We narrowed to 21,390 results for: eng
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Plasmid#67935PurposeMammalian expression of mouse vinculin with GFP fusionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVinculin (Vcl Mouse)
TagsEGFPExpressionMammalianMutationL159P in vinculinPromoterCMVAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET 28 full-length human PARP1
Plasmid#192635PurposeExpresses human PARP1 with an N-terminal His-tagDepositorAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUNO mouse CD274 + 3'UTR WT full length
Plasmid#107012PurposeExpresses murine PD-L1 with the 3'UTRDepositorInsertCD274 (Cd274 Mouse)
ExpressionMammalianAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q109
Plasmid#111730PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ109 (polyQ repeat)PromoterCMV and P10Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q19
Plasmid#111741PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ19 (polyQ repeat)PromoterCMV and P10Available sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q23
Plasmid#111723PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ23 (polyQ repeat)PromoterCMV and P10Available sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9n(BB)-2A-GFP (PX461)
Plasmid#48140PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthSpCas9n
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q79
Plasmid#111729PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ79 (polyQ repeat)PromoterCMV and P10Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9n(BB)-2A-GFP (PX461)
Plasmid#48140PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthSpCas9n
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Psicheck2 COLIA1 full length 3'UTR
Plasmid#26993DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCOL1A1 Full length 3' UTR (COL1A1 Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMammalianAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)
Plasmid#42335PurposeA human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTagsHAExpressionMammalianMutationD10A nickase-converting mutationAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-SENP8 (full length))
Plasmid#16361DepositorInserthuman SENP8 full length (SENP8 Human)
TagsHisExpressionBacterialMutationhuman SENP8 full length, see Depositor CommentsAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)
Plasmid#42335PurposeA human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTagsHAExpressionMammalianMutationD10A nickase-converting mutationAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9(BB)-2A-Puro (PX459)
Plasmid#48139PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.DepositorInserthSpCas9-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9(BB)-2A-Puro (PX459)
Plasmid#48139PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.DepositorInserthSpCas9-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA_Cloning Vector
Plasmid#41824PurposeAn empty gRNA expression vector, used to create a gRNA to a specific sequence.DepositorTypeEmpty backboneUseCRISPRTagsAflII site for Gibson AssemblyExpressionMammalianAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINK-OTUD1 (full-length, aa 1-481)
Plasmid#61405PurposeExpresses human OTUD1 (full-length) in E. coli.DepositorAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINK-OTUD2 (full-length, aa 1-348)
Plasmid#61409PurposeExpresses human OTUD2 (full-length) in E. coli.DepositorAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
gRNA_Cloning Vector
Plasmid#41824PurposeAn empty gRNA expression vector, used to create a gRNA to a specific sequence.DepositorTypeEmpty backboneUseCRISPRTagsAflII site for Gibson AssemblyExpressionMammalianAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX-4T1 human PP2A Aα full length
Plasmid#132634Purposeexpresses human PP2A Aα full length in E. coliDepositorInsertPP2A Aα (PPP2R1A Human)
ExpressionBacterialAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only