181,545 results
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Plasmid#214895PurposeExpresses equimolar Lipid Droplet- and Lysosome- targeted split-FAST fragments for Short-range LD-LY Contact sitesDepositorInsertSPLICSFAST-LDh-LY-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_CAG-mStayGold
Plasmid#229802PurposeBxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGoldDepositorInsertmStayGold
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-320_Cacnes_ori
Plasmid#225602PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli oriDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pC2HP
Plasmid#63735Purposeprey plasmid for an adapted two-hybrid system for C. albicansDepositorTypeEmpty backboneUseC. albicans prey plasmidTagsFLAG, NLS, and VP domainExpressionMutationPromoterMet3Available sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)
Plasmid#204619PurposeExpression vector of Cascade and Cas3. Puromycin selectable.DepositorInsertCas7, Cas5, Cas8, Cas11, Cas6, and Cas3
UseCRISPRTagsEach protein has C- and N-terminal SV40 NLSExpressionMutationPromoterEF1aAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TGFB1
Plasmid#116799PurposeLentiviral expression of TGFB1DepositorInsertTGFB1 (TGFB1 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A RIGI WT
Plasmid#167289PurposeLentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-IDepositorInsertDDX58 (RIGI Human)
UseLentiviralTagstagBFPExpressionMammalianMutationPromoterSFFVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2:35S-TMV-Rep-MP
Plasmid#106217PurposeProvides CaMV 35S and 5' part of Tobacco mosaic virus (RdRp polymerase with intron, movement protein and sgCP promoter) as level 0 GoldenBraid part (GGAG-AATG)DepositorInsertInfectious clone of Tobacco mosaic virus, 5' part
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PKIA_WT
Plasmid#82153PurposeGateway Donor vector containing PKIA , part of the Target Accelerator Plasmid Collection.DepositorInsertPKIA (PKIA Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRE3G-PE2-P2A-BFP
Plasmid#231580PurposeExpresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA proteinDepositorInsertPE2-P2A-BFP
UseLentiviralTagsP2A-BFPExpressionMammalianMutationPromoterTRE3GAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO GFP-Zeo DEST (719-1)
Plasmid#17431Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-ZeoDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO GFP-Zeo DEST (719-1)
Plasmid#17431Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-ZeoDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
Plasmid#207863PurposeAAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNADepositorInsertNpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
IL13 promoter luciferase
Plasmid#11784DepositorInsertIL-13 promoter (IL13 Human)
UseLuciferaseTagsluciferaseExpressionBacterialMutationPromoterAvailable sinceMay 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
IL13 promoter luciferase
Plasmid#11784DepositorInsertIL-13 promoter (IL13 Human)
UseLuciferaseTagsluciferaseExpressionBacterialMutationPromoterAvailable sinceMay 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Annexin-12
Plasmid#55276DepositorInsertAnnexin (A4) (ANXA4 Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-Sec16A
Plasmid#36155PurposeExpresses Sec16A as a GFP fusionDepositorInsertSec16A (SEC16A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PpipB-GFPLVA
Plasmid#23344DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PpipB-GFPLVA
Plasmid#23344DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCI152-pLenti-SiT-Cas12a
Plasmid#128405PurposeExpress both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-S33Y Beta-catenin
Plasmid#19286PurposeMammalian expression of human beta-catenin with Flag tag and S33Y mutationDepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsFlagExpressionMammalianMutationS33Y (presumptive GSK3β phosphorylation site)PromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-S33Y Beta-catenin
Plasmid#19286PurposeMammalian expression of human beta-catenin with Flag tag and S33Y mutationDepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsFlagExpressionMammalianMutationS33Y (presumptive GSK3β phosphorylation site)PromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pM-CBE
Plasmid#196293Purpose35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GPDepositorInsertFull length TSWV M antigenome encoding CBE in place of viral GP
UseCRISPRTags3×flagExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-AckRS
Plasmid#137976Purposesequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansionDepositorInsertAckRS and pylTcua
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB2E
Plasmid#195288PurposeBicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selectionDepositorInsertHSV-TK EGFP 2A Puro
UseTagsExpressionMammalianMutationPromoterEFSAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-JAG1-Fc(DAPA)-AviTag-6xHis
Plasmid#157024PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertJAG1 (JAG1 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
SunTagng22aa
Plasmid#106438PurposeEncodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.DepositorInsertNOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-RiboL1-jGCaMP8m
Plasmid#169248PurposeAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8m protein.DepositorInsertRiboL1-jGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BRCA1
Plasmid#119007PurposeMammalian expression of human BRCA1 and BFPDepositorInsertBRCA1 (BRCA1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
TGT-His
Plasmid#138201PurposeExpresses E. Coli TGT with a C-terminal His TagDepositorInsertE Coli tRNA Guanine Transglycosylase-His
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only