Skip to main content
Addgene

29,740 results

Showing: 5401 - 5450 of 29740 results
  1. pSW002-Pc-TorA(sp)-mTurquoise2

    Plasmid
    #111254
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter followed by E. coli TorA signal peptide in frame with mTurquoise2 (codon optimized for P. fluorescens); export to periplasm
    Depositor
    Insert
    TorA(sp)-mTurquoise2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mTurquoise2 is codon optimized for expression in …
    Promoter
    Pc
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pDipA-TEV-His12

    Plasmid
    #137042
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12
    Depositor
    Insert
    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    Use
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. EPHA5

    Plasmid
    #25144
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    epha5.653.939 (EPHA5 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. pTR47m4

    Plasmid
    #104068
    Purpose
    Luciferase Reporter for Formaldehyde Sensing
    Depositor
    Inserts
    xluxAB
    frmR
    frm Promoter m4
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    23r and 25th nucleotides in sequence mutated from…
    Promoter
    Available since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pGHUC w-ERBIN (2-hybrid prey plasmid)

    Plasmid
    #128163
    Purpose
    UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).
    Depositor
    Insert
    ERBIN PDZ domain (ERBIN Synthetic)
    Use
    Synthetic Biology
    Tags
    FLAG and Omega subunit of RNA polymerase
    Expression
    Bacterial
    Mutation
    Promoter
    UV5
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. pOPS0377

    Plasmid
    #115509
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFP
    Depositor
    Insert
    sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Constitutive promoter pNeo pOGG001
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. XXBLM (XXBLMA-c075)

    Plasmid
    #98223
    Purpose
    Vector for periplasmic expression of Lama antibodies with PelA leader and C-terminal His6 tag
    Insert
    XXBLMA (BLM Human)
    Use
    Tags
    His-6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pTXB1-CII

    Plasmid
    #51566
    Purpose
    Expression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Insert
    cII
    Use
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
  9. pBK086

    Plasmid
    #183145
    Purpose
    pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expression
    Depositor
    Insert
    MapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
    Use
    Tags
    6xHis and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pM1s3AsR_TS-C7

    Plasmid
    #137945
    Purpose
    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression
    Depositor
    Insert
    mRFP1
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pMal-Strep

    Plasmid
    #209345
    Purpose
    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagII
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. pET32a-DEST

    Plasmid
    #84652
    Purpose
    E. coli expression vector derived from pET32 with Gateway cassette.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    His, S tag, and Trx Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
  13. pMM441

    Plasmid
    #61271
    Purpose
    Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.
    Depositor
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 5401 - 5450 of 29740 results