We narrowed to 55,915 results for: plasmid

1. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

   Plasmid

   #159912

   Purpose

   Mutagenesis of Kcnd3

   Depositor

   Larry Zweifel

   Insert

   Kcnd3 (Kcnd3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. KEAP1 (KEAP1A-c029)

   Plasmid

   #110247

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KEAP1 (KEAP1 Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7 - lacO

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

   Plasmid

   #105715

   Purpose

   In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.

   Depositor

   Rylan Larsen

   Has Service

   AAV5

   Insert

   synaptophysin-GCaMP6s
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   Ef1a, Human elongation factor-1 alpha

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. ANO10 (TMEM16KA-c001)

   Plasmid

   #110267

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TMEM16K (ANO10 Human)
   

   Tags

   TEV-His10-Flag

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pATF4.1.0-gDNA

   Plasmid

   #113772

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF4

   Depositor

   Kevin White

   Insert

   ATF4 (ATF4 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. JMJD1CA-c089

   Plasmid

   #79644

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   JMJD1C (JMJD1C Human)
   

   Tags

   6x His and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICE URA3

   Plasmid

   #122882

   Purpose

   URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Available Since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ACE2 g2

   Plasmid

   #153012

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

   Depositor

   Jason Sheltzer

   Insert

   ACE2 gRNA (ACE2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJUNB.1.0-gDNA

   Plasmid

   #112409

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor JUNB

   Depositor

   Kevin White

   Insert

   JUNB (JUNB Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-6His-SUMO-Nsp14

    Plasmid

    #154956

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
    

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pELK3.1.0-gDNA

    Plasmid

    #112418

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELK3

    Depositor

    Kevin White

    Insert

    ELK3 (ELK3 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. ACE2 g3

    Plasmid

    #153013

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

    Depositor

    Jason Sheltzer

    Insert

    ACE2 (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. GALTA-c001

    Plasmid

    #79645

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GALT (GALT Human)
    

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTFE3.1.0-gDNA

    Plasmid

    #112473

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFE3

    Depositor

    Kevin White

    Insert

    TFE3 (TFE3 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICE HIS3

    Plasmid

    #122877

    Purpose

    HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Expression

    Yeast

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICE TRP1

    Plasmid

    #122881

    Purpose

    TRP1 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Expression

    Yeast

    Available Since

    Oct. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET22-6His-SUMO-Nsp10

    Plasmid

    #154960

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pICE LYS2

    Plasmid

    #122879

    Purpose

    LYS2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Expression

    Yeast

    Available Since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICE LEU2

    Plasmid

    #122878

    Purpose

    LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Expression

    Yeast

    Available Since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pERF.1.0-gDNA

    Plasmid

    #112395

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ERF

    Depositor

    Kevin White

    Insert

    ERF (ERF Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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