We narrowed to 18,992 results for: Met;
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modRNAc0-Cas9
Plasmid#170180PurposeIVT template plasmid for making modified Cas9 RNADepositorInsertCas9
UseIvt template plasmidPromoterT7 promoterAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS
Plasmid#79373PurposeRecruits VPR to a compatible Cas9 protein for transcriptional activationDepositorInsertGCN4-sfGFP-VPR
UseCRISPRExpressionMammalianAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEos4a-C1
Plasmid#54810PurposeLocalization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581DepositorTypeEmpty backboneTagsmEos4aExpressionMammalianAvailable SinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTE101
Plasmid#59396PurposeMethyloBrick cloning vector, no promoter; KmRDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoternoneAvailable SinceDec. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pTEF1p-CYC1t
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailable SinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS
Plasmid#79372PurposeRecruits p65-hsf1 to a compatible Cas9 protein for transcriptional activationDepositorInsertGCN4-sfGFP-p65-hsf1
UseCRISPRExpressionMammalianAvailable SinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GoldenGate-VP64
Plasmid#47389PurposeExpression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning systemDepositorTypeEmpty backboneUseTALENTagsFLAG, HA, and SV40 NLSExpressionMammalianPromoterCMVAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-PhoCl-mCherry-myc
Plasmid#87692PurposeExpress PhoCl-mCherry with C-term myc tag in mammalian cellsDepositorInsertPhoCl-mCherry-myc
Tagsmyc tagExpressionMammalianAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-citrate
Plasmid#225251PurposeBacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrateDepositorInsertqmTQ2-citrate
Tags6XHis, T7 tag, XpressTM tagExpressionBacterialPromoterT7 promoterAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct_mScarlet-H_N1
Plasmid#85055PurposeIn vivo visualization of actin cytoskeleton (can be used for colocalization studies)DepositorInsertLifeAct
ExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioD
Plasmid#65350Purposesysthesis of vioD gene in the violacein pathwayDepositorInsertvioD
UseSynthetic BiologyAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gi-c001
Plasmid#173078PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-335-5p-Decoy.hPGK.GFP.WPRE
Plasmid#46612DepositorInsertmiR-335-5p Decoy
UseLentiviralPromoterhU6Available SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
mMaroon-PAK-mMaroon
Plasmid#84361PurposeFRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroonDepositorInsertmMaroon-PAK-mMaroon
TagsmMaroon flanking PAK domain on N and C terminusExpressionMammalianPromoterCMVAvailable SinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMBA328
Plasmid#214748PurposeMedium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RBS(22821)-mcherry
ExpressionBacterialAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioB
Plasmid#65348Purposesysthesis of vioB gene in the violacein pathwayDepositorInsertvioB
UseSynthetic BiologyAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH_SBP-mCherry-Ecadherin
Plasmid#65291PurposeEcadherin reporter only (no hook) (RUSH system)DepositorInsertEcadherin
UseLentiviralTagsIL-2 Signal Sequence and Streptavidin Binding Pro…PromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SMVP-Cas9N-U6-gRNA P2
Plasmid#80944PurposeExpresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 bindingDepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianAvailable SinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-92a-Decoy.hPGK.GFP.WPRE
Plasmid#46638DepositorInsertmiR-92a Decoy
UseLentiviralPromoterhU6Available SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCytERM_mScarlet-H_N1
Plasmid#85067PurposeIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)DepositorInsertCytERM
TagsmScarlet-HExpressionMammalianMutationaa1-29PromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP
Plasmid#31477DepositorInsertSFFV HA NLS Y2 T2A mTagBFP
UseLentiviralTagsHA and NLSMutationF13Y + S111Y on I-AniI = Y2 "reo" (res…Available SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
mMaroon-RTK-mMaroon
Plasmid#84359PurposeFRET acceptor for RhoA donor, flanked with the far-red FP mMaroonDepositorInsertmMaroon-Rtk--mMaroon
TagsmMaroon flanking Rtk domain on N and C terminusExpressionMammalianPromoterCMVAvailable SinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRNA-U6-let7 sponge
Plasmid#35664DepositorInsertU6 let-7sponge
PromoterU6Available SinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOVSD
Plasmid#81033PurposeMammalian expression of the super-dark state of LOV2DepositorInsertLOVSD
TagsmCherryExpressionBacterial, Insect, and Mamm…MutationC450A, L514K, G528A, L531E, N538EAvailable SinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLI-N-GFP-2xFKBP-loxP(K13)
Plasmid#85792PurposeP. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLIDepositorInsertKelch13
UseCre/Lox; P. falciparum genomic integrationTags2xFKBP, GFPPromoternoneAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDS277
Plasmid#34973PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastMutationV529NAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMK401 (Hygro-P2A-mAID-Nluc)
Plasmid#121196PurposeN-terminal taggingDepositorInsertHygro-P2A-mAID-Nluc
UseCRISPRAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CL7-LbCas12aD156R
Plasmid#164659PurposeBacterial expression of mutated LbCas12aD156R using CL7/Im7DepositorInsertLbCas12a-D156R
TagsThioredoxin, CL7, SUMOExpressionBacterialMutationchanged Aspartic Acid 156 to ArgininePromoterT7Available SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
SBP-EGFP-Ecadherin_puromycin
Plasmid#65292PurposeEcadherin reporter only (no hook) (RUSH system)DepositorInsertEcadherin
TagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1
Plasmid#81133Purposemammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domainDepositorAvailable SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTE103
Plasmid#59398PurposeMethyloBrick expression vector, fumC promoter; TcRDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPfumCAvailable SinceDec. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1201
Plasmid#44483DepositorInsertMinimal Mos1, unc-119, peel-1, MCS
UseMultiple cloning site vectorAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
M-SPn-VP64
Plasmid#48674PurposeMammalian SP-VP64 nuclease-null Cas9 activator expression, human optimizedDepositorInsertCas9-VP64, nuclease-null
UseCRISPRTagsNLS and VP64Mutationnuclease-nullPromoterCMVAvailable SinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_NES_C1
Plasmid#85062PurposeIn vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)DepositorInsertNES
ExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
mRuby2-MyosinIIA-C-18
Plasmid#55906PurposeLocalization: Cytoskeleton, Excitation: 559, Emission: 600DepositorInsertMyosinIIA
TagsmRuby2ExpressionMammalianPromoterCMVAvailable SinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGH156_MS2-AID-Hygro
Plasmid#85415Purposelentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable markerDepositorInsertMS2-AID and Hygromycin resistance
UseCRISPR and LentiviralExpressionMammalianAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTE104
Plasmid#59399PurposeMethyloBrick expression vector, coxB promoter; TcRDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPcoxBAvailable SinceDec. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VP64
Plasmid#78901PurposeExpresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cellsDepositorInsertdCas9-VP64
UseCRISPRExpressionInsectPromoterpActin (Drosophila)Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1272
Plasmid#44486DepositorInsertMinimal Mos1, MCS
UseMultiple cloning site vectorAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only
