30,744 results

1. pBluescript II SK--POLQ

   Plasmid

   #64873

   Purpose

   cloning vector for human POLQ

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Insert

   POLQ (POLQ Human)
   

   Expression

   Bacterial

   Available Since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJBM4

   Plasmid

   #225291

   Purpose

   E. coli-Campylobacter shuttle vector; TetR

   Depositor

   Robert Moore

   Article

   McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

   Insert

   Tet(O) and its putative promoter
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX2T merlin 1-595

   Plasmid

   #11629

   Depositor

   Vijaya Ramesh

   Article

   Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

   Insert

   merlin (NF2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   May 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. OPEN-HLA-B*07:02-BirA

   Plasmid

   #239752

   Purpose

   E. coli expression of BirA tagged HLA-B*07:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-B*07:02 (HLA-B Human)
   

   Tags

   BirA substrate peptide

   Expression

   Bacterial

   Mutation

   G120C

   Promoter

   T7

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD-GreenPy1Highest

   Plasmid

   #239049

   Purpose

   Bacterial expression of highest affinity green fluorescent pyruvate biosensor GreenPy1Highest

   Depositor

   Robert Campbell

   Article

   Imai et al bioRxiv 2025.04.17.649293

   Insert

   GreenPy1Highest
   

   Expression

   Bacterial

   Available Since

   June 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-GreenPy1High

   Plasmid

   #239050

   Purpose

   Bacterial expression of high affinity green fluorescent pyruvate biosensor GreenPy1High

   Depositor

   Robert Campbell

   Article

   Imai et al bioRxiv 2025.04.17.649293

   Insert

   GreenPy1High
   

   Expression

   Bacterial

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-GreenPy1Lowest

   Plasmid

   #239052

   Purpose

   Bacterial expression of lowest affinity green fluorescent pyruvate biosensor GreenPy1Lowest

   Depositor

   Robert Campbell

   Article

   Imai et al bioRxiv 2025.04.17.649293

   Insert

   GreenPy1Lowest
   

   Expression

   Bacterial

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. OPEN-A*24:02

   Plasmid

   #235133

   Purpose

   E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-A*24:02 (HLA-A Human)
   

   Expression

   Bacterial

   Mutation

   G120C

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. OPEN-HLA-A*01:01

   Plasmid

   #235134

   Purpose

   E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-A*01:01 (HLA-A Human)
   

   Expression

   Bacterial

   Mutation

   G120C

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. OPEN-HLA-A*11:01-BirA

    Plasmid

    #235135

    Purpose

    E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN-HLA-A*11:01 (HLA-A Human)
    

    Tags

    BirA

    Expression

    Bacterial

    Mutation

    G120C

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJS119

    Plasmid

    #207592

    Purpose

    GFP-TonB deltaII expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-TonB deltaII
    

    Expression

    Bacterial

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJS104

    Plasmid

    #207589

    Purpose

    GFP-TolA deltaII expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-TolA deltaII
    

    Expression

    Bacterial

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJS70

    Plasmid

    #207586

    Purpose

    GFP-TolA H22A expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-TolA H22A
    

    Expression

    Bacterial

    Mutation

    H22A

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCA528-PPxY1-GS-WW3e

    Plasmid

    #166705

    Purpose

    expresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    AMOT PPxY1 fused to NEDD4L WW3
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUG-302

    Plasmid

    #104731

    Purpose

    Level 2 construct

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    HIS-Ubq_UBA1_HA-UBC11_EL3
    

    Tags

    HIS-Ubq, HA-E2

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYUB28b

    Plasmid

    #37277

    Depositor

    Ted Baker

    Article

    Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

    Type

    Empty backbone

    Use

    Mycobacteria shuttle vector

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD24-sfGFPx2

    Plasmid

    #51559

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

    Depositor

    Francisco Malagon, Sankar Adhya

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Tags

    GLESTCRHASLAVLADERRFSA and MARARA

    Expression

    Bacterial

    Mutation

    Contains additonal aa in the N and C terminus (in…

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ubiquitin (1-76; T66V, L67N)

    Plasmid

    #110755

    Purpose

    Bacterial expression of human Ubiquitin (1-76; T66V, L67N)

    Depositor

    David Komander

    Article

    Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

    Insert

    UBB (UBB Human)
    

    Expression

    Bacterial

    Mutation

    T66V, L67N

    Promoter

    T7

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. His6-EGFP-PH-PLCD1

    Plasmid

    #183675

    Purpose

    PtdIns(4,5)P2 biosensor for purification from bacteria

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLCD1 (PLCD1 Human)
    

    Tags

    His6-EGFP

    Expression

    Bacterial

    Mutation

    Amino acids 11-140

    Promoter

    T7

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSKB3.MPN348

    Plasmid

    #11437

    Depositor

    Sung-Hou Kim

    Article

    Chen et al Proteins. 2004 Sep 1. 56(4):839-43.

    Insert

    methenyltetrahydrofolate synthetase
    

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Available Since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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