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pHR-tdMCP-YFP

Plasmid

#99151

Purpose

Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vector

Depositor

Ron Vale

Article

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

Dimeric MS2-coat binding protein

Use

Lentiviral

Tags

eYFP

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP720

Plasmid

#45460

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Expression

Bacterial

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGCDNsam-Foxa3

Plasmid

#33005

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

forkhead box A3 (Foxa3 Mouse)

Use

Retroviral

Available Since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only
pFLlR5

Plasmid

#149481

Purpose

Broad-Host range expression vector, RSF1010 origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LacI-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLlacO-1

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAW016-2

Plasmid

#85613

Purpose

Cas9-tracrRNA integration vector for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

cas9-tracrRNA

Expression

Bacterial

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pFRm43GW

Plasmid

#133748

Purpose

Three-fragment Multisite Gateway vector construction for red fluorescent seed selection in Arabidopsis thaliana

Depositor

Niko Geldner

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Type

Empty backbone

Expression

Plant

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-PLtetO1-tetR-T500

Plasmid

#45391

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

tet repressor

Expression

Bacterial

Promoter

PL-tetO1

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pDDGFP_LEU2D

Plasmid

#58352

Purpose

Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

Depositor

Simon Newstead

Article

Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

Type

Empty backbone

Tags

GFP-His

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pNL-EGFP/CMV/WPREdU3

Plasmid

#17579

Depositor

Jakob Reiser

Article

Ricks et al Stem Cells Dev. 2008 Jun . 17(3):441-50.

Insert

EGFP

Use

Lentiviral

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pSelect-6

Plasmid

#190243

Purpose

Encodes lacI- and GFP-targeting gRNAs for positive and negative selections of dCas9 activity.

Depositor

Marc Ostermeier

Article

Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

Insert

LacI

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pNL-EGFP/EF1alpha/WPREdU3

Plasmid

#18698

Depositor

Jakob Reiser

Article

Ricks et al Stem Cells Dev. 2008 Jun . 17(3):441-50.

Insert

EGFP

Use

Lentiviral

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG384 bFos-CFAST11

Plasmid

#130815

Purpose

Expresses bFos-CFAST11 in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

bFos-CFAST11

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBB550

Plasmid

#27396

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

dnaK, dnaJ, GroESL

Expression

Bacterial

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pIB2

Plasmid

#25451

Depositor

Benjamin Glick

Article

Sears et al Yeast. 1998 Jun 15;14(8):783-90.

Insert

Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)

Use

Pichia pastoris expression

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pCL-CTIG

Plasmid

#14901

Purpose

3rd generation lentiviral vector expressing tTA-VP16 and EGFP.

Depositor

Inder Verma

Article

Kafri et al Mol Ther. 2000 Jun . 1(6):516-21.

Insert

CMV-tTA-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

May 11, 2007

Availability

Academic Institutions and Nonprofits only

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pTEC20

Plasmid

#30179

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

mKate2

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)

Plasmid

#203908

Purpose

Encodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

Gal4UAS_dGFP_PGK_iRFP

Expression

Mammalian

Promoter

5xUAS and PGK

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW019-2

Plasmid

#85614

Purpose

Inducible dCas9 integration vector for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

dcas9

Expression

Bacterial

Promoter

PxylA (B. megaterium)

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2E2

Plasmid

#15788

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2E2 (UBE2E2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pCAG-rtTA

Plasmid

#163601

Purpose

Expresses rtTA in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

rtTA

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-wtFAKSPARK2

Plasmid

#195263

Purpose

express wtFAKSPARK2

Depositor

Xiaokun Shu

Article

Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

Insert

FAKSPARK2 sensor

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWBs02

Plasmid

#222478

Purpose

Gateway vector for 35s promoter-driven expression in Marchantia polymorpha carrying a sulfadiazine selection marker.

Depositor

Philip Carella

Article

Robinson et al J Exp Bot. 2024 Jun 2:erae256. doi: 10.1

Type

Empty backbone

Expression

Plant

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTEC27

Plasmid

#30182

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

tdTomato

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pWLlR5

Plasmid

#149492

Purpose

Broad-Host range expression vector, incW (pSa) origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LacI-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLlacO-1

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR GFP-CaaX

Plasmid

#113020

Purpose

for lentivirus production of GFP-CaaX

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

GFP-CaaX

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RGS-SENP5

Plasmid

#18053

Depositor

Edward Yeh

Article

Gong et al J Biol Chem. 2006 Jun 9. 281(23):15869-7

Insert

SENP5 (SENP5 Human)

Tags

RGS

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pTEC18

Plasmid

#30177

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

EBFP2

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEA183

Plasmid

#187873

Purpose

Expresses eGFP under the virB promoter.

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

eGFP

Expression

Bacterial

Promoter

Agrobacterium virB promoter

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWLxR5

Plasmid

#149490

Purpose

Broad-Host range expression vector, incW (pSa) origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWBs00

Plasmid

#222476

Purpose

Marchantia polymorpha transformation vector carrying sulfadiazine selection marker.

Depositor

Philip Carella

Article

Robinson et al J Exp Bot. 2024 Jun 2:erae256. doi: 10.1

Type

Empty backbone

Expression

Plant

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ2048.2

Plasmid

#140687

Purpose

3-color stripe (different sgRNAs)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

3-color stripe (different sgRNAs)

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RGS-SENP5m

Plasmid

#18715

Depositor

Edward Yeh

Article

Gong et al J Biol Chem. 2006 Jun 9. 281(23):15869-7

Insert

SENP5m (SENP5 Human)

Tags

RGS

Expression

Mammalian

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
pKAR5

Plasmid

#149461

Purpose

Broad-Host range expression vector, RK2 and pUC origin, AraC activator/repressor, PBAD promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

AraC-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGPI-SceI

Plasmid

#32060

Purpose

Construction of unmarked gene deletions. Suicide cloning vector designed to introduce a targeted I-SceI restriction site in the genome of Burkholderia

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Type

Empty backbone

Use

Mutagenesis plasmid

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPC myc hPOT1

Plasmid

#12387

Depositor

Titia de Lange

Article

Loayza et al Nature. 2003 Jun 26. 423(6943):1013-8.

Insert

Protection of Telomeres 1 (POT1 Human)

Use

Retroviral

Tags

Myc

Available Since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pHSS6hsILMi20

Plasmid

#102536

Purpose

Helper plasmid expressing Minos transposase mRNA (Drosophila hsp70 promoter)

Depositor

Michalis Averof, Charalambos Savakis

Article

Klinakis et al Insect Mol Biol. 2000 Jun;9(3):269-75.

Insert

Minos transposase

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF_LuxR

Plasmid

#140039

Purpose

Constutitive expression of LuxR protein.

Depositor

Yolanda Schaerli

Article

Barbier et al Mol Syst Biol. 2020 Jun;16(6):e9361. doi

Insert

pLuxL and LuxR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRMCD70

Plasmid

#167557

Purpose

Plasmid pWSK29 encoding E. coli LacZ' for making reporter fusion proteins for topology mapping

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1998 Jun;28(6):1211-22. d

Type

Empty backbone

Tags

LacZ'

Expression

Bacterial

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pIB4

Plasmid

#25453

Depositor

Benjamin Glick

Article

Sears et al Yeast. 1998 Jun 15;14(8):783-90.

Insert

Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)

Use

Pichia pastoris expression

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB528

Plasmid

#27390

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

lacIq

Expression

Bacterial

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+FucciOrange

Plasmid

#128427

Purpose

Contains insert allowing in-vivo visualization of Cdk1 protein (G1)

Depositor

Rusty Lansford

Article

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

FucciOrange

Expression

Mammalian

Promoter

CMV & SP6

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPAP004

Plasmid

#160911

Purpose

E.coli/ P.pastoris shuttle plasmid for episomal expression in P.pastoris

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

lacZ selection cassette

Expression

Yeast

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-SNAP-KanMX6

Plasmid

#87024

Purpose

Tag S. pombe genes in C terminal with SNAP

Depositor

Julien Berro

Article

Lacy et al Mol Biol Cell. 2017 Jun 28. pii: mbc.E17

Insert

SNAP

Expression

Bacterial and Yeast

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSICO-Flpo

Plasmid

#24969

Depositor

Tyler Jacks

Article

Young et al Proc Natl Acad Sci U S A. 2010 Jun 1. 10

Insert

Flp recombinase

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD/HisB-iRFP670

Plasmid

#45453

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Expression

Bacterial

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pACU2

Plasmid

#31223

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Type

Empty backbone

Expression

Insect

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
fIM1265

Plasmid

#132577

Purpose

pZA2-pLlacO-soxR

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Insert

pLlacO-soxR-TermT1 (soxR )

Expression

Bacterial

Promoter

pLlacO

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFKM1

Plasmid

#64950

Purpose

source of KmR FRT cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-KmR-FRT

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFNR5

Plasmid

#149483

Purpose

Broad-Host range expression vector, RSF1010 origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

NahR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PSalTTC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UbE2Q2

Plasmid

#15801

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2Q2 (UBE2Q2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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