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Plasmid#103994PurposePlasmid encoding mClover3DepositorInsertmClover3
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP
Plasmid#84254PurposeExpresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent markerDepositorInsertSa sgCXCR4-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-PhoCl-MBP
Plasmid#87689PurposeExpress PhoCl-MBP in E.coliDepositorInsertPhoCl fused to Maltose-Binding Protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-OmpT
Plasmid#68862Purposeconstruct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.DepositorInsertOmpT (ompT )
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pinducer20-dCas9-SunTag-2A-BFP
Plasmid#115779Purposetargeted DNA methylation toolDepositorInsertdCas9-SunTag-2A-BFP
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Paxillin-22
Plasmid#54219PurposeLocalization: Focal Adhesions, Excitation: 487, Emission: 509DepositorInsertPaxillin
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
M-SP-sgRNA
Plasmid#48671PurposeMammalian U6-driven sgRNA (SPm) targeting GTCCCCTCCACCCCACAGTGDepositorInsertsgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. pyogenes Cas9, hU6 promoter
UseCRISPRTagsExpressionMutationPromoterhU6Available sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMK394 (mAID-Nluc-Neo)
Plasmid#121195PurposeC-terminal taggingDepositorInsertmAID-Nluc-Neo
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
XRCC1-c003
Plasmid#173090PurposeExpression of recombinant fusion protein in E. coliDepositorInsertXRCC1 (XRCC1 Human)
UseTagsmCeruleanExpressionBacterialMutationE315-P406PromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-IT5
Plasmid#66869PurposeInntag 5: a small self-structured epitope aimed at innocuous protein taggingDepositorInsertInntag 5
UseTagsExpressionMutationPromoterAvailable sinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pReporter_5
Plasmid#60566PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_5
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRATIO2214
Plasmid#141303PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmCerulean and mScarlet-IExpressionPlantMutationPromoterUBQ10 promoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 I427V
Plasmid#81044Purposemammalian expression of LOV2 I427VDepositorInsertLOV I427V
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationI427VPromoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRATIO1267
Plasmid#141301PurposeGateway cloning compatible dual bioluminescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsgLUC and redLUCExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRATIO4214
Plasmid#141314PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmCerulean and mScarlet-IExpressionPlantMutationPromoterUBQ10 promoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 V416I
Plasmid#81045Purposemammalian expression of LOV V416IDepositorInsertLOV V416I
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationV416IPromoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GoldenGate
Plasmid#47388PurposeExpression plasmid for TALE proteins compatible with GoldenGate cloning systemDepositorTypeEmpty backboneUseTALENTagsFlag, HA, and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_8
Plasmid#60579Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_8
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PH
Plasmid#96948PurposeEGFP-PLC pleckstrin homology domain for CALI validationDepositorInsertPleckstrin homology domain
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
XW08
Plasmid#65832PurposePunc-4-QF-SL2-mcherry-3'UTR-unc-54DepositorInsertOR74A quinic acid utilization activator (qa-1F)
UseTagsmCherryExpressionWormMutationPromoterPunc-4Available sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_12
Plasmid#60583Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_12
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGTag Hygro EGFP nLAP +0
Plasmid#194305PurposeCRISPR/Cas9-mediated generic protein taggingDepositorInsertHygro-P2A-nLAP(EGFP)
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK376 (Hygro-P2A-mClover)
Plasmid#121187PurposeN-terminal taggingDepositorInsertHygro-P2A-mClover
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CytERM-dTomato
Plasmid#98834PurposeIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)DepositorInsertCytERM
UseTagsdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMito-iRFP713
Plasmid#45465DepositorInsertiRFP713
UseTagshuman cytochrome C oxidase subunit VIIIExpressionMammalianMutationT2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198…PromoterCMVAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSMVP-Cas9C-P2A-turboGFP
Plasmid#80935PurposePlasmid that expresses Cas9C in mammalian cells; co-expresses turboGFP.DepositorInsertCas9C
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
GatA_pET29b
Plasmid#215406PurposeExpression construct for GatA gene, codon optimized for expression in E. coliDepositorInsertGatA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNB648
Plasmid#60140PurposeNanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.DepositorInsertNLuc
UseTagsNuclear localization signal (NLS)ExpressionYeastMutationPromoterMET17prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-NLS-Vx3-mEGFP
Plasmid#35529DepositorInsertNLS-Vx3
UseRetroviralTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_13
Plasmid#60584Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_13
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB1-sfGFP
Plasmid#103983PurposePlasmid encoding sfGFPDepositorInsertsfGFP
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
5HT6-CFP-Venus(H148G)
Plasmid#47501PurposeFluorescent reporter for pH inside primary ciliaDepositorInsertVenus(H148G)
UseTags5HT6 and CFPExpressionMammalianMutationH148G mutationPromoterAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry
Plasmid#84249PurposeExpresses Sp sgSV40 gRNA with a mCherry fluorescent markerDepositorInsertSp sgSV40
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRATIO1112
Plasmid#141066PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmScarlet-I and mVenusExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG25-L-IFP-F[1] (clon 3)
Plasmid#52899PurposePlasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.DepositorInsertL-IFP-F[1]
UseVector for homologous recombinationTagsExpressionMutationPromoterTEF promoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG32-L-IFP-F[2] (clon 3)
Plasmid#52900PurposePlasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.DepositorInsertL-IFP-F[2]
UseVector for homologous recombinationTagsExpressionMutationPromoterTEF promoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_3
Plasmid#60575Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_3
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSY14-ptb
Plasmid#90490PurposepSY8 derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in ClostridiumDepositorInsertsceC
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_10
Plasmid#60581Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_10
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL690
Plasmid#47753PurposeExpresses dCas9-VP64 fusionDepositorInsertdCas9-VP64
UseCRISPRTags3x FLAG, NLS, and VP64ExpressionMammalianMutationD10A and H840A mutations in Cas9PromoterCMVAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only