29,740 results

1. pDEST17-UnaG

   Plasmid

   #203485

   Purpose

   For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

   Insert

   UnaG
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET_BGL1

   Plasmid

   #101910

   Purpose

   Expresses a β-glucosidase gene from Thermomicrobium roseum in E. coli under the control of the pET system.

   Depositor

   Steve Shih

   Article

   Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944

   Insert

   β-glucosidase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7+LacO

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pENTR1A-SynTetOff-GFP

   Plasmid

   #190230

   Purpose

   Gateway entry vector, high-level expression of EGFP in neuronal cells

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETM11-ACS

   Plasmid

   #108943

   Purpose

   heterologous expression of ACS of E. coli in E. coli

   Depositor

   Frank Schulz

   Article

   Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

   Insert

   Acetyl-CoA synthetase (ACS)
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

   Plasmid

   #216283

   Purpose

   Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SpyCatcher003-TEVs-SpyTag003DA
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-MBP-Geo_st

   Plasmid

   #87700

   Purpose

   Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP

   Depositor

   Jennifer Doudna

   Insert

   GeoCas9
   

   Use

   Tags

   10xHis-MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT7NT*-Bri2 113-231 R221E

   Plasmid

   #138134

   Purpose

   Expresseds human Bri2 BRICHOS R221E mutant in E. coli

   Depositor

   Jan Johansson

   Article

   Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

   Insert

   human Bri2 BRICHOS R221E (ITM2B Human)
   

   Use

   Tags

   NT* tag derived from spider silk proteins

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pH6HTC_IGF2BP2a-Halo

   Plasmid

   #175309

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   IGF2BP2 (IGF2BP2 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Dead Streptavidin-SpyCatcher

   Plasmid

   #59547

   Purpose

   pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

   Insert

   Dead-dloop-Streptavidin-SpyCatcher
   

   Use

   Tags

   N-terminally shortened SpyCatcher

   Expression

   Bacterial

   Mutation

   PolyAsp in L3/4 loop for ion-exchange

   Promoter

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pOPINHALO7

    Plasmid

    #41117

    Depositor

    Ray Owens

    Article

    Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLJSRSF7

    Plasmid

    #64693

    Purpose

    His-MBP-Smt3-NpuDnaEN102-CBD

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Type

    Empty backbone

    Use

    Tags

    CBD, HisTag, MBP, NpuDnaE-N102, and Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pWVR5

    Plasmid

    #167509

    Purpose

    Broad-Host range expression vector, pSa origin, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    VanRAM-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PVanCC

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GST-Hsp90 C(626-732)

    Plasmid

    #22483

    Depositor

    William Sessa

    Article

    Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

    Insert

    Hsp90alpha C domain (HSP90AA2P Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST17-UbE2G2

    Plasmid

    #15791

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2G2 (UBE2G2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST-GST-FBW7 alpha

    Plasmid

    #197456

    Purpose

    The plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.

    Depositor

    Omar Khan

    Article

    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

    Insert

    FBW7 (FBXW7 Human)
    

    Use

    In vitro translation

    Tags

    GST

    Expression

    Bacterial

    Mutation

    See depositor comments below

    Promoter

    T7

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TDP-43_CTD_del321-343

    Plasmid

    #98676

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with a deletion of AA 321-343

    Depositor

    Nicolas Fawzi

    Article

    Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    del321-343

    Promoter

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSCB2-sgRNA

    Plasmid

    #129463

    Purpose

    Template plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    pgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)

    Plasmid

    #48283

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1.Gα13-LgB106

    Plasmid

    #134364

    Purpose

    Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gα13-LgB106 (GNA13 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-SARS-CoV-2 NSP13

    Plasmid

    #176492

    Purpose

    Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

    Depositor

    Xiaodong Cheng

    Article

    White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

    Insert

    SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    I570 from SARS-CoV NSP13 was mutated to V to conv…

    Promoter

    T7

    Available since

    Jan. 10, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pET21b-Caspase-3(D3A,V266E)

    Plasmid

    #90091

    Purpose

    Expresses caspase D3A and V266E mutant in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D175A D28A D9A V266E

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. IGF2BP1_2xRRM-4xKH_pGEX

    Plasmid

    #135122

    Purpose

    Recombinant expression of IGF2BP1 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    IGF2BP1 (IGF2BP1 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJKR-L-tetR

    Plasmid

    #62562

    Purpose

    Tetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOKD4

    Plasmid

    #17214

    Depositor

    Hao Wu

    Article

    Dzivenu et al Protein Expr Purif. 2004 Nov . 38(1):1-8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pR1G108CP20X

    Plasmid

    #85086

    Purpose

    Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    G108C in PCNA1

    Promoter

    T7 promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAB50

    Plasmid

    #101678

    Purpose

    mCherry under T7 promoter control

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    mCherry under T7 promoter control
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBJ1

    Plasmid

    #167130

    Purpose

    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Frr promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAD/HisD-PATagRFP

    Plasmid

    #31945

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

    Insert

    PATagRFP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

    Promoter

    araB

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pProEx Rab1A(S20C) CL

    Plasmid

    #224272

    Purpose

    Protein purification of Rab1A (S20C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rab1A (RAB1A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S20C

    Promoter

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLY071f

    Plasmid

    #227942

    Purpose

    SICLOPPS precursor for cyclo-CRCVFSG

    Depositor

    Tina Wang

    Article

    Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

    Insert

    Evolved Ssp SICLOPPS-CRCVFSG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Evolved Ssp split inteins with multiple mutations…

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. T. aq RNA Polymerase

    Plasmid

    #215349

    Purpose

    Express T. aq RNA Polymerase subunits in E. coli cells

    Depositor

    Julius Lucks

    Article

    Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

    Insert

    RNA polymerase
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. SSH-pSUMO-α-COPI-WD40

    Plasmid

    #208341

    Purpose

    Bacterial expression plasmid for Schizosaccharomyces pombe α-COPI-WD40

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    N-terminal WD40 domain of yeast α-COPI (1-327) with substitutions (Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Phe197Lys)
    

    Use

    Tags

    His-tag, SUMO-tag, and Strep-tag

    Expression

    Bacterial

    Mutation

    Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Ph…

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SPLICSFAST-NU-MT-P2A

    Plasmid

    #214898

    Purpose

    Expresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-NU-MT-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBad33_ISEc41_reRNA

    Plasmid

    #205341

    Purpose

    Bacterial expression of ISEc41 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISEc41 TnpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBad33_ISEc46_reRNA

    Plasmid

    #205343

    Purpose

    Bacterial expression of ISEc46 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISEc46 TnpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBad33_ISNsp2_reRNA

    Plasmid

    #205350

    Purpose

    Bacterial expression of ISNsp2 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISNsp2 TnpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHH0103_SKAP2-1/1

    Plasmid

    #91447

    Purpose

    Protein expression and purification of human SH3 domain construct SKAP2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SKAP2-1/1 (SKAP2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET-His6-Sumo-CfaC-MBP

    Plasmid

    #126018

    Purpose

    Bacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)

    Depositor

    Thomas Muir

    Insert

    His6-Sumo-CfaC-MBP
    

    Use

    Tags

    6xHis-SUMO and HA-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET-28a (+)-hTFIIB

    Plasmid

    #171084

    Purpose

    Expresses human TFIIB in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TFIIB

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    TF2B (GTF2B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTK-enhNC1.1_M3-MCS-2A-eGFP-SV40pA

    Plasmid

    #110203

    Purpose

    Recipient vector for Cranial Neural Crest-specific expression of protein of interest

    Depositor

    Tatjana Sauka-Spengler

    Insert

    enhNC1.1_M3-TF_MCS-2A-eGFP-SV40pA
    

    Use

    Tags

    eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBS human IRS-1

    Plasmid

    #11359

    Depositor

    Ronald Kahn

    Article

    Araki et al Diabetes. 1993 Jul . 42(7):1041-54.

    Insert

    IRS-1 (IRS1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSN007

    Plasmid

    #102440

    Purpose

    For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

    Depositor

    George Church, Alejandro Chavez

    Article

    Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

    Insert

    7SK paired gRNA insert
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pTHSSe_52

    Plasmid

    #109250

    Purpose

    ColE1 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23105

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCORE-UH

    Plasmid

    #72239

    Purpose

    Contains the CORE cassette with KlURA3 and hyg markers

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    KlURA3
    hyg
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pDule-Mb haloTyrRS C6

    Plasmid

    #160377

    Purpose

    C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

    Depositor

    Ryan Mehl

    Article

    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

    Insert

    C6 HaloTyrosine tRNA synthatase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    L270S, Y271L, N311G, C313T

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. pWaldo-GFPe-SH1446

    Plasmid

    #112507

    Purpose

    Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

    Depositor

    Simon Newstead

    Article

    Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

    Insert

    POT family transporter
    

    Use

    Tags

    C terminal TEV GFP octa-His affinity tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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47. mCarmine in pRSETB

    Plasmid

    #109484

    Purpose

    bacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

    Depositor

    Oliver Griesbeck

    Article

    Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

    Insert

    mCarmine
    

    Use

    Tags

    N-terminal histidine tag in vector

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. pHH0103_RANBP1_Ran-BP1

    Plasmid

    #109872

    Purpose

    Protein expression and purification of RANBP1_Ran-BP1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RANBP1_Ran-BP1 (RANBP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. TGT-His

    Plasmid

    #138201

    Purpose

    Expresses E. Coli TGT with a C-terminal His Tag

    Depositor

    Neal Devaraj

    Article

    Busby et al Methods Enzymol. 2020;641:373-399. doi:

    Insert

    E Coli tRNA Guanine Transglycosylase-His
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. Ki67 (LR)-mNeonGreen-IRESpuro2

    Plasmid

    #183743

    Purpose

    Expresses Ki67 (LR)-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    LR domain of Ki67

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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