29,754 results

1. pKEW212-MBP-TEV-AcrVA4

   Plasmid

   #115658

   Purpose

   Expresses AcrVA4 in bacteria via T7 RNAP, for purification

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   AcrVA4
   

   Use

   Tags

   MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNAP

   Available since

   Oct. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX nsp1 CoV2

   Plasmid

   #175512

   Purpose

   For protein expression of WT nsp1 CoV2

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nsp1 CoV2
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pIC-Cre

   Plasmid

   #19131

   Depositor

   Klaus Rajewsky

   Article

   Gu et al Cell. 1993 Jun 18. 73(6):1155-64.

   Insert

   Cre recombinase (cre Bacteriophage P1)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pDD04neo pmyo2::gfp

   Plasmid

   #26393

   Depositor

   Denis Dupuy

   Article

   Giordano-Santini et al Nat Methods. 2010 Aug 22. ():.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Worm

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-51b-HaloTag11

   Plasmid

   #175519

   Purpose

   Expression of HaloTag11 in bacterial cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   HaloTag11
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TPH2

   Plasmid

   #73243

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TPH2 (TPH2 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pH6HTC_MANF-Halo

   Plasmid

   #175341

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   MANF (MANF Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. eGFP-CBP

   Plasmid

   #169211

   Purpose

   Expression of eGFP-CBP protein for chitin visualization

   Depositor

   James S Fraser

   Article

   Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

   Insert

   Chitinase A1 Chitin Binding Domain
   

   Use

   Tags

   6xHis, Mxe Gyr Intein, and eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-2T-Cdc42-Q61L

   Plasmid

   #12971

   Depositor

   Gary Bokoch

   Insert

   Cdc42 constitutively active (CDC42 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Q61L Constitutively active

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pNHEJ-Cas9-recX

    Plasmid

    #158715

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHelper-CBE-sgRNA

    Plasmid

    #221137

    Purpose

    Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    sgRNA-scaffold with Cas9 handle
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119(SpeI)

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCB-CBEv1

    Plasmid

    #221138

    Purpose

    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq-Ptac

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCOLA-Gent-yibDp-Erv1p

    Plasmid

    #202483

    Purpose

    Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCPP5099

    Plasmid

    #128714

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopK1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCPP5328

    Plasmid

    #128721

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopU1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCPP5306

    Plasmid

    #128712

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopH1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCPP5571

    Plasmid

    #128713

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopI1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCPP5539

    Plasmid

    #128728

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopAF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCPP5618

    Plasmid

    #128709

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopE1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCPP5617

    Plasmid

    #128706

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopB1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBiex-1 Myosin VI HMM_SNAP

    Plasmid

    #82716

    Purpose

    Expresses Myosin VI HMM with a C-terminal SNAP tag.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

    Insert

    Myosin VI
    

    Use

    Tags

    6xHis Tag, FLAG purification tag, and SNAP tag

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    T7 Promoter

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEB1-mGFPmut3

    Plasmid

    #103981

    Purpose

    Plasmid encoding mGFPmut3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mGFPmut3
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S65G, S72A, …

    Promoter

    proC

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJMP1187

    Plasmid

    #119256

    Purpose

    rfp "test" strain

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX-6P-1 Stu2 1-306

    Plasmid

    #38315

    Depositor

    Anthony Hyman

    Article

    Widlund et al Mol Biol Cell. 2012 Sep 19.

    Insert

    5' 918 bp from STU2 ORF (STU2 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. plRL118

    Plasmid

    #163632

    Purpose

    Renilla luciferase reporter plasmid; Giles integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mycobacterial Optimized Promoter (MOP)

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDSG318

    Plasmid

    #115595

    Purpose

    surface-displayed Ag2, used in composability experiments; medium copy

    Depositor

    Ingmar Riedel-Kruse

    Article

    Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

    Insert

    regulated adhesin construct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. cr3Ec

    Plasmid

    #184860

    Purpose

    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    Cas9, gam, bet, exo
    

    Use

    Tags

    Cas9 ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMT1002

    Plasmid

    #8621

    Depositor

    Robert Hartley

    Article

    Hartley et al Protein Peptide Letters 1996 3:225-231

    Insert

    barnase, barstar
    

    Use

    Tags

    as pMT416

    Expression

    Bacterial

    Mutation

    CI157 lambda repressor gene has two HindIII sites…

    Promoter

    Available since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

29. 6ASB (FBXL19)

    Plasmid

    #119954

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    FBXL19 (FBXL19 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 31-153

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pAC574

    Plasmid

    #127975

    Purpose

    AMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    hygB (hygromycin resistance marker)
    

    Use

    Fungal expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans trpC promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28a(+) 6xHis-TEV-IFIT1

    Plasmid

    #53557

    Purpose

    bacterial expression of IFIT1

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT1 (IFIT1 Human)
    

    Use

    Tags

    6xHIS and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 28, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pRSFDuet1-His-TEV-OTC_Ub

    Plasmid

    #172134

    Purpose

    Scaffold design for studying ubiquitin interacting proteins by cryo-EM.

    Depositor

    Shang-Te Danny Hsu

    Article

    Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

    Insert

    E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET-FLAG-SpCas9-HF1

    Plasmid

    #126770

    Purpose

    Expression of increased fidelity SpCas9-HF1 in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET-29b(+)_NSP4ss_SerM_NucA-myc

    Plasmid

    #218191

    Purpose

    Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Use

    Tags

    NSP4 signal secretion peptide and myc

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTGD

    Plasmid

    #88894

    Purpose

    Tandem gene duplication cassette for RecA mediated homologous recombination in E. coli

    Depositor

    Keith Tyo

    Article

    Love et al Methods Mol Biol. 2019;1927:37-45. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pOSIP-KH (KanR, HK022)

    Plasmid

    #45983

    Depositor

    Keith Shearwin, Drew Endy

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBWB294

    Plasmid

    #140636

    Purpose

    pBAV1k-lacI-Trc-BCD9-mCherry. BCD RBS.

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HC25 pComb3xss anti-Homer1 nanobody

    Plasmid

    #134718

    Purpose

    Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Homer1 (Mouse) recombinant llama nanobody HC25 (Homer1 L. glama (llama))
    

    Use

    Tags

    6xHis-HA

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEB2_mScarletI_mVenusNB-MrkHR113A

    Plasmid

    #182292

    Purpose

    expresses c-di-GMP mutant biosensor in E. coli

    Depositor

    Howard Berg

    Article

    Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

    Insert

    mVenusNB-MrkHR113A
    

    Use

    Tags

    mVenusNB internal fusion to MrkH with a R113A poi…

    Expression

    Bacterial

    Mutation

    changed arginine 113 to alanine, MrkH numbering …

    Promoter

    proC

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AKAP13

    Plasmid

    #73232

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AKAP13 (AKAP13 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 1976-2211

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

41. pET28a-SUMO-AsCas12f-2NLS

    Plasmid

    #171613

    Purpose

    NLS-AsCas12f1-NLS heterologous expression plasmid for RNP

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    NLS-AsCas12f1-NLS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCDFDuet-trAtCCD7-OsD27(pYL726 )

    Plasmid

    #178285

    Purpose

    Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from Arabidopsis

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Inserts

    OsD27
    trAtCCD7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET21a-trAtCCD8(pYL735)

    Plasmid

    #178286

    Purpose

    Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Insert

    trAtCCD8
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pXint129

    Plasmid

    #178208

    Purpose

    Encodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.

    Depositor

    Gregory Phillips

    Article

    Platt et al Plasmid. 2000 Jan . 43(1):12-23.

    Insert

    xis excisionase, int integrase (xis, lambdap32 Bacteriophage lambda)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacI

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pLITMUS-rpoN-cI-J23106-geneIII

    Plasmid

    #80843

    Purpose

    Phagemid (encodes M13 gene III and lambda cIopt).

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    M13 gene III
    Lambda cI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23106 and rpoN

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCSW21

    Plasmid

    #86836

    Purpose

    bacterial expression of anti-CRISPR protein AcrIIA4

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. pRSETb-ffDronpa

    Plasmid

    #78182

    Purpose

    Expresses ffDronpa E. coli strains

    Depositor

    Peter Dedecker

    Article

    Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

    Insert

    ffDronpa
    

    Use

    Tags

    6xHis-tag; Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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48. pPEPX-P3-sgRNAluc

    Plasmid

    #85590

    Purpose

    Integrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template for

    Depositor

    Jan-Willem Veening

    Article

    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

    Insert

    sgRNA targeting firefly luciferase encoding gene
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pGEX 4T1 ERbeta

    Plasmid

    #35563

    Depositor

    Harish Srinivas

    Article

    Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

    Insert

    Estrogen Receptor beta (ESR2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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50. pET28-Rsn2

    Plasmid

    #61686

    Purpose

    bacterial expression of ranaspumin 2 as a His-tagged fusion protein

    Depositor

    Brian Smith

    Article

    Mackenzie et al Biophys J. 2009 Jun 17;96(12):4984-92. d

    Insert

    Ranaspumin-2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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