We narrowed to 14,310 results for: eng
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Plasmid#11154PurposeMammalian expression vector for expression of GFP (EF1a promoter)DepositorAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pXR002: EF1a-dCasRx-2A-EGFP
Plasmid#109050PurposeCatalytically inactive dCasRx with 2A-EGFPDepositorInsertdCasRx
UseCRISPR and LentiviralTags2A-eGFP, HA, and NLSExpressionMammalianMutationR239A/H244A/R858A/H863AAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pL-CRISPR.EFS.GFP
Plasmid#57818PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGPromoterEFS and hU6Available sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CROPseq-Guide-Puro
Plasmid#86708PurposeMakes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Human sgRNA library Brunello in lentiGuide-Puro
Pooled library#73178PurposeHuman sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controlsDepositorHas serviceLentiviral PrepExpressionMammalianUseCRISPR and LentiviralAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BPK1520
Plasmid#65777PurposeHuman expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNADepositorInsertSpCas9 gRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MoClo Plant Parts III: Transformation & Genome Engineering Kit
Plasmid kit#1000000236PurposeCollection of MoClo Level 0 parts for plant transformation, including regulatory elements, coding sequences, basic CRISPR parts, and parts for modular geminiviral replicon assembly.DepositorApplicationCloning and Synthetic BiologyVector typePlant ExpressionCloning typeGolden Gate (MoClo)Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmU6-gRNA
Plasmid#53187PurposeExpresses the S. pyogenes sgRNA from the mouse U6 promoterDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromotermouse U6Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA_AAVS1-T2
Plasmid#41818PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineeringDepositorInsertgRNA_AAVS1-T2
UseCRISPRExpressionMammalianAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiCRISPRv2 blast
Plasmid#98293PurposeVariant of lentiCRISPRv2 that confers blasticidin S resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralPromoterEF-1aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
phH1-gRNA
Plasmid#53186PurposeExpresses the S. pyogenes sgRNA from the H1 promoterDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterhuman H1Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-EF1a-IRES-Puro
Plasmid#85132Purposelentiviral backboneDepositorTypeEmpty backboneUseLentiviralPromoterEF1aAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
M58 pPET28b-TEV-full-length zebrafish beta catenin
Plasmid#17199DepositorAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330A-1x2
Plasmid#58766PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-BbsI-chiRNA
Plasmid#45946PurposePlasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.DepositorPublicationInsertU6-BbsI-chiRNA
UseCRISPRExpressionInsectPromoterDm-snRNA:U6:96AbAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ph7SK-gRNA
Plasmid#53189PurposeExpresses the S. pyogenes sgRNA from the 7SK promoterDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterhuman 7SKAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9n(BB) (PX460)
Plasmid#48873PurposeCas9n (D10A nickase mutant) from S. pyogenesDepositorInserthSpCas9n
UseCRISPRTags3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-T7pol
Plasmid#59926PurposeExpression vector for T7 RNA polymerase geneDepositorInsertT7 RNA polymerase
ExpressionMammalianPromoterCAGAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD3-dU6:3gRNA
Plasmid#49410Purposeexpression of gRNA under control of the Drosophila U6:3 promoterDepositorInsertdU6-3:gRNA
UseCRISPRExpressionInsectPromoterdU6-3Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST-HisMBP
Plasmid#11085DepositorTypeEmpty backboneTags6xHis and MBPExpressionBacterialPromoterTac (lactose/IPTG inducible)Available sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by