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Bacterial Expression

29,729 results

Showing: 5201 - 5250 of 29729 results

  1. pHJL1

    Plasmid
    #199376
    Purpose
    Encodes dual cistronic cytosolic GCaMP6s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    GCaMP6s::SL2::mScarlet-I
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations
    Promoter
    Available since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199376-plasmid-map-sequence-id-390952

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  15. pETSG

    Plasmid
    #159418
    Purpose
    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli
    Depositor
    Dianfan Li
    Article
    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:
    Insert
    thermostable GFP (TGP)
    Use
    Tags
    polyhistidine (poly-His)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    159418-plasmid-map-sequence-id-321533

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  28. pPbcas13b

    Plasmid
    #89906
    Purpose
    Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
    Depositor
    Feng Zhang
    Article
    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.
    Insert
    Cas13b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89906-plasmid-map-sequence-id-178224

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  29. KRASB

    Plasmid
    #25153
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KRASb:M1-K169 (KRAS Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Q61H
    Promoter
    Available since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pQdC12a

    Plasmid
    #191356
    Purpose
    Clostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expression
    Depositor
    Andrew Tolonen
    Article
    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-
    Inserts
    PGusA2-TetO2/1-dCas12a (LbCpf1)
    miniPthl-tetR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PGus2A-TetO2/1 and miniPthl
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    191356-plasmid-map-sequence-id-378049

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  36. pRelA'

    Plasmid
    #175595
    Purpose
    Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Catalytic domain of E.coli relA gene.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Only the catalytic domain of the enzyme is used (…
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175595-plasmid-map-sequence-id-348284

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  37. pV1F

    Plasmid
    #205084
    Purpose
    Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.
    Depositor
    Suomeng Dong
    Article
    Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.
    Insert
    AtVIP1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    agorbacterium virB operon promoter
    Available since
    Aug. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    205084-plasmid-map-sequence-id-405743

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  39. pJE53

    Plasmid
    #194153
    Purpose
    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.
    Depositor
    Edward Geisinger
    Article
    Bai et al J Bacteriol. 2021 May 20;203(12):e005652
    Insert
    sgRNA with mrfp control guide sequence
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    194153-plasmid-map-sequence-id-382435

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  42. pDEST-N2H-N1

    Plasmid
    #125547
    Purpose
    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment1 attached to n-terminus of Gatew…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125547-plasmid-map-sequence-id-254706

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  44. pAH121

    Plasmid
    #164901
    Purpose
    The pAH121 plasmid [Haldimann and Wanner 2001] is the phi21 helper plasmid necessary for genomic integration of the pCYMR expression plasmid.
    Depositor
    Keith Shearwin
    Article
    Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612
    Insert
    phi21 Integrase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    164901-plasmid-map-sequence-id-327647

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  46. pNIC-CH

    Plasmid
    #26117
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Opher Gileadi
    Type
    Empty backbone
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    Sept. 17, 2010
    Availability
    Academic Institutions and Nonprofits only
    26117-plasmid-map-sequence-id-12262

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  48. pBAD-GINKO1

    Plasmid
    #113111
    Purpose
    Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1
    Depositor
    Robert Campbell
    Article
    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1
    Insert
    GINKO1
    Use
    Tags
    6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    113111-plasmid-map-sequence-id-216972

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Showing: 5201 - 5250 of 29729 results