Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 5,933 results for: chia

Clear keywords
Showing: 2951 - 3000 of 5933 results

  1. pHL 1410

    Plasmid
    #53011
    Purpose
    pLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    DsrA sRNA
    ompC
    rpoS
    Use
    Synthetic Biology
    Tags
    T7noRBS (900bp) and gfp
    Expression
    Bacterial
    Mutation
    contains −150 to +30 bp relative to start codon a…
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. pHL1853

    Plasmid
    #53040
    Purpose
    PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA
    Depositor
    Han Lim
    Article
    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110
    Inserts
    GFP
    tetR
    csrA
    Use
    Synthetic Biology
    Tags
    LVA, RBS(st2), RBS(st3), and glgC leader
    Expression
    Bacterial
    Promoter
    pConNoHind and pLlac0-1
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pHL 990

    Plasmid
    #52992
    Purpose
    pLtetO-1:DsrA, pCon:RhyB, pLlacO-1:rpoS::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    DsrA sRNA
    RhyB sRNA (competitor)
    rpoS
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    includes −150 to +30 bp relative to start codon
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pHL 1022

    Plasmid
    #52996
    Purpose
    pLtetO-1:RhyB, pCon:OxyS, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    OxyS sRNA
    sodB
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pHL 762

    Plasmid
    #52990
    Purpose
    pLtetO-1:MicC, pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    MicC sRNA
    sodB
    ompC
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    −56 to +141 bp and −81 to +36 bp
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pHL 1020

    Plasmid
    #52995
    Purpose
    pLtetO-1:RhyB, pCon:DsrA, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    DsrA sRNA (competitor)
    sodB
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pHL 1019

    Plasmid
    #52994
    Purpose
    pLtetO-1:RhyB, pCon:MicC, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    MicC sRNA (Competitor)
    sodB
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pHL 1039

    Plasmid
    #53000
    Purpose
    pLtetO-1:DsrA, pCon:OxyS, pLlacO-1:rpoS::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    DsrA sRNA
    OxyS sRNA (competitor)
    rpoS
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    contains −150 to +30 bp relative to start codon
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pHL 1073

    Plasmid
    #53003
    Purpose
    pCon:ompC::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    mCherry
    GFP
    Use
    Synthetic Biology
    Tags
    ompC (target mRNA) and sodB (target mRNA)
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon a…
    Promoter
    pCon and pLlacO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pHL 1303

    Plasmid
    #53008
    Purpose
    pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    sodB
    ompC
    Use
    Synthetic Biology
    Tags
    GFP and mCherry
    Expression
    Bacterial
    Mutation
    contains -56 to +141 relative to start codon and …
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pREDCas9

    Plasmid
    #71541
    Purpose
    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    lambda red genes
    plasmid curing system
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71541-plasmid-map-sequence-id-333602

    Enlarge

  23. pFEndoNA2

    Plasmid
    #174667
    Purpose
    Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.
    Depositor
    Jukka Finne
    Article
    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149
    Insert
    GFP-endoNA2 fusion protein
    Tags
    Histag
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    174667-plasmid-map-sequence-id-343065

    Enlarge

  27. pAWPSG-Po-nCas9-BE

    Plasmid
    #195740
    Purpose
    Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Po (phoenol-inducible promoter)
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195740-plasmid-map-sequence-id-386428

    Enlarge

  29. pMJ922

    Plasmid
    #78312
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Tags
    HA-2xNLS-EGFP-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78312-plasmid-map-sequence-id-457381

    Enlarge

  31. Keasling-2337

    Plasmid
    #100168
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    lacIq-Ptrc-ADS
    Expression
    Bacterial
    Promoter
    trc
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100168-plasmid-map-sequence-id-193496

    Enlarge

  40. pORTMAGE-1

    Plasmid
    #72680
    Purpose
    Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Tags
    none
    Expression
    Bacterial
    Mutation
    lower strength RBS before mutL E32K allele than i…
    Available Since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only

  41. pTP-PCR C3

    Plasmid
    #189620
    Purpose
    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:
    Insert
    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Expression
    Bacterial and Yeast
    Mutation
    Removed repeat region, 10-kbp deletion, and minor…
    Available Since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    189620-plasmid-map-sequence-id-379033

    Enlarge

  42. pHL 1158

    Plasmid
    #53007
    Purpose
    pLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    DsrA sRNA
    ompC
    sodB
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    contains -56 to +141 relative to start codon and …
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  43. pJBEI-6411

    Plasmid
    #47050
    Purpose
    BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli
    Depositor
    Taek Soon Lee
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
    Use
    Synthetic Biology; Bglbrick
    Expression
    Bacterial
    Promoter
    Pbad
    Available Since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    47050-plasmid-map-sequence-id-67072

    Enlarge

Showing: 2951 - 3000 of 5933 results