30,260 results

1. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

   Plasmid

   #216283

   Purpose

   Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SpyCatcher003-TEVs-SpyTag003DA
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRE162

   Plasmid

   #118510

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sGFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDusk

   Plasmid

   #43795

   Purpose

   Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

   Depositor

   Andreas Moeglich

   Article

   Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNCS YFP

   Plasmid

   #91759

   Purpose

   Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

   Insert

   Venus YFP (A206K)
   

   Use

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD_Kan-LacI

   Plasmid

   #79826

   Purpose

   Arabinose induced expression of Lac repressor

   Depositor

   Katja Arndt

   Article

   Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

   Insert

   LacI
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   araBp

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZa pBAD csgA CBDcex pLac Ara csgGEF

   Plasmid

   #226378

   Purpose

   Plasmid carries csgA and csgG,E,F genes of curli operon

   Depositor

   Urartu Seker

   Article

   Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

   Insert

   csgA and csgG,E,F genes of curli operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBtPIPLC His

   Plasmid

   #173808

   Purpose

   Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

   Depositor

   Anne Gershenson, Mary F Roberts

   Article

   He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

   Insert

   Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET51b-His-TEV-CLIP-tag

   Plasmid

   #167274

   Purpose

   Overexpression of CLIP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   CLIP-tag
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28_6xHis-PARP1-ART

   Plasmid

   #174810

   Purpose

   Bacterial expression of isolated PARP1 ART subdomain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-ART (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28_6xHis-PARP1-BRCT

    Plasmid

    #174807

    Purpose

    Bacterial expression of isolated PARP1 BRCT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-BRCT (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28_6xHis-PARP1-WGR

    Plasmid

    #174808

    Purpose

    Bacterial expression of isolated PARP1 WGR domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-WGR (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pReporter_4

    Plasmid

    #60565

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. FABP1A

    Plasmid

    #42344

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FABP1A (FABP1 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pAB061i3

    Plasmid

    #79230

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ; PlacIq lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppro1; PlacIq

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET12a-PAPI

    Plasmid

    #19961

    Depositor

    Jonathan Tait

    Article

    Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

    Insert

    Annexin V (ANXA5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLG024 (SIR2 + HerA)

    Plasmid

    #157902

    Purpose

    Expresses the SIR2 + HerA defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    SIR2 + HerA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mOrange-pBAD

    Plasmid

    #54751

    Purpose

    Localization: Protein Expression Vector , Excitation: 548, Emission: 562

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    6xHis and mOrange

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KAT2B

    Plasmid

    #39002

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KAT2B (KAT2B Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pGEX-5X-1-Full-Length-MePCE

    Plasmid

    #113539

    Purpose

    Produces an N-terminal GST-tagged full length MePCE protein.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    MePCE (MEPCE Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCR8-attL3-pegRNA-attL2

    Plasmid

    #213052

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    35S-CmYCLV-AtU6

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge