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32,126 results

Showing: 7201 - 7240 of 32126 results

  1. pPLN-hFluc-WT-HA-GFP11-KDEL

    Plasmid
    #177722
    Purpose
    Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    hFluc
    Tags
    GFP11, HA, KDEL, and Prolactin Signal Sequence
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177722-plasmid-map-sequence-id-352603

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  3. pTPH413

    Plasmid
    #185728
    Purpose
    Bacterial PAM profiling of adenine base editor variants
    Depositor
    David Liu
    Article
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Inserts
    ecTadA(8e)-neNme2-C
    Nme2Cas9 sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…
    Available Since
    Sept. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185728-plasmid-map-sequence-id-364088

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  4. ASSA

    Plasmid
    #42368
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ASSA (ASS1 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. pEND-224_Cacnes_sfGFP

    Plasmid
    #225611
    Purpose
    Replicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    sfGFP
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225611-plasmid-map-sequence-id-451364

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  17. BRD7 (BRD7A-c005)

    Plasmid
    #98245
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD7A (BRD7 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00439:Bromodomain 136-223
    Promoter
    T7
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98245-plasmid-map-sequence-id-193423

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  18. pelB-MBP-BB0238-ss

    Plasmid
    #137046
    Purpose
    E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature BB0238: lacks the BB0238 signal peptide
    Promoter
    T7lac
    Available Since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    137046-plasmid-map-sequence-id-292071

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  20. pOGG012

    Plasmid
    #113986
    Purpose
    Vector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectors
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Partition genes (parABCDE)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113986-plasmid-map-sequence-id-223365

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  21. pTR47m4

    Plasmid
    #104068
    Purpose
    Luciferase Reporter for Formaldehyde Sensing
    Depositor
    David Liu
    Article
    Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.
    Inserts
    xluxAB
    frmR
    frm Promoter m4
    Use
    Luciferase and Synthetic Biology
    Expression
    Bacterial
    Mutation
    23r and 25th nucleotides in sequence mutated from…
    Available Since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    104068-plasmid-map-sequence-id-205132

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  23. pJCX3-ACTG2_R257C

    Plasmid
    #228911
    Purpose
    Expresses mutant R257C smooth muscle actin gamma-2 in human cells
    Depositor
    Roberto Dominguez
    Insert
    Actin gamma 2, smooth muscle (ACTG2 Human)
    Tags
    HIS-FLAG-TEV
    Expression
    Bacterial and Mammalian
    Mutation
    R257C
    Promoter
    CMV
    Available Since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    228911-plasmid-map-sequence-id-457665

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  24. pJCX3-ACTG2_WT

    Plasmid
    #228910
    Purpose
    Expresses wildtype smooth muscle actin gamma-2 in human cells
    Depositor
    Roberto Dominguez
    Insert
    Actin gamma 2, smooth muscle (ACTG2 Human)
    Tags
    HIS-FLAG-TEV
    Expression
    Bacterial and Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available Since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    228910-plasmid-map-sequence-id-457664

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  26. pFGL815

    Plasmid
    #52322
    Purpose
    A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.
    Depositor
    Naweed Naqvi
    Article
    Yang et al Fungal Genet Biol. 2014 Apr 24. pii: S10
    Type
    Empty backbone
    Use
    Agrobacterium
    Expression
    Bacterial
    Available Since
    July 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52322-plasmid-map-sequence-id-81025

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  31. pTJV1Sc-rpoB

    Plasmid
    #166978
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Inserts
    Ec86 Retron (ECD_00831 )
    lambda bet
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23101 and VanCC
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166978-plasmid-map-sequence-id-331328

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  33. pTXBI-mClover-YTH domain

    Plasmid
    #177123
    Purpose
    Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Article
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTH domain) (YTHDC1 Human)
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    YTH domain only (IDR1 and IDR2 deletion)
    Promoter
    T7
    Available Since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    177123-plasmid-map-sequence-id-348690

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  35. pET28GST-LIC

    Plasmid
    #26101
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6XHis, GST, and Thrombin CLeavage Site
    Expression
    Bacterial
    Available Since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26101-plasmid-map-sequence-id-142370

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  36. p6187

    Plasmid
    #198158
    Purpose
    bacterial expression of sifA::HaloTag fusion, mutant form
    Depositor
    Michael Hensel
    Insert
    PsifA sifA delta331-336::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Available Since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    198158-plasmid-map-sequence-id-386978

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Showing: 7201 - 7240 of 32126 results