-
-
C078
Plasmid#174347PurposeTemplate for making mRNA for overexpressing frog ePAB (C-FLAG) in frog oocytesDepositorInsertePAB
UseIn vitro transcription templateTagsFLAGExpressionMutation13 silent mutations: G243A, A273G, G339A, C345G, …PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
C233
Plasmid#174349PurposeTemplate for making mRNA for overexpressing frog PABPC1 M161A mutant (C-FLAG) in frog oocytesDepositorInsertPABPC1 (M161A) (pabpc1.S Frog)
UseIn vitro transcription templateTagsFLAGExpressionMutationM161APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
KJ740(DE3)
Bacterial Strain#179486PurposeKJ740(DE3) is an OmpC-, OmpF-deletion strain of E. coli that allows for T7 promoter-based expressionDepositorBacterial ResistanceTetracycline (chromosome, tn10) (10 μg/ml); streptomycin/spectinomycin (chromosome) (50 μg/ml)SpeciesEscherichia coliAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCBC-DT1T2
Plasmid#50590PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT1, U626t plus, U629p, T2
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
sgCtr- LentiCRISPRv2
Plasmid#107402PurposeLentiviral expression of Cas9 and a control gRNADepositorInsertcontrol gRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pediculus humanus corporis PINK1 (115 - 575)
Plasmid#110750PurposeBacterial expression of PhPINK1 (biochemistry)DepositorInsertPhum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
UseTagsN-His6-GST-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pET16b-Trx
Plasmid#36944DepositorInsertThioredoxin 1 (TXN Human)
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTP264
Plasmid#149334PurposeBacterial expression of Biotin ligase BirADepositorInsertBiotin ligase BirA
UseTagsHis14 and bdNEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
pET15b-RvLEAMshort
Plasmid#218122PurposeExpresses truncated RvLEAM protein under IPTG induction in bacterial host cells.DepositorInsertRvLEAMshort
UseTags6xHISExpressionBacterialMutationTruncated form (58-181aa)PromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis-hTG2
Plasmid#100719PurposeExpression of human transglutaminase 2 in E.coliDepositorInsertTGM2 (TGM2 Human)
UseTagsHisExpressionBacterialMutationsilent mutation Arg580 (AGG--CGT) to enhance expr…PromoterT7Available sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
PAmCherry1-C1-P4C
Plasmid#202680PurposePhotoactivatable lipid biosensorDepositorInsertPAmCherry1: L. pneumophila SidC (608-773) (sidC Legionella pneumophila)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-GGA2
Plasmid#199172PurposeExpresses GFP-GGA2 fusion in mammalian cellsDepositorInsertGGA2 (GGA2 Human)
UseTagsEGFPExpressionMammalianMutationAla 23 Thr substitution; silent substitution in c…PromoterCMVAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cas9-His
Plasmid#98158PurposePurification of Cas9 proteinDepositorInsertNLS-Cas9-NLS
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pscALPS- SIV-MAC251 vpx
Plasmid#115810PurposeEncodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance proteinDepositorInsertSIVMAC251 Vpx
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW103ParsR-Amp30C
Plasmid#78638PurposepBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-tDepositorInsert30hrpR-30hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX_311-KRAB-dCas9
Plasmid#96918Purposefor CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121DepositorInsertdCas9
UseLentiviralTagsKRABExpressionMammalianMutationdCas9PromoterEF1aAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
Plasmid#60910PurposedCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag systemDepositorInsertdCas9
UseCRISPR and LentiviralTags2x NLS, HA, and NLSExpressionMammalianMutationPromoterdSV40 PromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
COM-epegRNA-Dnmt1
Plasmid#211376PurposeDnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP productionDepositorInsertCOM-epegRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SENP6m
Plasmid#18716DepositorInsertFLAG-SENP6m (SENP6 Human)
UseTagsFLAGExpressionMammalianMutationC1030APromoterAvailable sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Cytochrome C
Plasmid#41181DepositorInsertCytochrome C (CYCS Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-EGFP
Plasmid#52343PurposeAAVS1 donor vector for inducible gene expressionDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
Plasmid#60904PurposeThe plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64DepositorInsertscFv-GCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z07
Plasmid#124790PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeonGreenDepositorInsertmNeonGreen
UsePcr templateTagsmNeonGreenExpressionMutationPromoterNoneAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P23
Plasmid#120034PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with HaloDepositorInsertHalo
UsePcr templateTagsHaloExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P13
Plasmid#120024PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherryDepositorInsertmCherry
UsePcr templateTagsmCherryExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-NPM WT
Plasmid#17578DepositorInsertNPM (NPM1 Human)
UseTagsEGFP and FlagExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOZ-N-FH MERIT40
Plasmid#27498DepositorInsertMERIT40 (BABAM1 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOZ-N-FH KIAA0157
Plasmid#27499DepositorInsertKIAA0157 (ABRAXAS2 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
Plasmid#60903PurposeExpressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activationDepositorInsertCas9 dead
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA hEZH2
Plasmid#24230PurposeMammalian expression of human EZH2 with HA tagDepositorInsertEZH2 (EZH2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
MS2-epegRNA-Dnmt1
Plasmid#211372PurposeDnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP productionDepositorInsertMS2-epegRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-hnRNP-H
Plasmid#23020DepositorInserthnRNP-H (HNRNPH1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Nup35
Plasmid#87342PurposeTo express human Nup35 in mammalian cellsDepositorInsertnucleoporin 35 (NUP35 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a MISTIC
Plasmid#85999Purposebacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tagDepositorInsertN/A
UseTagsHIS6 and MISTICExpressionBacterialMutationcodon optimzed for bacterial expressionPromoterT7Available sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAV1-mCherry
Plasmid#27705DepositorInsertcaveolin-1 (CAV1 Canis lupus familiaris)
UseTagsmonomeric CherryExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
EGFP-C3-beta-spectrin
Plasmid#202685Purposefluorescent protein tagged beta-spectrinDepositorInsertEGFP:SPTB(1-2364) (SPTB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
