159,557 results

1. pMRX-IP-HaloTag7-LC3

   Plasmid

   #184899

   Purpose

   Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Use

   Tags

   HaloTag7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-HA-FbxO11-pcDNA3.1-

   Plasmid

   #52501

   Purpose

   expresses human FbxO11 in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   FbxO11 (FBXO11 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-Foxo1 (pCMV5)

   Plasmid

   #12142

   Depositor

   Domenico Accili

   Article

   Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

   Insert

   Foxo1 (Foxo1 Mouse)
   

   Use

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Wild-type

   Promoter

   CMV

   Available since

   Aug. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tet-on circRNA Expression Vector

   Plasmid

   #92351

   Purpose

   The vector can ef_ciently produce a circRNA (circmCherry) from a split EGFP gene after Dox treatment.

   Depositor

   Ling-Ling Chen

   Article

   Zhang et al Cell Rep. 2016 Apr 19;15(3):611-24. doi:

   Insert

   mCherry and split EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTet

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5_mEGFP-NUP98-DDX10

   Plasmid

   #237640

   Purpose

   For overexpression of mEGFP-NUP98-DDX10

   Depositor

   Denes Hnisz

   Insert

   NUP98-DDX10 (NUP98, DDX10 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tet-pLKO-puro-Scrambled

   Plasmid

   #47541

   Purpose

   Tet inducible scrambled shRNA.

   Depositor

   Charles Rudin

   Article

   Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

   Insert

   Scrambled shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-link-ymNeongreen-SpHis5

   Plasmid

   #125704

   Purpose

   Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selection

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeonGreen
   

   Use

   Tags

   ymNeonGreen

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. N-Cadherin-EGFP

   Plasmid

   #18870

   Depositor

   Valeri Vasioukhin

   Article

   Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

   Insert

   N-Cadherin (Cdh2 Mouse)
   

   Use

   Tags

   EGFP, Kanamycin, and N-Cadherin

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT3-Neo-EF1a-GFP

   Plasmid

   #69134

   Purpose

   Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

   Depositor

   Martin Bonamino

   Article

   Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

   Type

   Empty backbone

   Use

   Sleeping beauty transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U3MSCV

   Available since

   Jan. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-21b(+)-Is-PETase-W185A

    Plasmid

    #112204

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    

    Use

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    W185A

    Promoter

    N/A

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCF1148

    Plasmid

    #222330

    Purpose

    Fluorescent reporter plasmid for Veillonella

    Depositor

    J Christopher Fenno

    Article

    Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

    Insert

    bs2 gene with Veillonella parvula SKV38 mdh promoter
    

    Use

    E. coli-veillonella shuttle plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Veillonella parvula SKV38 mdh promoter

    Available since

    Oct. 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pmTurquoise2-T2A-Venus(L68V)

    Plasmid

    #60494

    Purpose

    Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    mTurquoise2-T2A-mVenus(L68V)
    

    Use

    Tags

    T2A peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. phDLX2-N174

    Plasmid

    #60860

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    DLX2 (DLX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-hNINJ1-GFP

    Plasmid

    #208779

    Purpose

    Inducibly expresses human NINJ1 tagged with GFP

    Depositor

    Petr Broz

    Article

    Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

    Insert

    NINJ1 (NINJ1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3TS-RfxCas13d-NLS-HA

    Plasmid

    #141321

    Purpose

    Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos, Ariel Bazzini

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d (NLS-RfxCas13d-NLS)
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AbVec2.0-IGHG1

    Plasmid

    #80795

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHCMV-VSVgSS-pAG-TM

    Plasmid

    #207318

    Purpose

    Functional incorporation of protein AG (pAG) fusion protein into envelope of virions

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    protein AG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmRFP_a_tubulin_IRES_puro2b

    Plasmid

    #21041

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Use

    Tags

    pmRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. CRY2olig-FUS

    Plasmid

    #218921

    Purpose

    Expression of CRY2olig-mCherry fused to FUS

    Depositor

    Tatyana Shelkovnikova

    Insert

    CRY2olig-mCherry tagged FUS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. AiP12828 - pAAV-AiE0456m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2828)

    Plasmid

    #224006

    Purpose

    AiE0456m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. ptf-Galectin3

    Plasmid

    #64149

    Purpose

    Expression in mammalian cells

    Depositor

    Tamotsu Yoshimori

    Article

    Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

    Insert

    Galectin3 (LGALS3 Human)
    

    Use

    Tags

    EGFP and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EMTB-mNeonGreen

    Plasmid

    #137802

    Purpose

    Visualization of microtubules

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    Ensconsin microtubule binding domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pscAAV-CAG-GFP

    Plasmid

    #83279

    Purpose

    recombinant AAV vector packaging self-complementary GFP under the CAG promoter

    Depositor

    Mark Kay

    Article

    Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

    Insert

    enhanced green fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. DDX41-GFP

    Plasmid

    #175494

    Purpose

    Total RNA was isolated from TF-1 cells and converted to cDNA using SuperScript III (Invitrogen). The full DDX41 coding sequence was cloned into pcDNA3.1 (Invitrogen) with an EGFP c-terminal insert.

    Depositor

    Evan Braunstein

    Article

    Li et al Haematologica. 2016 Jun;101(6):e228-31.

    Insert

    DDX41 (DDX41 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HIV-1-GFP ΔEnv

    Plasmid

    #217437

    Purpose

    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu.

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLVX-ATF4 mScarlet NLS

    Plasmid

    #115969

    Purpose

    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    activating transcription factor 4 (ATF4 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    HA tag, c-myc NLS, and mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-hSyn-DIO-mCherry

    Plasmid

    #50459

    Purpose

    Double floxed mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    mCherry
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIB184 (EM)

    Plasmid

    #90194

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-CHRNA7-YFP

    Plasmid

    #62630

    Purpose

    mammalian expression of human Alpha7 (CHRNA7) with YFP fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    hAlpha7 (CHRNA7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    YFP inserted in M3-M4 loop

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR-UCOE-EF1a-Zim3-dCas9-BFP

    Plasmid

    #188775

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9-mTagBFP2
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge