165,637 results

1. pCN34e-ktrA-yfp

   Plasmid

   #227640

   Purpose

   c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

   Depositor

   Angelika Grundling

   Article

   Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

   Insert

   ktrA-yfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFP.R268

   Plasmid

   #138180

   Purpose

   Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(LNR).gp41-8.(SAV)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   P(araBAD) and iPC

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HMBP-3C-STAR.66-285

   Plasmid

   #100094

   Purpose

   expresses MBP-tagged STARD1 protein domain (residues 66-285)

   Depositor

   Nikolai Sluchanko

   Article

   Sluchanko et al Protein Expr Purif. 2016 Mar;119:27-35.

   Insert

   STAR protein (STAR Human)
   

   Use

   Tags

   His-tagged MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKM491-Flag-His

   Plasmid

   #109282

   Purpose

   ORBIT integrating plasmid for C-terminal tagging with FLAG-His tag

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   attB-Flag-His
   

   Use

   Orbit integrating plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Syn-ChrimsonR-tdT (AAV5)

   Viral Prep

   #59171-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Promoter

   Syn

   Tags

   tdTomato

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

6. STK11 gRNA (BRDN0001146880)

   Plasmid

   #75912

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human STK11

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   STK11 (STK11 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. SI10-Brevican

   Plasmid

   #46300

   Depositor

   Gavin Wright

   Article

   Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

   Insert

   Brevican mouse monoclonal antibody (bcan Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pGal-BE3

   Plasmid

   #172409

   Purpose

   Yeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoter

   Depositor

   Leonid Kruglyak

   Article

   Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

   Insert

   BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GALL

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-Halo,IRES-eGFP

   Plasmid

   #164519

   Purpose

   All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)

   Depositor

   Mitchell Guttman

   Article

   Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPBpuro-ErbB1

    Plasmid

    #197358

    Purpose

    Encoding human ErbB1.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS_4xhyb PylT A41AA C55A CRFR1 95TAG 263TAA-HA

    Plasmid

    #154781

    Purpose

    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAG 263TAA, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    CRFR1 (Crhr1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    95TAG 263TAA in CRFR1-HA, hybrid PylT with A41AA …

    Promoter

    EF1

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLYS5-SDHB-Flag

    Plasmid

    #50055

    Purpose

    Expression of human SDHB in mammalian cells

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    SDHB (SDHB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMOD_B2520

    Plasmid

    #91077

    Purpose

    Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    OsU6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. mApple-Rab7a-7

    Plasmid

    #54945

    Purpose

    Localization: Ras GTPase, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    Rab7a (RAB7A Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCVL.SSA TLR (Sce)

    Plasmid

    #45576

    Purpose

    Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    5' iRFP arm
    eGFP with I-Sce I TS
    +3 mCherry
    3' iRFP arm
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Sce I TS from 163-185bp, truncated 25 …

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV pCAG-FLEX-EGFP-WPRE (AAV2)

    Viral Prep

    #51502-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Promoter

    CAG

    Tags

    EGFP (Cre-dependent)

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pSIQmate

    Plasmid

    #216240

    Purpose

    All-in One IQ-Cumate transgene switch, empty vector

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+ phiC31 integrase

    Plasmid

    #216237

    Purpose

    For the overexpression of phiC31 integrase

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Insert

    integrase from phage φC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF0461

    Plasmid

    #142267

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CREB5 (CREB5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Active Wnt11-V5

    Plasmid

    #43824

    Depositor

    Xi He

    Article

    MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

    Insert

    human WNT11 (WNT11 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHAGE mNeonGreen-Core (HBc from HBV) IRES puro

    Plasmid

    #122202

    Purpose

    Plasmid coding for mNeonGreen-Core protein (from HBV AYW) and a puromycin resistance in a lentiviral backbone.

    Depositor

    Raphael Gaudin

    Article

    Hallez et al Nat Microbiol. 2019 Apr 1. pii: 10.1038/

    Inserts

    HBc from HBV AYW
    mNeonGreen
    

    Use

    Tags

    GGSGGSGGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDONR223-DCAMKL2

    Plasmid

    #23604

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    DCAMKL2 (DCLK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pELxG4

    Plasmid

    #172570

    Purpose

    Inducible gene expression, pET origin, LuxR activator, PLuxB promoter, GFPmut3, Carbenicillin Resistance Marker.

    Depositor

    Christopher Reisch

    Insert

    LuxR-GFPmut3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLuxB

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pQE30 PTBP1 isoform1

    Plasmid

    #108591

    Purpose

    Recombinant His tagged protein production

    Depositor

    Chris Smith

    Article

    Wollerton et al RNA. 2001 Jun;7(6):819-32.

    Insert

    PTBP1 isoform 1 (PTBP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    isoform 1 (isoform c)

    Promoter

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMAH-POLY-eRF1(E55D)

    Plasmid

    #157925

    Purpose

    Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

    Depositor

    Huiwang Ai

    Article

    Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

    Insert

    POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-anti-mCherry(LaM8) PAGER(TF)

    Plasmid

    #229999

    Purpose

    Expresses anti-mCherry PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    IL2SP-Aro6-LaM8-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    V360A/R361A on KORD

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCHA1.1-MFSD12-V5

    Plasmid

    #149508

    Purpose

    Stable expression of human MFSD12 with recoded nucleotide sequence.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Codon Optimized Sequence

    Promoter

    Ubc

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GFP-JIP3

    Plasmid

    #164624

    Purpose

    Expresses JIP3 with a GFP tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    JIP3 (MAPK8IP3 Human)
    

    Use

    Tags

    cycle 3 GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA-ER-ZapCY1

    Plasmid

    #36321

    Depositor

    Amy Palmer

    Article

    Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

    Insert

    ER-ZapCY1 high affinity Zn(II) sensor
    

    Use

    Tags

    KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

    Plasmid

    #92121

    Purpose

    CRISPR-Cas9 based efficient genome editing in S. aureus

    Depositor

    Sheng Yang

    Article

    Liu et al Acta Biochim Biophys Sin (Shanghai). 201

    Insert

    Cas9-Pxyltet-sgRNA-pj23119
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge