31,059 results
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Plasmid#171586PurposeBacterial expression of NLS-RfxCas13d-NLS-HADepositorInsertHis-MBP-NLS-RfxCas13d-NLS-HA
TagsHAExpressionBacterialAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_2A4D
Plasmid#234196PurposeHeterologous protein expression of ubiquitin-conjugating enzyme E2 variant 1 (UEV-1) (CROC-1) in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pG108-K - AhpC-Glow
Plasmid#185135PurposeBacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoterDepositorInsertsGlow gene
Bacteroides thetaiotaomicron ahpC promoter
UsePg106ExpressionBacterialAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE133
Plasmid#118487Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsYFP2
ExpressionBacterialAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
WP_010880027.1
Plasmid#194729PurposeLumazine synthase of the hyperthermophile Aquifex aeolicus bacteriumDepositorInsertnanoparticle-proteinA and His tag
TagsHis tagExpressionBacterialPromoterT7Available SinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE-600n
Plasmid#13537DepositorInsertFLIP-E WT
TagsECFP and VenusExpressionBacterialAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLPP6
Plasmid#209968PurposeContains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertionDepositorInsertPromoter Dropout (sfGFP)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTSlb-wt
Plasmid#60728PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…Available SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEC3006 (pSpy1C-PZn_ffluc)
Plasmid#218515Purposereplicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenesDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of Plac_mrfp cassettePromoterPZn (PczcD)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT21
Plasmid#89662Purposeexpression of doubleTAL staple protein dT21 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT21
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCCL-GKL1RC-Short-LEUg1mKateg2GKL2ORF10UCS
Plasmid#52345PurposeIntegration cassette to create p1-Short-Leu-mKate with p2 ORF10 UCS>mKateDepositorInsertmKate2
UseP1 integration vectorExpressionBacterialMutationchanged V2HPromoterpGKL2 ORF10 UCSAvailable SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-PIPK1 gamma 87
Plasmid#22300DepositorInserthuman phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)
TagsGFPExpressionBacterial and MammalianAvailable SinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT32
Plasmid#89667Purposeexpression of doubleTAL staple protein dT32 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT32
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pR562
Plasmid#207886PurposeCreation of Level 0 entry clonesDepositorInsertamilCP
ExpressionBacterialAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRE130
Plasmid#118484Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertTagBFP2
ExpressionBacterialAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT16
Plasmid#89659Purposeexpression of doubleTAL staple protein dT16 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT16
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-Spy
Plasmid#90102PurposeIPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.DepositorAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTmpKm
Plasmid#176227PurposeA template plasmid for amplification of the cI-hok-neo cassetteDepositorInsertcI of phage lambda, hok of the R1 plasmid, neo
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGex-Cav1
Plasmid#33364DepositorAvailable SinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 aSyn C141
Plasmid#108866PurposeExpresses human WT alpha synuclein with a C-terminal cysteineDepositorInsertalpha synuclein (SNCA Human)
ExpressionBacterialMutationC141 mutation to allow C-terminal maleimide dye l…PromoterT7Available SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRsetB-his7-Perceval
Plasmid#20336DepositorInsertPerceval (fluorescent ATP:ADP sensor)
Tagshis(7)ExpressionBacterialAvailable SinceMarch 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET22b-csgA
Plasmid#89581PurposeExpress csgA curli protein with GS linker and HistaqDepositorAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ centrin-mKate2
Plasmid#105941Purposein vitro transcription, zf centrin N-terminally tagged with mkate2DepositorInsertCentrin
TagsmKate2ExpressionBacterialAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBWB294
Plasmid#140636PurposepBAV1k-lacI-Trc-BCD9-mCherry. BCD RBS.DepositorInsertmCherry
ExpressionBacterialAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFloat.T7_v1
Plasmid#133070PurposeEntry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.DepositorTypeEmpty backboneUseEntry vectorTags3C-His6 and His6-3CExpressionBacterialPromoterT7Available SinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
1GFP-His-GFP-TEV-cs-ATG3
Plasmid#197472PurposeBacterial Expression of human ATG3DepositorAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyB-GFP
Plasmid#220190PurposesgRNA expression vector - pBG35 promoter with GFP in sgRNA siteDepositorInsertpBG35 promoter with GFP in sgRNA site
UseCRISPRExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG35Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
QUAS-0-T7-GFP_ColE1
Plasmid#171649PurposeQUAS directly upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-0-T7
TagsssrA degradation tag (DAS+4)ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-QF_p15A
Plasmid#171651PurposeExpresses QF activator protein in BL21(DE3) E. coli. Contains p15A origin of replication.DepositorInsertQF-T7 Stop
ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-5-T7-GFP_ColE1
Plasmid#171653PurposeQUAS five base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-5-T7
TagsssrA degradation tag (DAS+4)ExpressionBacterialAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-10-T7-GFP_ColE1
Plasmid#171654PurposeQUAS ten base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-10-T7
TagsssrA degradation tag (DAS+4)ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-15-T7-GFP_ColE1
Plasmid#171655PurposeQUAS fifteen base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-15-T7
TagsssrA degradation tag (DAS+4)ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lse_C2c2_WT
Plasmid#83486PurposeBacterial expression of WT L. seeligeri C2c2 CRISPR effectorDepositorInsertLse_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialAvailable SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
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KHK
Plasmid#25532PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28_6xHis-PARP1delBRCT
Plasmid#173938PurposeBacterial expression of PARP1 lacking BRCT domainDepositorInsertPARP1delBRCT (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 374-520; V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSG360zeo
Plasmid#27154DepositorTypeEmpty backboneUseMycobacterial ko vectorExpressionBacterialAvailable SinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCE38
Plasmid#174432PurposeC. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.DepositorInsertC. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
UseCRISPRExpressionBacterialAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCE41
Plasmid#174433PurposeC. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.DepositorInsertHYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
UseCRISPRExpressionBacterial and YeastAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT31
Plasmid#89666Purposeexpression of doubleTAL staple protein dT31 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT31
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only