167,910 results

1. pJBL7062

   Plasmid

   #167231

   Purpose

   cell-free cascaded copper sensor

   Depositor

   Julius Lucks

   Article

   Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

   Insert

   pCue-AKSIRV
   

   Use

   Synthetic Biology

   Tags

   6X His

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJBL7044

   Plasmid

   #167215

   Purpose

   overexpression of PbrR in E. coli for cell-free lead sensing

   Depositor

   Julius Lucks

   Article

   Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

   Insert

   PbrR
   

   Expression

   Bacterial

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJBL7061

   Plasmid

   #167230

   Purpose

   cell-free cascaded lead sensor

   Depositor

   Julius Lucks

   Article

   Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

   Insert

   pPbr-AKSIRV
   

   Use

   Synthetic Biology

   Tags

   6X His

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-DIO-ChR2(H134R)-eYFP (AAV PHP.eB)

   Viral Prep

   #127090-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-DIO-ChR2(H134R)-eYFP (#127090). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-ChR2(H134R)-eYFP plasmid DNA. CAG-driven, Cre-dependent expression of humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Viviana Gradinaru

   Article

   Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

   Promoter

   CAG

   Tags

   EYFP (Cre-dependent)

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

5. pDS220

   Plasmid

   #34980

   Purpose

   Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   ePDZb–mCherry
   

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. 4xCSL-luciferase

   Plasmid

   #41726

   Depositor

   Raphael Kopan

   Article

   Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

   Insert

   4xCSL binding sites
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Contains a multimerized high affinity CSL binding…

   Available Since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJBL7056

   Plasmid

   #167225

   Purpose

   orthogonal T7 RNAP sfGFP reporter

   Depositor

   Julius Lucks

   Article

   Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

   Insert

   pAKSIRV-sfGFP
   

   Use

   Synthetic Biology

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJBL7085

   Plasmid

   #167249

   Purpose

   orthogonal mRFP reporter output for cell-free expression

   Depositor

   Julius Lucks

   Article

   Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

   Insert

   pAKSIRV-mRFP1
   

   Use

   Synthetic Biology

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTYB1-eIF4A

   Plasmid

   #37225

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Insert

   eIF4A
   

   Tags

   intein

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pJBL7079

    Plasmid

    #167244

    Purpose

    cell-free lac sensor with GFP output

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pLac-sfGFP
    

    Use

    Synthetic Biology

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJBL7080

    Plasmid

    #167245

    Purpose

    cell-free lac sensor with RFP output

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pLac-mRFP1
    

    Use

    Synthetic Biology

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJBL7081

    Plasmid

    #167246

    Purpose

    cell-free lac sensor with Mango output

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pLac-Mango
    

    Use

    Synthetic Biology

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJBL7082

    Plasmid

    #167247

    Purpose

    cell-free lac sensor with XylE output

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pLac-XylE
    

    Use

    Synthetic Biology

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJBL7086

    Plasmid

    #167250

    Purpose

    orthogonal Mango reporter output for cell-free expression

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pAKSIRV-Mango
    

    Use

    Synthetic Biology

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pR6K-GFP-CASTIF

    Plasmid

    #199653

    Purpose

    R6K plasmid encoding a type I-F CAST but no CRISPR array

    Depositor

    IIya Finkelstein

    Insert

    CAST I-F systems
    

    Expression

    Bacterial

    Promoter

    J23119 promoter

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pClodANL-FGF2

    Plasmid

    #107871

    Purpose

    Bacterial expression of FGF2-NanoLuc

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Insert

    FGF2
    

    Use

    Luciferase

    Tags

    NanoLuc

    Expression

    Bacterial

    Mutation

    wt sequence

    Promoter

    pro D

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3/F-tractin=mStayGold

    Plasmid

    #212019

    Purpose

    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    F-tractin
    

    Tags

    mStayGold

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. haA1.pBABEpu

    Plasmid

    #21813

    Depositor

    David Ron

    Article

    Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

    Insert

    GADD34 C-term
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV5)

    Viral Prep

    #55645-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent channelrhodopsin-EYFP expression for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Promoter

    Syn

    Tags

    EYFP (Cre- and Flp-dependent)

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

20. pLenti-CMV-Puro-AAVR-FLAG

    Plasmid

    #166716

    Purpose

    Vector expressing AAVR (KIAA0319L) with a C-terminal Flag tag

    Depositor

    Jan Carette

    Article

    Pillay et al Nature. 2016 Feb 4;530(7588):108-12. doi

    Insert

    AAVR-FLAG (KIAA0319L Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pENTR5'_ubi:loxP-EGFP-loxP

    Plasmid

    #27322

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    ubiquitin:loxP-EGFP-loxP
    

    Use

    Multisite gateway 5' entry vector

    Available Since

    April 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAVone-AAV9-CMV-eGFP

    Plasmid

    #230928

    Purpose

    pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.

    Depositor

    Qizhao Wang

    Article

    Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

    Insert

    eGFP
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. HyPer7.2DAAO-NLS

    Plasmid

    #168302

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the nucleus

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. LentiGuide_DKO

    Plasmid

    #183193

    Purpose

    Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

    Depositor

    Lang Li

    Article

    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6, mU6

    Available Since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLenti PGK Blast DEST (w524-1)

    Plasmid

    #19065

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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26. lenti-mTagBFP2

    Plasmid

    #216126

    Purpose

    Lentiviral vector for expressing mTagBFP2.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-mTagBFP2-WPRE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. hNav1.8_pcDNA3.1

    Plasmid

    #182847

    Purpose

    Expresses hNav1.8 in mammalian cells

    Depositor

    Paul DeCaen

    Article

    McCollum et al Cell Rep. 2022 Aug 23;40(8):111248. doi:

    Insert

    SCN10A (SCN10A Human)
    

    Expression

    Mammalian

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCas9

    Plasmid

    #42876

    Purpose

    Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    tracr/Cas9
    

    Use

    CRISPR; E.coli

    Expression

    Bacterial

    Available Since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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29. AP1muA-Halo-ALFA-polyA-G418 HR

    Plasmid

    #229681

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    Halo, ALFA

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. 35S-eGFP-nosT

    Plasmid

    #80127

    Purpose

    Transient expression vector for eGFP in plants

    Depositor

    Yutaka Kodama

    Article

    Fujii et al Plant Biotech. 2015; 32(1): 81-87.

    Type

    Empty backbone

    Tags

    eGFP

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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