Skip to main content
Addgene
Showing: 8751 - 8800 of 29620 results

  6. rrsB-RFP

    Plasmid
    #63838
    Purpose
    This plasmid is designed as a bacterial nutrient sensor
    Depositor
    Howard Salis
    Insert
    rrsB, mRFP1 and GFPmut3b
    Use
    Tags
    mRFP1
    Expression
    Bacterial
    Mutation
    Gene is transcriptionally fused to RFP
    Promoter
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    63838-plasmid-map-sequence-id-106561

    Enlarge

  9. pGEX-4T1 NLS-GFP

    Plasmid
    #129441
    Purpose
    bacterial expression of GFP
    Depositor
    Oskar Laur
    Insert
    GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129441-plasmid-map-sequence-id-256454

    Enlarge

  11. PTPRE

    Plasmid
    #38950
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRE (PTPRE Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. pCJ206

    Plasmid
    #162679
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    E226T; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162679-plasmid-map-sequence-id-321456

    Enlarge

  23. pBAD-hsGFP

    Plasmid
    #64911
    Purpose
    Encodes a Fluorescent Reporter for Hydrogen Sulfide in E. Coli
    Depositor
    Huiwang Ai
    Article
    Chen et al Biochemistry. 2014 Sep 23;53(37):5966-74
    Insert
    hsGFP
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    Chromophore tyrosine of the protein mutated to TA…
    Promoter
    araBAD
    Available since
    May 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    64911-plasmid-map-sequence-id-109660

    Enlarge

  24. pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]

    Plasmid
    #74102
    Purpose
    plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)
    Depositor
    Ben Szaro
    Article
    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:
    Inserts
    Xenopus laevis beta-tubulin gene promoter region and 5'UTR
    Green Lantern Green Fluorescent Protein
    Rabbit beta 1-globin gene 3'UTR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74102-plasmid-map-sequence-id-148113

    Enlarge

  25. pOPINO_C5

    Plasmid
    #171925
    Purpose
    Bacterial expression of nanobody VHH_C5, which binds to receptor binding domain of the spike protein of SARS-CoV-2
    Depositor
    Ray Owens
    Article
    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:
    Insert
    VHH_C5
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    171925-plasmid-map-sequence-id-338635

    Enlarge

  26. pTriEX-Antenna-GDI Cdc42

    Plasmid
    #101681
    Purpose
    "Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al
    Depositor
    Klaus Hahn
    Article
    Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/
    Insert
    Cdc42
    Use
    Tags
    6xHis and FRET Antenna (mCerulean-cpVenus)
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    CMV
    Available since
    March 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    101681-plasmid-map-sequence-id-194353

    Enlarge

  27. pJ601

    Plasmid
    #29509
    Depositor
    Jonathan Tait
    Insert
    annexin VI (ANXA6 Human)
    Use
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1.
    Promoter
    Available since
    April 14, 2011
    Availability
    Academic Institutions and Nonprofits only

  34. pOPINO_C1

    Plasmid
    #171924
    Purpose
    Bacterial expression of nanobody VHH_C1, which binds to receptor binding domain of the spike protein of SARS-CoV-2
    Depositor
    Ray Owens
    Article
    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:
    Insert
    VHH_C1
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    171924-plasmid-map-sequence-id-338631

    Enlarge

  38. pQCascade-IS1

    Plasmid
    #140624
    Purpose
    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS1. The crRNA-IS1 targets IS1 loci in Escherchia coli.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140624-plasmid-map-sequence-id-305995

    Enlarge

  42. CLK3

    Plasmid
    #38831
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CLK3 (CLK3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  44. pAL3

    Plasmid
    #114673
    Purpose
    T7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.
    Depositor
    William Studier
    Article
    Studier et al Curr Protoc Mol Biol. 2018 Oct;124(1):e6
    Type
    Empty backbone
    Use
    Tags
    optional Ser-Gly-6His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    114673-plasmid-map-sequence-id-254326

    Enlarge

  46. pCDF-NtXNtS1R1AtR2NtB2

    Plasmid
    #160881
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    rbcS-S1, rbcX, raf1, raf2, bsd2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-S1: T7, chaperones: T7
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160881-plasmid-map-sequence-id-318841

    Enlarge

  47. pGBW-m4092694

    Plasmid
    #153907
    Purpose
    Bacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153907-plasmid-map-sequence-id-307331

    Enlarge

Showing: 8751 - 8800 of 29620 results