31,059 results
-
Plasmid#136927PurposeDirected evolution of SpCas9DepositorInsertCas9(N)-NpuN
UseCRISPRExpressionBacterialMutationsee manuscriptAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21b-RBD-JA1
Plasmid#167126PurposeExpresses SARS-CoV-2 RBD in bacterial systemDepositorInsertSARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)
ExpressionBacterialMutationresidues (333 to 529 aa)PromoterT7Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-PA-Rac1
Plasmid#22007PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
Tags6X His and mVenusExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546), Rac1 starts at I4 and contain…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pYTRW26K_1Ti1
Plasmid#177292Purposegenomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and eYFPDepositorInsertPsacB-sacB
UseSynthetic Biology; Yeast expression, rrn genomic …ExpressionBacterialAvailable SinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDWJ26
Plasmid#207603PurposeGFP-AIA expressionDepositorInsertGFP-AIA
ExpressionBacterialAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pO_72
Plasmid#78235PurposeTranscripts the r_oligo part that includes the HIV reverse transcriptase binding site (HTBS)DepositorInsertr_oligo
ExpressionBacterialPromoterj23100Available SinceAug. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS mNotch1-IC (DM#270)
Plasmid#15079DepositorAvailable SinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCK351
Plasmid#129696PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120034435
ExpressionBacterialAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
DCX-RFP
Plasmid#32851DepositorAvailable SinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
WasCFP /pQE-30
Plasmid#79858PurposeCyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.DepositorInsertWasCFP
TagsHisTagExpressionBacterialMutationCyan fluorescent protein mCerulean with substitut…PromoterT5 promoter / lac operatorAvailable SinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b-GBP-MTn
Plasmid#154363PurposeBacterial expression of MTnDepositorInsertMTn
TagsHisExpressionBacterialAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mNectarine
Plasmid#21717PurposeBacterial expression of pH-sensitive monomeric mNectarine red fluorescent proteinDepositorInsertmNectarine fluorescent proteins
TagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…Available SinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
hDHFR
Plasmid#184707Purposebacterial expression of human DHFRDepositorAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a SUMO Nup53xl 1-320
Plasmid#85998Purposebacterial expression of his6 and SUMO tagged Xenopus laevis Nup53DepositorInsertXenopus laevis Nup53 (nup35.L Frog)
TagsSUMOExpressionBacterialMutationcodon optimzed for bacterial expressionPromoterT7Available SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSEVA228-pro4IUPi
Plasmid#122018PurposeExpresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.DepositorInsertsExpressionBacterialMutationCodon optimized for E. coliPromoterpro4Available SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-TAX1BP1
Plasmid#171423PurposeFor the purifcation of TAX1BP1 proteinDepositorInsertTAX1BP1 (TAX1BP1 Human)
ExpressionBacterialAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJB166
Plasmid#86998PurposeCRISPR/Cas9 in fission yeast using fluoride selectionDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-XI
Plasmid#157988PurposeExpresses XI in E.coli cellDepositorInsertxylose isomerase
ExpressionBacterialAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2D3
Plasmid#15784DepositorInsertUbiquitin conjugating enzyme UbE2D3 (UBE2D3 Human)
TagsHisExpressionBacterialMutationsee Depositor Comments belowAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
HA-mDyn2 pcDNA3
Plasmid#36264DepositorAvailable SinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp15
Plasmid#156481PurposeBacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tagDepositorInsertNsp15 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-Galectin-8 FL
Plasmid#68805PurposeExpress Galectin-8 FL protein in Bacterial cellsDepositorInsertGalectin-8 (LGALS8 Human)
TagsN-terminal 6-histidine-tagExpressionBacterialMutationNo mutationPromoterT7Available SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAJM.1642
Plasmid#108535PurposeCinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.DepositorInsertCinRAM + PCin-YFP
ExpressionBacterialMutationCinR: S72C V80I G114D G115D R137H K220R A224T *24…Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNDGG003
Plasmid#231316PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Tc-par-ELT4; GG assembly from pOGG010, pOGG012, pOGG014, pOGG042, and pOGG004, TcRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJT119b
Plasmid#50551PurposeExpresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoterDepositorInsertsCcaS
CcaR
sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
His-GST
Plasmid#215169PurposeEncodes the synthetic glutathionine S-transferase with N-terminal His tagDepositorInsertHis-GST
TagsHis-tagExpressionBacterialPromoterT7 lacAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, G175R)-Strep
Plasmid#196211PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
TagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationG175RAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, G189E)-Strep
Plasmid#196212PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
TagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationG189EAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB402-er-mCherry
Plasmid#186288PurposeBinary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)DepositorInserter-mCherry
ExpressionBacterialAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-BSA4-G-L11
Plasmid#127830PurposeProduction of recombinant human ribosomal protein L11DepositorAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA FLARE.sc Biosensor Q63L
Plasmid#12151DepositorInsertRhoA Biosensor Q63L (RHOA Human)
Tags6xHisExpressionBacterial, Insect, and Mamm…MutationRhoA Q63L (dominant-positive mutation)Available SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
p3035 GST-HPV-18 E6
Plasmid#10850DepositorAvailable SinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRL542
Plasmid#70282PurposeColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genesDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMBP-SpyCatcher
Plasmid#79053PurposeExpresses maltose binding protein (MBP) SpyCatcher fusionDepositorInsertMBP-SpyCatcher
Tags6xHis and Maltose binding proteinExpressionBacterialMutationDerived from pDEST14-SpyCatcher (Plasmid #35044)…PromoterT7Available SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PPP1R12A wt
Plasmid#31669DepositorAvailable SinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-NQO1
Plasmid#177140PurposeBacterial vector for expression of an N-terminal GST fusion of NQO1 with a TEV protease site located between the GST tag and NQO1DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBLADE_ONLY_C
Plasmid#168050PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.DepositorInsertBlue light-inducible AraC dimers in Escherichia coli
ExpressionBacterialPromoterJ23101**Available SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZa pBAD csgA CBDcex pLac Ara csgGEF
Plasmid#226378PurposePlasmid carries csgA and csgG,E,F genes of curli operonDepositorInsertcsgA and csgG,E,F genes of curli operon
ExpressionBacterialPromoterpBADAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET_MBP-RGG-RGG
Plasmid#124946PurposeExpression of MBP-RGG-RGGDepositorAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLG327
Plasmid#128797PurposeXylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.DepositorAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only