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GFAP-Cre

Plasmid

#24704

Depositor

Michael Sofroniew

Article

Bush et al Neuron. 1999 Jun . 23(2):297-308.

Insert

Cre

Expression

Mammalian

Promoter

GFAP (glial fibrillary acidic protein)

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoB-NAD

Plasmid

#193824

Purpose

CMV overexpression of ChemoB-NAD in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB-NAD

Expression

Mammalian

Mutation

EBFP2:N39Y, A206K, T225R; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pETM6-vioABECD

Plasmid

#66535

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Expression

Bacterial

Promoter

Consensus T7

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-mArg1 base promoter -31/-2365

Plasmid

#34570

Depositor

Peter Murray

Article

Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

Insert

Arg1 base promoter -31/-2365 (Arg1 Mouse)

Use

Luciferase

Tags

Firefly Luciferase

Available Since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only
pEBG-hUBP43

Plasmid

#12455

Depositor

Dong-Er Zhang

Article

Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

Insert

UBP43 (USP18 Human)

Tags

GST

Expression

Mammalian

Mutation

pEBG vector is from Bruce Mayer, may be different…

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pBITE-EGFRvIII-F265-OKT3

Plasmid

#190679

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F265 clone)

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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AviTag-SpyTag003-MBP

Plasmid

#184227

Purpose

Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

AviTag-SpyTag003-MBP

Tags

AviTag, His6, SpyTag003, and Thrombin cleavage si…

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMSC168

Plasmid

#216350

Purpose

xhNup35-Nb1t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup35-Nb1t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PKCA D463N

Plasmid

#195203

Purpose

Mammalian expression vector for GFP tagged PKCA. Kinase-inactive mutant

Depositor

David Bryant, Peter Parker

Article

Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

Insert

PRKCA (PRKCA Human)

Expression

Mammalian

Mutation

D463N

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-mRuby3-2A-Cre

Plasmid

#203844

Purpose

Flp-dependent Cre and mRuby3 expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Cre

Use

AAV

Tags

mRuby3-2A

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP

Plasmid

#158963

Purpose

Cre-dependet Cas9-GFP expression

Depositor

Michael Brand

Article

Kesavan et al Development. 2020 Jun 8;147(11). pii: de

Inserts

hsp70l promoter
floxed Non-FP cassette
Cas9-GFP

Use

CRISPR and Cre/Lox

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z02

Plasmid

#120045

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TagBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TagBFP

Use

Pcr template

Tags

TagBFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 Rap2A

Plasmid

#19311

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RAP2A, member of RAS oncogene family (RAP2A Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pMSC115

Plasmid

#216356

Purpose

xNup93-Nb2t Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup93-Nb2t

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMRE137

Plasmid

#118491

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGEL850

Plasmid

#214250

Purpose

Expression TadCBEa in rice cells

Depositor

Yong Zhang

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Type

Empty backbone

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRJPaph-bjGFP

Plasmid

#67032

Purpose

Plasmid for Paph-bjGFP integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
bjGFP

Use

Conjugative bacterial vector

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-PKAKTRClover

Plasmid

#59146

Purpose

ORF encoding PKA KTR mClover flanked by gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA Kinase Translocation Reporter (PRKACA Mouse, Human)

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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Ccas9LacZ

Plasmid

#113083

Purpose

Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.

Depositor

Tibor Vellai

Article

Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

Type

Empty backbone

Use

CRISPR

Expression

Worm

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-BFP

Plasmid

#214363

Purpose

Expression of the Blue Fluorescent Protein

Depositor

Bertrand Collet

Article

Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

Insert

BFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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miniTn7(Gm)PA1/04/03-eyfp-a

Plasmid

#111620

Purpose

eYFP Fluorescent tagging of bacteria at their Tn7-site

Depositor

Tim Tolker-Nielsen

Article

Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

Insert

PA1/04/03-eyfp

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDG04163

Plasmid

#216373

Purpose

xhNup214-Nb1t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup214-Nb1t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-scSUMO

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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TB:7xE-Box:MiniP

Plasmid

#133880

Purpose

7 copies of the E-box motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

7 copies of the E-box motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE130

Plasmid

#118484

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Expression

Bacterial

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRE133

Plasmid

#118487

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAdEasy Flag GCN5

Plasmid

#14106

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

GCN5 (KAT2A Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

E189G and K195N

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-G12-CheRiff-T2A-mCherry

Plasmid

#170275

Purpose

CheRiff-T2A-mCherry construct expression in mammlian cells. pAAV vector. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

CheRiff-T2A-mCherry

Use

AAV

Promoter

11xUAS with TATA-box minimal promoter

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pUB-cSypHer

Plasmid

#84733

Purpose

Expresses SypHer in plant cells-biosensor for pH

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

SypHer

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT-ChemoG-NAD

Plasmid

#193821

Purpose

CMV overexpression of ChemoG-NAD in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-NAD

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Bmp4

Plasmid

#163588

Purpose

Expresses BMP-4 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp4 (Bmp4 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5 Smad1-HA

Plasmid

#14956

Depositor

Joan Massague

Article

Liu et al Nature. 1996 Jun 13. 381(6583):620-3.

Insert

Smad1 (SMAD1 Human)

Tags

HA

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF CREB M1

Plasmid

#22969

Depositor

Marc Montminy

Article

Du et al Mol Cell Biol. 2000 Jun . 20(12):4320-7.

Insert

CREB (Creb1 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pREP3-TEV-NLS

Plasmid

#21146

Depositor

Stephen Kearsey

Article

Yang et al BMC Mol Biol. 2005 Jun 7;6:13. doi: 10.1

Insert

TEV protease (TEVgp1 tobacco etch virus)

Use

S. pombe

Tags

2xNLS, NLS, and myc

Expression

Yeast

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Dead-Lapronic/pcDNA3.1(-)

Plasmid

#140753

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio, Negative Control

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Dead-Lapronic

Expression

Mammalian

Mutation

Changed Histidine 92 to Alanine

Promoter

CMV

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF11-wElectra1

Plasmid

#184932

Purpose

Codon optimized blue fluorescent protein Electra1 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1

Expression

Worm

Promoter

tag-168

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSC234

Plasmid

#216357

Purpose

xhNup93-Nb3t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup93-Nb3t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRJPaph-mChe

Plasmid

#67034

Purpose

Plasmid for Paph-mCherry integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
mCherry

Use

Conjugative bacterial vector

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE80l BCB

Plasmid

#196472

Purpose

Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher

Depositor

Fei Sun

Article

Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

Insert

BCB

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFYF1327 EGFP Site#2

Plasmid

#47512

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
YF-RhoA(CA)

Plasmid

#20153

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-RhoA(CA) (RHOA Human)

Expression

Mammalian

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
hRIP1 HA GFP wt

Plasmid

#41396

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP1 (RIPK1 Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

E96G

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pRJPaph-sYFP2

Plasmid

#67033

Purpose

Plasmid for Paph-sYFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
sYFP2

Use

Conjugative bacterial vector

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRE152

Plasmid

#118502

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Expression

Bacterial

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL852

Plasmid

#214252

Purpose

Expression TadCBEd_V106W in rice cells

Depositor

Yong Zhang

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Type

Empty backbone

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-Z05

Plasmid

#120048

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-puro-Flag-mMcl-1

Plasmid

#32982

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Retroviral

Tags

3XFlag

Expression

Mammalian

Promoter

LTR

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pMaCTag-23

Plasmid

#120002

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with Halo

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Halo

Use

Pcr template

Tags

Halo

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-CMV10-hTti1

Plasmid

#30213

Depositor

Noboru Mizushima

Article

Kaizuka et al J Biol Chem. 2010 Jun 25. 285(26):20109-

Insert

Tel2 interacting protein 1 (TTI1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pRK5-HA-SH3BP4

Plasmid

#46891

Purpose

expresses HA tagged SH3BP4 in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 (SH3BP4 Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
TREER-Actin

Plasmid

#208330

Purpose

TREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER-Actin

Tags

TREER

Expression

Mammalian

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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