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Bacterial Expression

29,744 results

Showing: 5851 - 5900 of 29744 results

  4. pXPR-SF61

    Plasmid
    #163916
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Qianzhu et al J Am Chem Soc. 2020 Oct 14;142(41):17277
    Insert
    SF5Phe tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    163916-plasmid-map-sequence-id-327914

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  5. pACDura3

    Plasmid
    #138711
    Purpose
    Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.
    Depositor
    Víctor de Lorenzo
    Article
    Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21
    Insert
    ura3 gene (URA3 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    optimized ura3 gene for expression in Escherichia…
    Promoter
    pEM7 promoter
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    138711-plasmid-map-sequence-id-311288

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  6. pECTO

    Plasmid
    #67683
    Purpose
    pMAD2 derivative designed for chromosomal gene ectopic complementation in Staphylococcus aureus
    Depositor
    Philippe Bouloc
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    67683-plasmid-map-sequence-id-176849

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  11. SPLICSFAST-ER-MT-P2A

    Plasmid
    #214892
    Purpose
    Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-ER-MT-P2A
    Use
    Tags
    none
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214892-plasmid-map-sequence-id-423478

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  17. pRC319

    Plasmid
    #61272
    Purpose
    Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61272-plasmid-map-sequence-id-99585

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  18. pTrcHisA-PPARalpha(aa101-468)

    Plasmid
    #13574
    Depositor
    Martin Scanlon
    Insert
    human peroxisome proliferator activated receptor alpha A/B domain deletion construct (aa101-468) (PPARA Human)
    Use
    Tags
    x6His N-terminal, enterokinase cleavage site
    Expression
    Bacterial
    Mutation
    deleted residues 1-100.
    Promoter
    Available since
    March 15, 2007
    Availability
    Academic Institutions and Nonprofits only
    13574-plasmid-map-sequence-id-142611

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  19. pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

    Plasmid
    #108693
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme
    Depositor
    Martin Reijns, Andrew Jackson
    Article
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A-D34A/D169A
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon, …
    Promoter
    tac
    Available since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    108693-plasmid-map-sequence-id-208987

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  20. SBC015866

    Plasmid
    #226280
    Purpose
    Expresses BsSfp, MsCAD, and SrCAR from trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.
    Depositor
    Rainer Breitling
    Article
    Hanko et al Microb Cell Fact. 2023 Nov 18;22(1):238.
    Inserts
    4'-phosphopantetheinyl transferase Sfp
    Cinnamyl alcohol dehydrogenase
    Carboxylic acid reductase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 25, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226280-plasmid-map-sequence-id-452181

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  24. pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

    Plasmid
    #90473
    Purpose
    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
    Depositor
    Hermann Gaub
    Insert
    TagGFP2-TEV-GGG-ELP(120nm)-Cys
    Use
    Tags
    Cysteine and Sortase-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90473-plasmid-map-sequence-id-191118

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  27. pENTR11 CA NFAT2

    Plasmid
    #11793
    Depositor
    Anjana Rao
    Insert
    NFAT2 (Nfatc1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Constitutively active: multiple serine to alanine…
    Promoter
    Available since
    Aug. 7, 2006
    Availability
    Academic Institutions and Nonprofits only

  33. pFEndoNA

    Plasmid
    #174724
    Purpose
    Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.
    Depositor
    Jukka Finne
    Article
    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149
    Insert
    GFP-endoNA fusion protein
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    174724-plasmid-map-sequence-id-343066

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  36. pSCrhaB2plus

    Plasmid
    #164226
    Purpose
    Broad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.
    Depositor
    Silvia Cardona
    Article
    Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Rhamnose-inducible
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164226-plasmid-map-sequence-id-325696

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  37. pAD-KPS12-Pman

    Plasmid
    #133832
    Purpose
    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.
    Depositor
    Oscar Kuipers
    Article
    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57
    Insert
    mKate(KPS12)
    Use
    E.coli/bacillus spp. shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pman
    Available since
    Nov. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    133832-plasmid-map-sequence-id-261722

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  39. pET_BGL2

    Plasmid
    #101911
    Purpose
    Expresses a β-glucosidase gene from Thermobaculum terrenum in E. coli under the control of the pET system.
    Depositor
    Steve Shih
    Article
    Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944
    Insert
    β-glucosidase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7+LacO
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    101911-plasmid-map-sequence-id-197171

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  42. pET28-Dnmt3a3L-sc27

    Plasmid
    #71827
    Purpose
    For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.
    Depositor
    Albert Jeltsch
    Article
    Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do
    Insert
    Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    71827-plasmid-map-sequence-id-254300

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  43. pET21-Flavidin

    Plasmid
    #89881
    Purpose
    Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling
    Depositor
    Mark Howarth
    Article
    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-
    Insert
    Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
    Use
    Tags
    6 glutamate tag (C terminal on insert)
    Expression
    Bacterial
    Mutation
    K80R, K121R, K132R, K134R
    Promoter
    Available since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89881-plasmid-map-sequence-id-177281

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  46. pQmod3E-GG

    Plasmid
    #191348
    Purpose
    Clostridium expression vector (pCB102 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites
    Depositor
    Andrew Tolonen
    Article
    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-
    Insert
    Plac-RFP (IPTG-inducible red fluorescent protein)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Plac
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    191348-plasmid-map-sequence-id-375365

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  48. SEPX1

    Plasmid
    #39048
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SEPX1 (MSRB1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    SeCys95-S
    Promoter
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 5851 - 5900 of 29744 results