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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,297 results for: CAD;

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Showing: 6361 - 6380 of 7297 results

  1. pCJ189

    Plasmid
    #162666
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162666-plasmid-map-sequence-id-321465

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  3. pCJ190

    Plasmid
    #162667
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162667-plasmid-map-sequence-id-321443

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  5. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid
    #112203
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W159H, S238F
    Promoter
    N/A
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112203-plasmid-map-sequence-id-473446

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  6. pT3-EF1aH-PIK3CA H1047R

    Plasmid
    #180031
    Purpose
    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.
    Depositor
    Xin Chen
    Article
    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv
    Insert
    PIK3CA H1047R (PIK3CA Human)
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    H1047R
    Promoter
    EF1a
    Available Since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180031-plasmid-map-sequence-id-357864

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  10. pGL4.22-PGK1-HRE::dLUC

    Plasmid
    #128095
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Article
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Insert
    Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Promoter
    inserted PGK1 hypoxia response elements (HRE) (x3)
    Available Since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128095-plasmid-map-sequence-id-249667

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  12. pSicoR p53

    Plasmid
    #12090
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF p53 shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Insert
    p53 shRNA (Trp53 Mouse)
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Mutation
    EGFP is expressed from this plasmid as a marker, …
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12090-plasmid-map-sequence-id-7226

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  18. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid
    #162877
    Purpose
    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM
    Depositor
    Frederic Meunier
    Article
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Insert
    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Insert consists of AA1-170
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162877-plasmid-map-sequence-id-321985

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  20. pAAV-Syn1-FLEX-mCh-T2A-FLAG-hMOR-WPRE

    Plasmid
    #166970
    Purpose
    Cre-dependent viral expression of the human mu opioid receptor (hMOR/Oprm1) and mCherry. hMOR contains an N-terminal FLAG tag.
    Depositor
    Matthew Banghart
    Article
    Liu et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    Homo sapiens opioid receptor mu 1 (OPRM1) (OPRM1 Human)
    Use
    AAV and Cre/Lox
    Tags
    FLAG, T2A, and mCherry
    Expression
    Mammalian
    Promoter
    hsyn
    Available Since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166970-plasmid-map-sequence-id-346867

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Showing: 6361 - 6380 of 7297 results