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Showing: 2321 - 2360 of 160228 results

  2. lentiGuide_puro-P2A-mNeonGreen

    Plasmid
    #221464
    Purpose
    Lentiviral expression of an sgRNA with mNeonGreen fluorescent marker
    Depositor
    James Eshleman
    Insert
    mNeonGreen
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6, EF-1a
    Available since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    221464-plasmid-map-sequence-id-438191

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  3. pDONR221-SLC6A4_STOP

    Plasmid
    #161338
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC6A4 (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161338-plasmid-map-sequence-id-362127

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  4. IDG_KCNK7_OE_1

    Plasmid
    #161663
    Purpose
    Overexpresses KCNK7
    Depositor
    Mike McManus
    Insert
    KCNK7 (KCNK7 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    161663-plasmid-map-sequence-id-319647

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  8. GPR63-Tango

    Plasmid
    #66368
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR63 (GPR63 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pCAS9id

    Plasmid
    #141251
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141251-plasmid-map-sequence-id-308879

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  11. EBFP2-ER-5

    Plasmid
    #55240
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Use
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. pMG024 MBP-GSK3β-HA-His, GST_λPPase

    Plasmid
    #196182
    Purpose
    Co-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755
    Inserts
    GSK3B (GSK3B Human)
    λPPase
    Use
    Tags
    GST, HA, His6, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Tac
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196182-plasmid-map-sequence-id-383259

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  13. SARS-CoV Nucleocapsid

    Plasmid
    #154269
    Purpose
    Expresses myc-tagged nucleocapsid (N) from SARS-CoV (1) in mammalian cells
    Depositor
    Peter Klein
    Insert
    SARS-CoV Nucleocapsid
    Use
    Tags
    5X myc epitope
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    154269-plasmid-map-sequence-id-342917

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  14. pRDA_936

    Plasmid
    #216094
    Purpose
    Cas12a [EnAs] knockout targeting CD46, CD47, CD55, CD81, positive control
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Insert
    CD46, CD47, CD55, CD81 guides
    Use
    CRISPR and Lentiviral; Positive controls
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216094-plasmid-map-sequence-id-431378

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  15. pCMV14_Fibrillarin

    Plasmid
    #73061
    Purpose
    expression clone for human Fibrillarin with C-terminal 3xFLAG-tag
    Depositor
    Stefan Stamm
    Insert
    Fibrillarin (FBL Human)
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    73061-plasmid-map-sequence-id-134190

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  19. pEGPN-coBCL2L1

    Plasmid
    #188695
    Purpose
    express codon optimized BCL-XL
    Depositor
    Lewis Cantley
    Insert
    coBCL2L1 (BCL2L1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    codon optimized
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188695-plasmid-map-sequence-id-374882

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  23. pDL024_Tub-rb_IgG_LC

    Plasmid
    #193621
    Purpose
    α-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells.
    Depositor
    Jennifer DeLuca
    Article
    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:
    Insert
    Anti-Tubulin scFv light variable + rabbit constant chain
    Use
    Tags
    signal peptide
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193621-plasmid-map-sequence-id-380456

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  28. pDONR221-SLC7A3_STOP

    Plasmid
    #161467
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC7A3 (SLC7A3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161467-plasmid-map-sequence-id-355126

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  30. Col2.3deltaTK

    Plasmid
    #114273
    Purpose
    Conditional ablation of mature bone cells via ganciclovir exposure of cells expressing a truncated thymidine kinase.
    Depositor
    David Rowe
    Article
    Visnjic et al J Bone Miner Res. 2001 Dec;16(12):2222-3
    Insert
    Col2.3 promoter + deltaTK + BGH polyA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    2.3 kb rat type 1 collagen
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    114273-plasmid-map-sequence-id-220087

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  33. pDONR221-SLC27A4_STOP

    Plasmid
    #161449
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC27A4 (SLC27A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161449-plasmid-map-sequence-id-416481

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  34. mApple-Rab4a-7

    Plasmid
    #54943
    Purpose
    Localization: Ras GTPase, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Rab4a (RAB4A Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  36. pcDNA3-HCMVgO-sfGFP (TB40/E)

    Plasmid
    #136451
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFP
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    glycoprotein O (UL74 Human betaherpesvirus 5)
    Use
    Tags
    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136451-plasmid-map-sequence-id-271690

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  38. pTS2362_Tier3(SB)-rtTA-PuroR

    Plasmid
    #169658
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169658-plasmid-map-sequence-id-365631

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  39. mRaspberry-C1

    Plasmid
    #54659
    Purpose
    Localization: C1 Cloning Vector, Excitation: 598, Emission: 625
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mRaspberry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

    Plasmid
    #99694
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Synthetic, Mouse)
    Use
    AAV and CRISPR
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Jan. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    99694-plasmid-map-sequence-id-194587

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Showing: 2321 - 2360 of 160228 results