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pAAV FLEX Synaptophysin GFP
Plasmid#137188PurposeCre-dependent expression of synaptophysin-GFPDepositorInsertSynaptophysin GFP (Syp Mouse)
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-HA-Ebp1
Plasmid#67792PurposeMammalian expression of Ebp1 tagged with HADepositorInsertEbp1 (PA2G4 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip4
Plasmid#58925PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 CrkII
Plasmid#50731Purposemammalian expression of CrkIIDepositorInsertCrkII (CRK Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-CrkII
Plasmid#50024PurposepENTR vector for mouse Crk-IIDepositorInsertCrk-II (Crk Mouse)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3B09
Plasmid#70998PurposeGateway entry cloneDepositorInsertHrb87F (Hrb87F Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Synapto-pHluorin
Plasmid#168510PurposeExpression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.DepositorInsertSynaptophysin-pHluorin (Syp Mouse)
UseTagspHluorinExpressionMammalianMutationPromoterCAGAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-mRuby3
Plasmid#179727PurposeLentiviral expression of SYP-mRuby3 for use as a synaptic vesicle markerDepositorInsertSYP (Syp Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcFLAG-Grap2-WT
Plasmid#74511PurposeOverexpression of Grap2 in mammalian cellsDepositorInsertGrap2 (GRAP2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA CrkII Y221F
Plasmid#50732Purposemammalian expression of CrkII Y221F mutantDepositorInsertCrkII Y221F (CRK Human)
UseTagsExpressionMammalianMutationY221FPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAB043c
Plasmid#79223PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ lambda cI-p38-alpha; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-1 Jip4 (1-272)
Plasmid#59002PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsGstExpressionBacterialMutationJip4 (aa1-272)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip4 (1-272)
Plasmid#58928PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationJip4 (1-272aa)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip4 (1-151)
Plasmid#58926PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationJip4 (1-151aa)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip4 (1-212)
Plasmid#58927PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationJip4 (1-212aa)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-tdTomato-2A-mSyp::EGFP
Plasmid#191213PurposeExpresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.DepositorInserttdTomato-T2A-mSyp::EGFP (Syp Mouse)
UsePcagenTagsmSyp::EGFPExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcFLAG-Grap2-R83E
Plasmid#74512PurposeOverexpression of Grap2 R83E in mammalian cellsDepositorInsertGrap2 (GRAP2 Human)
UseTagsFLAGExpressionMammalianMutationR83EPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ERKKTRClover
Plasmid#59138PurposeORF encoding ERK KTR mClover flanked by Gateway sequencesDepositorInsertERK Kinase Translocation Reporter (MAPK1 Human, Mouse)
UseTagsExpressionMutationPromoterAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-synaptophysin-Lime
Plasmid#201016PurposeExpresses Lime in synaptic vesicle lumen by fusion to synaptophysinDepositorInsertSynaptophysin-Lime (Syp Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre
Plasmid#134308PurposeExpresses Synaptophysin-TdTomato and also tamoxifen dependant Cre (OHT-Cre, aka Cre-ER)DepositorInsertsUseTagsTdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFlag-tdMCP-Erk2 (pErk reporter)
Plasmid#226167PurposeUsed to transiently express the pErk reporter in LABEL-seq MAPK activity assays.DepositorInsert3xFlag-tdMCP-Erk2 (MAPK1 Human)
UseTags3xFlag-tdMCPExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-hSyn-mSyp-RT1-24xGCN4
Plasmid#106300PurposeThe plasmid encodes mouse Synaptophysin sparsely labeled with SunTag24x_v4.DepositorInsertmSyp-RT1-24xGCN4 (Syp Mouse)
UseTags24xGCN4ExpressionMammalianMutationPromoterhuman Synapsin1Available sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CRK_SH3
Plasmid#178472PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCRK_SH3 (CRK Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-HA-ERK2 GOF
Plasmid#53200PurposeExpresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vectorDepositorInsertERK2 (MAPK1 Human)
UseTagsGST and HAExpressionBacterialMutationR67S/D321NPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSAD-5PSD95-EGFP-SynPhRFP
Plasmid#217979PurposeG-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFPDepositorUseG-deleted rabiesTagsEGFP and RFPExpressionMutationPromoterNoneAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_MAPK14-P2A-Hygro_Barcode
Plasmid#170239PurposeBarcoded lentiviral vector to express MAPK14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertMAPK14 (MAPK14 Human)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-HA-ERK2 R67S
Plasmid#53201PurposeExpresses GST and HA tagged human ERK2 R67S from bacterial expression vectorDepositorInsertERK2 (MAPK1 Human)
UseTagsGST and HAExpressionBacterialMutationR67SPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFIN-RK-GFP-WPRE
Plasmid#44358DepositorInsertRK-GFP (MAPK14 Human, Synthetic)
UseLentiviralTagsExpressionMutationPromoterhuman rhodopsin kinase (pos 2557-2853)Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
MEF2A-Sensor
Plasmid#208928Purposein vitro MAPK-Sensor for luminescent detection of ERK and p38-activityDepositorInsertMef2a docking sequence+Phospho site (MEF2A Human)
UseTagsHISx6, MBP, and SmbitExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-HA-ERK2 D321N
Plasmid#53202PurposeExpresses GST and HA tagged human ERK2 D321N from bacterial expression vectorDepositorInsertERK2 (MAPK1 Human)
UseTagsGST and HAExpressionBacterialMutationD321NPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX Crk-SH2
Plasmid#46418DepositorInsertCrk (CRK Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 1-124PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
JPF0419d
Plasmid#124020PurposeEncodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vectorDepositorInsertPB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CRK-1/2
Plasmid#91367PurposeProtein expression and purification of human SH3 domain construct CRK-1/2DepositorInsertCRK-1/2 (CRK Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
JPF0419v
Plasmid#124021PurposeEncodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vectorDepositorInsertPB_pCAG-Lyn-iRFP713_pCAG-NES-mAzamiGreen_pCAG-p38KTR-mCerulean_pCAG-H2B::mScarlet
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRAP2_SH3
Plasmid#110016PurposeProtein expression and purification of GRAP2_SH3DepositorInsertGRAP2_SH3 (GRAP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHrb98_AA
Plasmid#148211PurposeInsect Expression of DmHrb98DepositorInsertDmHrb98 (Hrb98DE Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc.1B-EGFP-DmHrb98
Plasmid#66595PurposeExpresses EGFP-tagged Hrb98 in Drosophila cellsDepositorInsertHrb98 (Hrb98DE Fly)
UseDrosophila expressionTagsEGFPExpressionMutationPromoterActin 5CAvailable sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3G08
Plasmid#71044PurposeGateway entry cloneDepositorInsertHrb98DE (Hrb98DE Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
MK11_HUMAN_D0
Plasmid#79692PurposeThis plasmid encodes the kinase domain of MK11. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMK11 (MAPK11 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK11 gRNA (BRDN0001147606)
Plasmid#77482Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK11DepositorInsertMAPK11 (MAPK11 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK11 gRNA (BRDN0001146070)
Plasmid#77483Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK11DepositorInsertMAPK11 (MAPK11 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK11 gRNA (BRDN0001147839)
Plasmid#77484Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK11DepositorInsertMAPK11 (MAPK11 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCJ190
Plasmid#162667PurposepET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ189
Plasmid#162666PurposepET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ191
Plasmid#162668PurposepET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF3321
Plasmid#144797PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPA2G4 (PA2G4 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAPK14 gRNA (BRDN0001145809)
Plasmid#77923Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK14DepositorInsertMAPK14 (MAPK14 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK14 gRNA (BRDN0001148417)
Plasmid#77924Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK14DepositorInsertMAPK14 (MAPK14 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only