159,258 results

1. VN-Sph1

   Plasmid

   #89755

   Purpose

   expresses human Synphilin fused to the N-terminal part of Venus

   Depositor

   Tiago Outeiro

   Article

   Dominguez-Meijide et al Neurobiol Dis. 2021 Apr;151:105256. doi:

   Insert

   SNCAIP (SNCAIP Human)
   

   Use

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. EC67844

   Plasmid

   #209514

   Purpose

   Cloning in complete guide expression cassettes, with Cas9

   Depositor

   Wendy Harwood, Cristobal Uauy

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIB185

   Plasmid

   #90196

   Purpose

   E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

   Depositor

   Indranil Biswas

   Article

   Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

   Type

   Empty backbone

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-Luciferase-IRES-mCerulean

   Plasmid

   #75022

   Purpose

   Encodes the expression of Luciferase and mCerulean

   Depositor

   Saradhi Mallampati

   Insert

   Luciferase
   

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pLV-SspB-HaloTag-p63DH

   Plasmid

   #176129

   Purpose

   Heterodimerization with iLID, RhoA activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   RHOGEF p63 (ARHGEF25 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF1788

   Plasmid

   #143847

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TCERG1 (TCERG1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP_WT-talin1 (1-2541)

   Plasmid

   #166112

   Purpose

   For expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.

   Depositor

   Vesa Hytönen

   Article

   Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

   Insert

   WT-Talin-1 (Tln1 Mouse)
   

   Use

   Cmv promoter

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-Klf4

   Plasmid

   #13370

   Purpose

   Retroviral expression of mouse Klf4

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   Kruppel-like factor 4 (gut) (Klf4 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

   Plasmid

   #101060

   Purpose

   AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Has Service

   AAV1

   Insert

   CaMPARI2
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Expression

   Mutation

   Promoter

   hsyn (synapsin-1)

   Available since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2- mRuby-Rab35

    Plasmid

    #194338

    Purpose

    Expresses mRuby Rab35 in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Insert

    Rab35 (RAB35 Human)
    

    Use

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. R702-X80-635: His6-tev-Hs.NF1opt (1-2818) isoform 2

    Plasmid

    #159575

    Purpose

    Baculovirus protein expression of His6-tev-Hs.NF1opt (1-2818) isoform 2

    Depositor

    Dominic Esposito

    Article

    Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

    Insert

    His6-tev-Hs.NF1opt (1-2818) isoform 2 (NF1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI neo-hEST2-HA

    Plasmid

    #1782

    Depositor

    Bob Weinberg

    Article

    Meyerson et al Cell 1997 Aug 22;90(4):785-95.

    Insert

    hTERT (TERT Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Clone is catalytically active but is dead in vivo.

    Promoter

    Available since

    July 10, 2005

    Availability

    Academic Institutions and Nonprofits only

13. amilCP chromoprotein promoter-less

    Plasmid

    #118008

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    RBS, amilCP
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiV_Neo_SIK3_CR

    Plasmid

    #108104

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    change guanine 501 to cytosine (silent mutation)

    Promoter

    EFS promoter

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMIY-Arg1

    Plasmid

    #34580

    Depositor

    Peter Murray

    Insert

    Arg1 (Arg1 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pCellFree_G03 CIRBP

    Plasmid

    #67055

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CIRBP (CIRBP Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. LentiV_Neo_SIK3_CR_K95M

    Plasmid

    #108106

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    change guanine 501 to cytosine (silent mutation),…

    Promoter

    EFS promoter

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.2 GW delCMV

    Plasmid

    #29496

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCV-NINJ1

    Plasmid

    #201179

    Purpose

    Expression of NINJ1 gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

    Insert

    Ninjurin 1 (Ninj1 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQCXIH-HA NEDD4L

    Plasmid

    #27028

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    NEDD4L (NEDD4L Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pMK438 (Stag-3FLAG-P2A-Hygro)

    Plasmid

    #168233

    Purpose

    C-terminal tagging with Stag-3FLAG

    Depositor

    Masato Kanemaki

    Article

    Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

    Insert

    Stag-3FLAG-P2A-Hygro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. DYRK1A gRNA (BRDN0001145031)

    Plasmid

    #76755

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DYRK1A

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DYRK1A (DYRK1A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. PB_TetON_mCherry_P2AT2A_GFP-nb-KS

    Plasmid

    #238240

    Purpose

    For integration of mCherry_P2AT2A_GFP-nb-KS

    Depositor

    Denes Hnisz

    Insert

    mCherry_P2AT2A_GFP-nb-KS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRK5_mCherry_P2AT2A_GFP-nb-KS-FtoA

    Plasmid

    #238234

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb-KS-FtoA

    Depositor

    Denes Hnisz

    Insert

    mCherry_P2AT2A_GFP-nb-KS-FtoA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRK5_mCherry_P2AT2A_GFP-nb-KS

    Plasmid

    #238233

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb-KS

    Depositor

    Denes Hnisz

    Insert

    mCherry_P2AT2A_GFP-nb-KS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PB_TetON_mCherry_P2AT2A_GFP-nb-KS-FtoA

    Plasmid

    #238241

    Purpose

    For integration of mCherry_P2AT2A_GFP-nb-KS-FtoA

    Depositor

    Denes Hnisz

    Insert

    mCherry_P2AT2A_GFP-nb-KS-FtoA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRK5_EGFP-BRD4-NUT

    Plasmid

    #238254

    Purpose

    For overexpression of EGFP-BRD4-NUT

    Depositor

    Denes Hnisz

    Insert

    EGFP-BRD4-NUT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPB.DEST-ITGB1(WT)-mRuby2

    Plasmid

    #215448

    Purpose

    Piggybac expression vector with integrin beta1 tagged with mRuby2

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Transposon-based stable expression

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Silent mutations added to disrupt shRNA binding a…

    Promoter

    CAG

    Available since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pVeBtA1

    Plasmid

    #238986

    Purpose

    Mammalian expression of bovine arrestin-1 with Venus N-terminal fusion

    Depositor

    Vsevolod Gurevich

    Article

    Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

    Insert

    arrestin-1 (SAG Bovine)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA -CLCN3-3xFlag

    Plasmid

    #225506

    Purpose

    CLCN3 expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

    Insert

    CLCN3 (CLCN3 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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