We narrowed to 23,082 results for: cas9
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Plasmid#47946PurposeExpresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, egl-13 NLS, and mEGFPExpressionWormPromoterhsp-16.48Available SinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI5M
Plasmid#113078PurposeExpresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseCRISPRExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
M-SPcas
Plasmid#48668PurposeMammalian S. pyogenes Cas9 expression, human optimizedDepositorInsertCas9
UseCRISPRTagsNLSPromoterCMVAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
p3s-Cas9HC
Plasmid#43945DepositorInsertCas9
UseCRISPRTagsHA epitope and NLSExpressionMammalianPromoterCMVAvailable SinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUB-Cas9
Plasmid#86556PurposeFor ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)DepositorInsertCas9
UseCRISPRExpressionPlantMutationCas9 is codon optimized for C. reinhardtiiPromoterUBIQUITIN10Available SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cas9-162A
Plasmid#84918Purposefor in vitro transcription of Cas9 with polyA tail encodedDepositorInsertCas9
TagsFlag, NLS, and polyA tailExpressionMammalianPromoterCAGAvailable SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB-MeCP2
Plasmid#110821PurposeImproved dCas9 repressor-dCas9-KRAB-MeCP2DepositorInsertdCas9-KRAB-MeCP2
ExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgRNA
Plasmid#124844PurposeVector for Cre-dependent expression of SaCas9DepositorInsertSaCas9, gRNA scaffold
UseAAV, CRISPR, and Mouse TargetingTagsNLS and NLS-3xHAAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSU-araC-Cas9
Plasmid#120423PurposeMultigene editing in the Escherichia coli genome using the CRISPR-Cas9 systemDepositorInsertCas9
UseCRISPRExpressionBacterialPromoteraraBADAvailable SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x3
Plasmid#58767PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCasKP-hph
Plasmid#117232PurposeBacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella PneumoniaeDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)
Plasmid#90086PurposeThis plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNADepositorInsertCas9
UseCRISPR and LentiviralTagsDestabilized Domain and FlagExpressionMammalianPromoterEFSAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-dCas9-5xPlat2AflD
Plasmid#82560PurposeExpression vector for Cas9 peptide array (linker length: 22aa).DepositorInsertdCas9-5xPlat2AflD
ExpressionMammalianAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX165-LZ3 Cas9
Plasmid#140561PurposeExpresses LZ3 Cas9 in mammalian cellsDepositorInsertCas9
MutationN690C T769I G915M N980KAvailable SinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#106249PurposeLentiviral vector with puro selection for expression of S. aureus dCas9-KRABDepositorInsertdead S. aureus Cas9 KRAB T2A PuroR
UseCRISPR and LentiviralTagsHA-NLS and NLS-KRABExpressionMammalianMutationD10A and N580APromoterhUbCAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIK86
Plasmid#47933PurposeC. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.DepositorInsertCas9
UseCRISPR and UnspecifiedPromoterT7Available SinceOct. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9P35S-H
Plasmid#66189Purposeexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selectionDepositorInsertCas9
ExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotercauliflower mosaic virus 35S promoter (P35S)Available SinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Multiplex CRISPR/Cas9 Assembly System Kit
Plasmid Kit#1000000055PurposePlasmids to create all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs for genome engineering using Cas9 nuclease or Cas9 D10A nickase.DepositorApplicationGenome EditingVector TypeMammalian ExpressionEditing TypeCRISPRAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PXL
Plasmid#75349PurposePX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.DepositorInsertCas9
UseCRISPRExpressionMammalianPromoterU6Available SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
M-SPcas
Plasmid#48668PurposeMammalian S. pyogenes Cas9 expression, human optimizedDepositorInsertCas9
UseCRISPRTagsNLSPromoterCMVAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only