We narrowed to 5,632 results for: chia

1. pXTK037 [Con-Pro_glpT-Ecoli_PartType5]

   Plasmid

   #229274

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_glpT-Ecoli_PartType5

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   glpT Promoter (E coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-Impact-T4gp61(I342G)

   Plasmid

   #228208

   Purpose

   Expression in BL21(DE3) of T4 primase I342G

   Depositor

   Stephen Benkovic

   Article

   Valentine et al Biochemistry. 2001 Dec 18;40(50):15074-8

   Insert

   61 (61 Escherichia phage T4)
   

   Use

   Tags

   Sce VMA intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   Mutated isoleucine 342 to glycine (I342G), improv…

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ECMAB - pET22b Ag43 160N 6H Mms6

   Plasmid

   #225539

   Purpose

   Construction of extracellular magnetite accumulating bacterial systems.

   Depositor

   Urartu Seker

   Article

   Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

   Inserts

   Iron binding protein
   Autotransporter protein
   

   Use

   Tags

   no

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSFDUET-1_VNp-anti-Lysozyme fAb

   Plasmid

   #214951

   Purpose

   Bacterial expression of anti-chicken lysozyme (folded) Fab light and heavy chain VNp fusions

   Depositor

   Dan Mulvihill

   Article

   Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

   Inserts

   VNp-anti-Lysozyme Fab heavy chain fusion
   VNp-anti-Lysozyme Fab light chain fusion
   

   Use

   Tags

   VNp

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAGGS-Twin-strep-TEV-DGKδ2

   Plasmid

   #223720

   Purpose

   Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   DGKD (DGKD Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted start codon

   Promoter

   CAG and chicken β-actin promoter

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAGGS-PHOSPHO1-TEV-Twin-Strep

   Plasmid

   #222015

   Purpose

   Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   stop codon (TGA) is deleted

   Promoter

   chicken β-actin promoter

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SBC015898

   Plasmid

   #226285

   Purpose

   Expresses PhCFAT, ObEGS1, and ObEOMT1 from trc promoter. Biosynthesis of methylated phenylpropenes from monolignol precursors.

   Depositor

   Rainer Breitling

   Article

   Chua et al Metab Eng Commun. 2024 May 15;18:e00237.

   Inserts

   Coniferyl alcohol acyltransferase
   Eugenol synthase 1
   Eugenol O-methyltransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PtetA-PLacO3O1 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+

   Plasmid

   #225645

   Purpose

   tetA and LacO3O1 promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

   Depositor

   Andre Sanches Ribeiro

   Article

   Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

   Insert

   tetR/tetA and LacO3O1 in tandem conformation
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   tetA and LacO3O1 promoters

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PLacO3O1-PtetA 250 bp with pBELO_L6-PT3-T4_CAMR_TAN+

   Plasmid

   #225648

   Purpose

   LacO3O1 and tetA promoters in tandem conformation with 250bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

   Depositor

   Andre Sanches Ribeiro

   Article

   Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

   Insert

   LacO3O1 and tetR/tetA in tandem conformation
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   LacO3O1 and tetA promoters

   Available since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PLacO3O1-PtetA 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid

    #225647

    Purpose

    LacO3O1 and tetA promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

    Depositor

    Andre Sanches Ribeiro

    Article

    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

    Insert

    LacO3O1 and tetR/tetA in tandem conformation
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    LacO3O1 and tetA promoters

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PtetA-PLacO3O1 350 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid

    #225646

    Purpose

    tetA and LacO3O1 promoters in tandem conformation with 350bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

    Depositor

    Andre Sanches Ribeiro

    Article

    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

    Insert

    tetR/tetA and LacO3O1 in tandem conformation
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    tetA and LacO3O1 promoters

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pACYC-yhaM-RFP

    Plasmid

    #207651

    Purpose

    Overexpression of native L-cysteine desulfidase and RFP in E. coli MG1655

    Depositor

    Benjamin Woolston

    Article

    Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

    Insert

    yhaM
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMCSG7-10xHis-ecRNHI*

    Plasmid

    #219523

    Purpose

    bacterial expression of cysteine-free variant of the E. coli ribonuclease HI gene

    Depositor

    Arthur Palmer

    Article

    Bhattacharya et al J Biomol NMR. 2024 Jun 10. doi: 10.1007/

    Insert

    cysteine-free variant of the E. coli ribonuclease HI gene
    

    Use

    Tags

    N terminal 10xHis Tag

    Expression

    Bacterial

    Mutation

    Cysteine free variant

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBa.Halo-KIF1A tail

    Plasmid

    #218800

    Purpose

    Mammalian expression of rat Kif 1A

    Depositor

    Marvin Bentley

    Article

    Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

    Insert

    Kif 1A (Kif1a Rat)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    May 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMRMTK-clo1

    Plasmid

    #203919

    Purpose

    Cloning plasmid containing the BHR1 origin insert with a chloramphenicol resistance marker

    Depositor

    Nathan Crook

    Article

    van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

    Insert

    BHR1 Origin of Replication
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    N/A

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pQE-80L 2xHis-Rab12 Q101L

    Plasmid

    #207347

    Purpose

    Bacterial expression of Rab12

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    Rab12 (RAB12 Human)
    

    Use

    Tags

    2xHis

    Expression

    Bacterial

    Mutation

    Q101L

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmcb306-GFP-Rab12-mito

    Plasmid

    #207349

    Purpose

    Mammalian expression of Rab12-mito

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    Rab12-mito (RAB12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYG205

    Plasmid

    #200836

    Purpose

    Transcribes rapZ from the arabinose-inducible PBAD promoter; RapZ directs RNase E to cleave fusion RNAs transcribed from pYG215 or derivatives

    Depositor

    Boris Görke

    Article

    Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.

    Insert

    rapZ (rapZ Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P-BAD

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTP-PCR C1

    Plasmid

    #189618

    Purpose

    Cloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

    Insert

    Mitochondrial Genome of Thalassiosira pseudonana
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Minor additional mutations described in manuscript

    Promoter

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-C-TEV-HAT

    Plasmid

    #202597

    Purpose

    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    TEV cleavable site (ENLYFQG) and poly-histidine a…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAGGS-N-TEV-HAT

    Plasmid

    #202598

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    TEV cleavable site (ENLYFQG) and poly-histidine a…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBAD-P19-EGFP

    Plasmid

    #196609

    Purpose

    siRNA vector expressing TMV P19 and a 720-long EGFP sequence as inverted repeat

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Insert

    p19 + EGFP inverted repeat sequence
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    AraC

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBAD-P19-NP

    Plasmid

    #196608

    Purpose

    siRNA vector expressing TMV P19 and a 425bp-long NP sequence as inverted repeat

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Insert

    p19 + Influenza virus NP inverted repeat
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTP-PCR C4

    Plasmid

    #189621

    Purpose

    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

    Insert

    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Repeat region removed, and minor additional mutat…

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTP-PCR C2

    Plasmid

    #189619

    Purpose

    Cloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

    Insert

    Mitochondrial Genome of Thalassiosira pseudonana
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Minor additional mutations described in manuscript

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMC-6-AmCyan-P2A-NptII-9

    Plasmid

    #197742

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    AmCyan-P2A-NptII bicistronic CDS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. NeuA.pJL1

    Plasmid

    #199115

    Purpose

    In vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)

    Depositor

    Michael Jewett

    Article

    Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102

    Insert

    N-acylneuraminate cytodiylyltransfease (NeuA)
    

    Use

    Tags

    Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Lenti-guide-puro mYwhae -2

    Plasmid

    #198498

    Purpose

    lentiviral stable expression of mYwhae gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mYwhae (Ywhae Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Lenti-guide-puro mYwhae - 1

    Plasmid

    #198497

    Purpose

    lentiviral stable expression of mYwhae gRNA 1

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mYwhae (Ywhae Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. psiCHECK-NP

    Plasmid

    #196607

    Purpose

    Dual-Luciferase Reporter vector carrying a 425bp-long Influenza virus NP sequence in forward direction

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Insert

    nucleocapsid protein (NP) gene, partial
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSF-p15A-T7- YFP

    Plasmid

    #186568

    Purpose

    p15A ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

    Depositor

    Teresa de Diego Puente

    Article

    Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

    Insert

    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTE1069

    Plasmid

    #186629

    Purpose

    SCB-GFP E. coli reporter plasmid. Encodes repressor ScbR and ScbAp promoter upstream of GFP.

    Depositor

    Eriko Takano

    Article

    Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10

    Inserts

    scbR
    scbAp promoter
    

    Use

    Synthetic Biology

    Tags

    6xArg

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23100 and scbAp

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDR111_mannose_Bgal NLS

    Plasmid

    #188398

    Purpose

    Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Use

    Tags

    PhoD secretion peptide and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    D-mannose

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNB101

    Plasmid

    #169110

    Purpose

    Feedback-controlled AraC circuit

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    araC (araC Escherichia coli K12 MG1655)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNB200

    Plasmid

    #169111

    Purpose

    TESEC backbone for target expression

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    lacZ (lacZ Escherichia coli K12 MG1655)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBN216

    Plasmid

    #167963

    Purpose

    express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Y713E, Y715E, Y717E, Y718E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBN217

    Plasmid

    #167964

    Purpose

    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Y708E, Y713E, Y715E, Y717E, Y718E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBN262

    Plasmid

    #167967

    Purpose

    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Y708E, Y713E, Y715E, Y717E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBRC901

    Plasmid

    #167948

    Purpose

    express wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBN202

    Plasmid

    #167950

    Purpose

    express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    K540M, Y713E, Y715E, Y717E, Y718E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. 4LNDD-pCDNA3.1(+)

    Plasmid

    #172227

    Purpose

    Expresses human ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) in mammalian cells

    Depositor

    Joshua Rosenthal

    Article

    Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

    Insert

    Lambda N peptide - human ADAR2 Deaminase Domain
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pKP1693

    Plasmid

    #167595

    Purpose

    Express FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.

    Depositor

    Kathleen Postle

    Article

    Gresock et al J Bacteriol. 2017 Apr 25;199(10). pii: J

    Insert

    FepA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    His6 tag inserted after residue 393 in the mature…

    Promoter

    Available since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCMV-T7-null (BPK1077)

    Plasmid

    #171128

    Purpose

    Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

    Depositor

    Benjamin Kleinstiver

    Article

    Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

    Insert

    pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    pCMV and T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pKB238

    Plasmid

    #170033

    Purpose

    Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Neuropeptide Y (NPY Escherichia coli, Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pKB235

    Plasmid

    #170024

    Purpose

    Encodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    CCL20 (CCL20 Escherichia coli, Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. AlkB-D135G

    Plasmid

    #160434

    Purpose

    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly

    Depositor

    Tao Pan

    Article

    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

    Insert

    E. coli AlkB
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 135 to Glycine; deleted the…

    Promoter

    T7

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCDF-NtXNtS1HR1AtR2NtB2

    Plasmid

    #160890

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    PT7-Nt-rbcS-S1H
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    rbcS-SH1: T7, chaperones: T13

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCDF-NtXNtT4aR1AtR2NtB2

    Plasmid

    #160887

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    PT7-Nt-rbcS-T4a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcS-T4a: T7, chaperones: T10

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. SMT215

    Plasmid

    #134818

    Purpose

    SMT205 with DHFR-FLAG-tag, western blot

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. SMT101

    Plasmid

    #134815

    Purpose

    Dual expression of DHFR and TYMS, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge