Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,671 results

Showing: 8151 - 8200 of 32671 results

  1. pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)

    Plasmid
    #128165
    Purpose
    UV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).
    Depositor
    Marcus Noyes
    Article
    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928
    Insert
    10 amino acid glycine/serine linker + 5alanines
    Use
    Synthetic Biology
    Tags
    FLAG and zif268
    Expression
    Bacterial
    Promoter
    UV5
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    128165-plasmid-map-sequence-id-267573

    Enlarge

  9. pCJ202

    Plasmid
    #162675
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    W159C, S238C; codon optimized for expression in E…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162675-plasmid-map-sequence-id-321470

    Enlarge

  11. pSW002-Pc-TorA(sp)-mTurquoise2

    Plasmid
    #111254
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter followed by E. coli TorA signal peptide in frame with mTurquoise2 (codon optimized for P. fluorescens); export to periplasm
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorA(sp)-mTurquoise2
    Expression
    Bacterial
    Mutation
    mTurquoise2 is codon optimized for expression in …
    Promoter
    Pc
    Available Since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111254-plasmid-map-sequence-id-215265

    Enlarge

  16. pBP-ORF-xylE

    Plasmid
    #112780
    Purpose
    Encodes catechol-2,3-dioxygenase, from Pseudomonas putida TOL pWW0.
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    xylE open reading frame
    Expression
    Bacterial
    Available Since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112780-plasmid-map-sequence-id-218368

    Enlarge

  25. mRuby-pBAD

    Plasmid
    #54763
    Purpose
    Localization: Protein Expression Vector , Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    6xHis and mRuby
    Expression
    Bacterial
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. pSUB7

    Plasmid
    #169698
    Purpose
    Template plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
    Tags
    6xHis tag
    Expression
    Bacterial
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169698-plasmid-map-sequence-id-334547

    Enlarge

  41. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

    Plasmid
    #181791
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181791-plasmid-map-sequence-id-354814

    Enlarge

  42. pAC-crRNA-Cm

    Plasmid
    #158711
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    eforCP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    TetO
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    158711-plasmid-map-sequence-id-325466

    Enlarge

  43. pET24a MDH_9CREN

    Plasmid
    #204243
    Purpose
    Bacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_9CREN
    Tags
    6X His
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204243-plasmid-map-sequence-id-413343

    Enlarge

  44. pET23b-Casp2-Delta Card

    Plasmid
    #11810
    Depositor
    Guy Salvesen
    Insert
    Caspase-2 Delta Card (CASP2 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Delta Card
    Available Since
    May 12, 2006
    Availability
    Academic Institutions and Nonprofits only
Showing: 8151 - 8200 of 32671 results