We narrowed to 59,431 results for: plasmid
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Plasmid#16580DepositorAvailable sinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pX330-BbsI-PITCh
Plasmid#127875PurposeFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assemblyDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEP4 E02S CK2M EN2L
Plasmid#20924Purposeused in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseEpisomal/ebnaAvailable sinceMay 20, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FUW-tetO-hMYC
Plasmid#20723DepositorAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAc-sgRNA-Cas9
Plasmid#49330PurposeExpresses sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertsCas9
dU6-sgRNA
UseCRISPRTags3xFLAG and NLSExpressionInsectMutationHuman codon optimisedPromoterActin-5c and Drosophila U6Available sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
IFN-Beta_pGL3
Plasmid#102597PurposeIFN-beta promoter driving luciferase. Use in IFN reporter assay.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHuman IFN-Beta promoter (IFNB1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCX-cMyc
Plasmid#19772PurposeNon-integrating (episomal) expression of mouse c-MycDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertc-Myc (Myc Mouse)
ExpressionMammalianAvailable sinceJan. 5, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0046-EF1a-PspCas13b-NES-HIV
Plasmid#103862PurposeExpresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasmDepositorInsertPspCas13b
UseCRISPR and LentiviralTags3xHA and HIV NESExpressionMammalianAvailable sinceNov. 28, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pAAV-hSyn-DIO-EGFP
Plasmid#50457PurposeDouble floxed EGFP under the control of human synapsin promoterDepositorHas serviceAAV1, AAV2, AAV5, AAV8, AAV9, and AAV RetrogradeInsertEGFP
UseAAVTagsN/APromoterhuman Synapsin 1Available sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-gRNA
Plasmid#46759PurposegRNA cloning vector for in vitro transcription of target gRNADepositorInsertgRNA core
UseCRISPR; Zebrafish expressionPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPomyces-2
Plasmid#61737PurposeStreptomyces expression of codon-optimized Cas9 and custom gRNADepositorPublicationInsertssSpCas9
gRNA cassette
UseCRISPRExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL) and rpsL(XC)-BbsIAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.hSynapsin.SF-iGluSnFR.A184S
Plasmid#106174PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas serviceAAV1 and AAV9InsertSF-iGluSnFR.A184S
UseAAVMutationGltI: A184SPromoterhSynapsinAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pAAV-CaMKIIa-EGFP
Plasmid#50469PurposeEGFP under the control of CaMKIIa promoterDepositorHas serviceAAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, and AAV RetrogradeInsertEGFP
UseAAVTagsN/APromoterCaMKIIaAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_PGK_antiCD19_synNotch_Gal4VP64
Plasmid#79125PurposeLentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)DepositorInsertantiCD19_synNotch_Gal4VP64
UseLentiviralAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBS598 EF1alpha-EGFPcre
Plasmid#11923PurposeExpresses EGFP-Cre fusion under the control of the EF1alpha promoter; EGFP is human codon-optimized.DepositorPublicationAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-sgRNA EF1Alpha-puro-T2A-BFP
Plasmid#60955PurposeParental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralExpressionMammalianAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV.CamKII.GCaMP6s.WPRE.SV40
Plasmid#107790PurposeAAV expression of ultrasensitive protein calcium sensor from the CamKII promoterDepositorHas serviceAAV9InsertGCaMP6s
UseAAVExpressionMammalianPromoterCamKIIAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEP4 E02S EN2K
Plasmid#20925Purposeused in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseEpisomal/ebnaAvailable sinceMay 20, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p5xATF6-GL3
Plasmid#11976PurposeReporter gene with five ATF6 sites that is highly sensitive to ER stressDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert5xATF6 binding site
TagsLuc and c-fos promoterExpressionMammalianAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by