178,426 results
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Plasmid#42876PurposeBacterial expression of Cas9 nuclease, tracrRNA and crRNA guideDepositorInserttracr/Cas9
UseCRISPR; E.coliExpressionBacterialAvailable sinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9n(BB)-2A-Puro (PX462)
Plasmid#48141PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62987DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
8xGTIIC-luciferase
Plasmid#34615PurposeYAP/TAZ-responsive synthetic promoter driving luciferase expressionDepositorInsertsynthetic TEAD luciferase reporter
UseLuciferaseAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1 MCS-BirA(R118G)-HA
Plasmid#36047PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tagDepositorPublicationHas serviceCloning Grade DNAInsertBirA(R118G)-HA
TagsHAExpressionMammalianMutationR118G (highly promiscuous form)Available sinceApril 13, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tet-pLKO-neo
Plasmid#21916Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas serviceCloning Grade DNATypeEmpty backboneUseLentiviralAvailable sinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-Cre:GFP
Plasmid#13776PurposeMammalian expression of Cre recombinase fused to GFPDepositorInsertCre-GFP fusion protein
UseCre/LoxTagsGFPExpressionMammalianPromoterCAGAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX304
Plasmid#25890Purpose3rd generation lentiviral Gateway destination vector. Blasticidin selection, V5 tag.DepositorHas serviceCloning Grade DNATypeEmpty backboneUseGateway Cloning and LentiviralTagsV5ExpressionMammalianAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MCS-BioID2-HA
Plasmid#74224PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tagDepositorHas serviceCloning Grade DNATypeEmpty backboneTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-DIO-mCherry (AAV8)
Viral prep#50459-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (AAV Retrograde)
Viral prep#105540-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. Expression of EGFP-Cre from hSyn promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-tdTomato (codon diversified) (AAV Retrograde)
Viral prep#59462-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. tdTomato expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (codon diversified)Available sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)
Plasmid#71814PurposeExpresses high specificity SpCas9. Px330-like plasmid.DepositorHas serviceCloning Grade DNAInsertenhanced specificity Cas9 (1.1)
UseCRISPRTags3xFLAGExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-hTERT-IRES-hygro
Plasmid#85140PurposeLentiviral expression of hTERT, used to create immortalized cell linesDepositorAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-puro
Plasmid#39481PurposeTetracycline inducible 3rd generation lentiviral vectorDepositorHas serviceCloning Grade DNATypeEmpty backboneUseLentiviralTagsV5 and 6XHisExpressionMammalianPromoterCMVAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti CMV Puro DEST (w118-1)
Plasmid#17452Purpose3rd gen lentiviral Gateway destination vector, expression, CMV promoter, PuroDepositorPublicationHas serviceCloning Grade DNATypeEmpty backboneUseGateway Cloning and LentiviralExpressionMammalianAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCBASceI
Plasmid#26477PurposeI-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI siteDepositorInsertpCBASceI
TagsHAExpressionMammalianAvailable sinceOct. 20, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-HA-Ubiquitin-K63
Plasmid#17606PurposeMammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to argininesDepositorPublicationInsertUbiquitin C (UBC Human)
TagsHAExpressionMammalianMutationK63 only, other lysines mutated to arginines. Enh…Available sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-CreERT2
Plasmid#14797PurposeMammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)DepositorInsertCre-ERT2 fusion protein
UseCre/LoxExpressionMammalianPromoterCAGAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1 mycBioID
Plasmid#35700PurposeTo fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tagDepositorPublicationHas serviceCloning Grade DNAInsertBirA
TagsMycExpressionMammalianMutationR118G (highly promiscuous form)PromotercmvAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Human GeCKO Lentiviral sgRNA Library v2 (lentiGuide-Puro)
Pooled library#1000000049PurposeCRISPR gRNA knockout pooled library for targeting the human genomeDepositorAvailable sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by