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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 1701 - 1750 of 11733 results

  4. HSC1-GiP

    Plasmid
    #58254
    Purpose
    This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Human EF1-alpha
    Available Since
    Oct. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    58254-plasmid-map-sequence-id-321491

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  12. TBC1D7

    Plasmid
    #32047
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TBC1D7 (TBC1D7 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Available Since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. GLuc-PM-Venus

    Plasmid
    #164785
    Purpose
    Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein
    Depositor
    Laszlo Hunyady
    Article
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Insert
    Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Gaussia princeps, Human)
    Tags
    GLuc and mVenus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164785-plasmid-map-sequence-id-326677

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  14. pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]

    Plasmid
    #74102
    Purpose
    plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)
    Depositor
    Ben Szaro
    Article
    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:
    Inserts
    Xenopus laevis beta-tubulin gene promoter region and 5'UTR
    Green Lantern Green Fluorescent Protein
    Rabbit beta 1-globin gene 3'UTR
    Expression
    Bacterial
    Available Since
    May 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74102-plasmid-map-sequence-id-148113

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  19. pAM4787

    Plasmid
    #120088
    Purpose
    Cyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).
    Depositor
    Susan Golden
    Article
    Chen et al Microbiology. 2016 Dec;162(12):2029-2041
    Insert
    Plasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
    Use
    Other
    Available Since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120088-plasmid-map-sequence-id-234398

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  21. pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73040
    Purpose
    Express Nluc and copGFP dual reporter in mammalian cells
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    PGK (phosphoglycerate kinase 1)
    Available Since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73040-plasmid-map-sequence-id-136039

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  25. G protein alpha-q-CFP

    Plasmid
    #66081
    Purpose
    This G protein alpha-q construct contains internal insertions of CFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Insert
    G protein alpha-q-EE-CFP (Gnaq Mouse)
    Tags
    CFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available Since
    July 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66081-plasmid-map-sequence-id-118921

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  48. pAAV-UbC-eGFP-F

    Plasmid
    #71545
    Purpose
    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.
    Depositor
    Pantelis Tsoulfas
    Insert
    eGFP-F
    Use
    AAV
    Tags
    contains 20- amino-acid farnesylation signal from…
    Expression
    Mammalian
    Promoter
    UbC
    Available Since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    71545-plasmid-map-sequence-id-132622

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Showing: 1701 - 1750 of 11733 results