bsr)

    Plasmid
    #66950
    Purpose
    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.
    Depositor
    Silvestro Conticello
    Article
    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.
    Insert
    blasticidin
    Use
    CRISPR, Mouse Targeting, and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    beta-actin
    Available since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    66950-plasmid-map-sequence-id-115273

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  22. Plenti-ARE-HSVTK-luc

    Plasmid
    #161789
    Purpose
    Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers
    Depositor
    Hans-Guido Wendel
    Article
    Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi
    Inserts
    Luciferase-T2A-HSV-TK
    mCherry P2A blasticidin
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and Murine Gclc ARE
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    161789-plasmid-map-sequence-id-324302

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  23. pDRM166 LKB (Luciferase mKate2 Blast)

    Plasmid
    #183502
    Purpose
    Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.
    Depositor
    Wayne Tilley
    Article
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    mKate2
    BSD
    Use
    Lentiviral and Luciferase
    Tags
    P2A linker and SV40 NLS with GGS linker
    Expression
    Mammalian
    Mutation
    Promoter
    MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    183502-plasmid-map-sequence-id-362519

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  28. pLenti-mCherry-Cre-blast

    Plasmid
    #179390
    Purpose
    Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.
    Depositor
    Moshe Szyf
    Article
    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:
    Insert
    mCherry (P2A) Cre recombinase
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179390-plasmid-map-sequence-id-451660

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  30. epi-BE4max

    Plasmid
    #135975
    Purpose
    Expresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
    Depositor
    Yongming Wang
    Article
    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135975-plasmid-map-sequence-id-292615

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  33. epi-ABEmax

    Plasmid
    #135974
    Purpose
    Expresses ABEmax, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
    Depositor
    Yongming Wang
    Article
    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only

  37. SpRY

    Plasmid
    #198544
    Purpose
    Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpRY
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198544-plasmid-map-sequence-id-390296

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  39. EGFP in pDBEcR-pIND(SP1)/Bla

    Plasmid
    #201557
    Purpose
    Ecdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance.
    Depositor
    Randy Poon
    Article
    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754
    Insert
    DBEcR and EGFP
    Use
    Unspecified; Sleeping beauty transposon
    Tags
    DBEcR-myc and FLAG-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and pIMD(SP1)
    Available since
    July 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    201557-plasmid-map-sequence-id-400936

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  46. pRDA_103

    Plasmid
    #133460
    Purpose
    H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133460-plasmid-map-sequence-id-261409

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  48. Sc++

    Plasmid
    #198545
    Purpose
    Expresses human codon-optimized Sc++ and blasticidin resistance: EFS promoter-Sc++-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    Sc++
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198545-plasmid-map-sequence-id-388902

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  49. SpG

    Plasmid
    #198543
    Purpose
    Expresses human codon-optimized SpG and blasticidin resistance: EFS promoter-SpG-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpG
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198543-plasmid-map-sequence-id-388887

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  50. pDest-pol2-Smarcd1-Myc

    Plasmid
    #227716
    Purpose
    Expresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter. Has a Blasticidin resistance gene for stable cell line generation and Ampicilin for plasmid propagation.
    Depositor
    Kent Hunter
    Article
    Ross et al bioRxiv 2024.01.24.577061
    Insert
    Smarcd1 (Smarcd1 Mouse)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    pol2
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    227716-plasmid-map-sequence-id-450967

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Showing: 101 - 150 of 464 results