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Bacterial Expression

29,754 results

Showing: 7051 - 7100 of 29754 results

  1. pSiM1

    Plasmid
    #199380
    Purpose
    Encodes dual cistronic membrane localized GCaMP6s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    GCaMP6s::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Available since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199380-plasmid-map-sequence-id-391037

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  7. pUC19-T7-SdB-T

    Plasmid
    #87307
    Purpose
    Expresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Stabilized dimeric Broccoli aptamer
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87307-plasmid-map-sequence-id-174665

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  9. pSaPIPLC His

    Plasmid
    #173810
    Purpose
    Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Mary F Roberts, Anne Gershenson
    Article
    Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87
    Insert
    Staphylococcus aureus plc
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173810-plasmid-map-sequence-id-341162

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  11. pHS1928

    Plasmid
    #205967
    Purpose
    JAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Insert
    Cas8-HNH all proteins, with His-tagged Cas8
    Use
    Tags
    14xHis on Cas8
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205967-plasmid-map-sequence-id-415395

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  12. pKB230

    Plasmid
    #170017
    Purpose
    Encodes LacI-controlled expression of surface-displayed protegrin-1 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    Protegrin-1
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    170017-plasmid-map-sequence-id-339960

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  20. pAM005

    Plasmid
    #170009
    Purpose
    Encodes PvirK-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    Superfolder GFP
    PhoP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1 and PvirK
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    170009-plasmid-map-sequence-id-335956

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  21. pET151-D-TOPO-PARP14-SΔK

    Plasmid
    #216658
    Purpose
    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.
    Depositor
    Herwig Schüler
    Article
    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.
    Insert
    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
    Use
    Tags
    6xHis-V5-TEV
    Expression
    Bacterial
    Mutation
    Deletion starting from Q1390 to D1448 and substit…
    Promoter
    T7
    Available since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    216658-plasmid-map-sequence-id-429340

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  22. pUC57_beta-globin-5'UTR_Nanoluc

    Plasmid
    #219989
    Purpose
    betaglobin-5'UTR-NanoLuc mRNA synthesis
    Depositor
    Simon Alberti
    Insert
    betaglobin-5'UTR-NanoLuc (HBB Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219989-plasmid-map-sequence-id-436835

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  23. pETTEV-SENP8

    Plasmid
    #196361
    Purpose
    protein expression of the His-tagged full length protein
    Depositor
    Stefan Knapp
    Insert
    SENP8/NEDP1
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196361-plasmid-map-sequence-id-386147

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  27. pBS mouse IRS-1

    Plasmid
    #11026
    Depositor
    Ronald Kahn
    Insert
    IRS-1 (Irs1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  28. pETM6-4F2-mCherry

    Plasmid
    #73421
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P4F2 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73421-plasmid-map-sequence-id-135321

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  29. pET15b/GgVcl 884-1066-ECFP

    Plasmid
    #46182
    Depositor
    Susan Craig
    Insert
    Vcl 884-1066 (VCL Chicken)
    Use
    Tags
    ECFP and His
    Expression
    Bacterial
    Mutation
    aa 884-1066 (tail domain)
    Promoter
    T7
    Available since
    July 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  30. pRSF-Ivy-his-hLYZ

    Plasmid
    #83920
    Purpose
    Co-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.
    Depositor
    Karl Griswold
    Article
    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do
    Inserts
    Ivy
    lysozyme (LYZ Human)
    Use
    Tags
    Ivy protein has C-terminal hisx6 tag
    Expression
    Bacterial
    Mutation
    Ivy leader sequence deleted and replaced.
    Promoter
    T7 and t7
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    83920-plasmid-map-sequence-id-160356

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  33. pAM5082

    Plasmid
    #85121
    Purpose
    PkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization exp
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    PkaiB-KaiC-ECFP
    Use
    Tags
    ECFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85121-plasmid-map-sequence-id-180106

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  37. pBsVchCAST-pyrE

    Plasmid
    #203813
    Purpose
    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli
    Depositor
    Sheng Yang
    Article
    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2
    Insert
    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203813-plasmid-map-sequence-id-403827

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  40. pEDF-PhdRS

    Plasmid
    #127445
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage
    Depositor
    Wenshe Liu
    Article
    Tharp et al Nat Commun. 2020 Mar 13;11(1):1392. doi:
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N346A-C348A mutations on PylRS
    Promoter
    araBAD
    Available since
    April 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    127445-plasmid-map-sequence-id-250213

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  41. H11-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

    Plasmid
    #180156
    Purpose
    tdTomato cre reporter with Hipp11 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    180156-plasmid-map-sequence-id-360161

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  46. pYES2.1-TRP1

    Plasmid
    #11007
    Depositor
    Francisco Malagon
    Insert
    TRP1 (TRP1 Budding Yeast)
    Use
    Tags
    V5 and his6
    Expression
    Bacterial and Yeast
    Mutation
    ORF-TRP1 without stop codon
    Promoter
    Available since
    Dec. 16, 2005
    Availability
    Academic Institutions and Nonprofits only
    11007-plasmid-map-sequence-id-6841

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  48. pNIC28-hKdm2b-CxxC-PHD

    Plasmid
    #49216
    Purpose
    Bacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.
    Depositor
    Rob Klose
    Insert
    Kdm2b (KDM2B Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    49216-plasmid-map-sequence-id-72771

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Showing: 7051 - 7100 of 29754 results