34,148 results

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pPSU20-40-60-80

Plasmid

#173839

Purpose

Coexpresses 20, 40, 60 and 80 kD Penn State ladder proteins

Depositor

Song Tan

Publication

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACCBPv3

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSL1521 (pSPIN, pSC101* backbone)

Plasmid

#160729

Purpose

Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.

Depositor

Samuel H. Sternberg

Publication

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmal_c5x_RNAse_Inhib

Plasmid

#153314

Purpose

Bacterial expression of an RNase inhibitor

Depositor

Drew Endy, Philippa Marrack

Insert

RNAse Inhibitor

Tags

Maltose Binding Protein

Expression

Bacterial

Available since

June 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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N8his-GFPenhancer-GGGGS4-LaG16

Plasmid

#140442

Purpose

We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

Depositor

Motoyuki Hattori

Publication

Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

Insert

GFPenhancer-GGGGS4-LaG16

Tags

8 histidine tag

Expression

Bacterial

Promoter

T7 promotor

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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T4 Lysozyme WT*

Plasmid

#18111

Depositor

Brian Matthews

Insert

T4 Lysozyme (e Bacteriophage T4)

Expression

Bacterial

Mutation

No cysteines: C54T, C97A

Available since

Aug. 26, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLBH531_MBP-Cas14a1 expression

Plasmid

#112500

Purpose

Expresses MBP fused to Cas14a1

Depositor

Jennifer Doudna

Publication

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Promoter

T7

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pEGFPN1-human Dynamin 1aa K44A

Plasmid

#22197

Depositor

Pietro De Camilli

Publication

Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

Insert

human Dynamin 1aa K44A (DNM1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

K44A

Available since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pET28c-F30-2xdBroccoli

Plasmid

#66843

Purpose

Expresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cells

Depositor

Samie Jaffrey

Publication

Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Expression

Bacterial

Promoter

T7

Available since

July 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pNatB (pACYCduet-naa20-naa25)

Plasmid

#53613

Purpose

Allows expression of the fission yeast NatB complex - chloramphenicol marker

Depositor

Dan Mulvihill

Publication

Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

Inserts

naa20 (naa20 Fission Yeast)
naa25 (naa25 Fission Yeast)

Expression

Bacterial

Promoter

T7

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pET-15b-TEV-C9R

Plasmid

#118754

Purpose

TEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activity

Depositor

Yutaka Kuroda

Publication

Nautiyal et al N Biotechnol. 2018 May 25;42:77-84. doi:

Insert

Tobacco Etch Virus protease (NEWENTRY Synthetic)

Tags

C9R and His tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH5

Plasmid

#71714

Purpose

Membrane scaffold protein (MSP), helix 5 deletion

Depositor

Gerhard Wagner

Publication

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRSET Nucleolin

Plasmid

#13037

Depositor

Peter Newburger

Publication

Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

Insert

Nucleolin (NCL Human)

Tags

6xHis

Expression

Bacterial

Available since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pUC18R6KT-mini-Tn7T-Km

Plasmid

#64969

Purpose

mini-Tn7 base vector with transcriptional terminator and oriT

Depositor

Herbert Schweizer

Publication

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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pUC57-mitoTPNOX

Plasmid

#87854

Purpose

This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

Depositor

Vamsi Mootha

Publication

Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

Insert

mitoTPNOX

Tags

C-terminal FLAG tag with a linker and mitochondri…

Expression

Bacterial

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SP-Cas9

Plasmid

#62731

Purpose

Expresses SP-Cas9 protein in bacterial cells

Depositor

Niels Geijsen

Publication

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

SP-CAS9

Use

CRISPR

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pRsetB-his7-Perceval

Plasmid

#20336

Depositor

Gary Yellen

Publication

Berg et al Nat Methods. 2009 Feb;6(2):161-6. doi: 1

Insert

Perceval (fluorescent ATP:ADP sensor)

Tags

his(7)

Expression

Bacterial

Available since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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ilux pGEX(-)

Plasmid

#107879

Purpose

Bacterial expression of ilux for bioluminescence imaging

Depositor

Stefan Hell

Publication

Gregor et al Proc Natl Acad Sci U S A. 2018 Jan 30;11

Insert

ilux

Expression

Bacterial

Promoter

tac

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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HLTV-hTRIM21

Plasmid

#104973

Purpose

Purification of His-Lipoyl-hTRIM21 protein from bacteria

Depositor

Leo James

Publication

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Tripartite Motif containing 21 (TRIM21 Human)

Tags

His-Lipoyl

Expression

Bacterial

Promoter

T7

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pSF1389

Plasmid

#104962

Purpose

Bacterial expression plasmid of bdSENP1 protease

Depositor

Dirk Görlich

Publication

Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

Insert

SENP1

Tags

His14 tag and Tev protease cleavage site

Expression

Bacterial

Promoter

lacUV5

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pRsetB-PercevalHR

Plasmid

#49081

Purpose

Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Publication

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Tags

7xHis

Expression

Bacterial

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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