169,450 results
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Plasmid#164142PurposeRetroviral expression vector with hygromycin resistanceDepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only
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CROPseq-Guide-Puro-mCherry2
Plasmid#219679PurposeCROPseq vector based on #86708 with an additional mCherry2 fluorescent geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsEF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNAExpressionMammalianAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoTPNOX
Plasmid#87854PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertmitoTPNOX
TagsC-terminal FLAG tag with a linker and mitochondri…ExpressionBacterialAvailable SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-q ONE-GO
Plasmid#204336PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-q ONE-GO
UseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
1GFP/RNase H1 D210N
Plasmid#174448PurposeExpress GFP-tagged RNase H1 D210N in E. coli.DepositorInsertRNase H1 (RNASEH1 Human)
TagsHis-tag, GFPExpressionBacterialMutationD210NPromoterT7 promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hALDOA
Plasmid#210809PurposeFor transient transfectionDepositorAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEND-320_Cacnes_ori
Plasmid#225602PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli oriDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2B tdTomato
Plasmid#116870PurposeNuclear localized tdTomatoDepositorHas ServiceAAV RetrogradeInsertH2B tdTomato
UseAAVAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-MAPPER
Plasmid#117721PurposeMAPPER Selectively Labels ER-PM JunctionsDepositorInsertMAPPER
TagsGFPExpressionMammalianAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-AsCpf1-Blast
Plasmid#84750PurposeExpresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertAsCpf1
UseCRISPR and LentiviralTagsNLS-3xHA (C terminal on insert)ExpressionMammalianPromoterEFS-NSAvailable SinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro-Scrambled
Plasmid#47541PurposeTet inducible scrambled shRNA.DepositorInsertScrambled shRNA
UseLentiviral and RNAiExpressionMammalianPromoterH1Available SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEND-184_Cacnes_mCherry
Plasmid#225610PurposeReplicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.DepositorInsertmCherry
UseSynthetic BiologyTagsV5-6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-RpHLuorin2
Plasmid#171720PurposeLysosomal expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
Tagsratiometric pHluorin2ExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV d14GFP Donor
Plasmid#31475DepositorInsertSFFV d14GFP
UseLentiviralMutationDeleted 14 amino acids on the c terminus of eGFPAvailable SinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoA-91-NLuc
Plasmid#207151PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
ExpressionMammalianMutationNLuc inserted at position L(91)GAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoB-91-NLuc
Plasmid#207152PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
ExpressionMammalianMutationNLuc inserted at position L(91)GAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
mRFP-Lact-C2
Plasmid#74061PurposeFluorescent biosensor for phosphatidylserine.DepositorAvailable SinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PDPK1
Plasmid#23801DepositorInsertPDPK1 (PDPK1 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pACYC-GroEL/ES-TF
Plasmid#83923PurposeCo-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.DepositorInsertsGro EL/ES
Trigger Factor
ExpressionBacterialPromoterT7Available SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)
Plasmid#185911PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…PromoterCMV and T7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVS1-VIR1
Plasmid#138192PurposeVirulence helperDepositorInsertvir gene cluster
UseVir gene expressionAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEND-350_Cacnes_ori
Plasmid#225605PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-His6-1_HBD
Plasmid#171869PurposeExpresses a specific DNA-RNA hybrid sensor protein with the HBD domain of RNase H1 in bacterial cellsDepositorInsertHybrid binding domain of Rnase H1
TagsGST- and His10-tagExpressionBacterialMutationWTAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE142
Plasmid#118494Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsGFP2
ExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3
Plasmid#171588PurposeLentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoterDepositorInsertsBxb1 integrase
iCasp9
BFP
rtTA3G
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PKM2
Plasmid#23757DepositorInsertPKM2 (PKM Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA
Plasmid#190163PurposeMicroglia-targeted transgene expression.DepositorInsertsmIba1 promoter, 1.7-kb, microglia-specific promoter
miR-9-Tx4, 4 repeated miRNA target sites for miR-9
miR-129-2-3p-Tx4, 4 repeated miRNA target sites for miR-129-2-3p
UseAAVAvailable SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX317-EGFP
Plasmid#115439PurposeConstitutive lentiviral expressionDepositorInsertEGFP
UseLentiviralTagsV5ExpressionMammalianPromoterEF1aAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VEGFR1-V5/HIS
Plasmid#205616PurposeExpression of human VEGFR1 receptor tyrosine kinase in mammalian cellsDepositorAvailable SinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
HyPer7.2DAAO-NLS
Plasmid#168302PurposeChemogenetic fluorescent H2O2 reporter targeted to the nucleusDepositorInsertHyPer7.2DAAO-NLS
ExpressionMammalianPromoterCMVAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only