32,671 results
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Plasmid#80773PurposeCloning vector compatible with ColE1 plasmids with medium copy numberDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only
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pET21-pelB-LaG-41
Plasmid#172757PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-41
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP12049
Plasmid#222355PurposeTn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.DepositorInsertsfGFP
ExpressionBacterialPromoterPabstBRAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBBR6k-GFPuv
Plasmid#106384PurposeIPTG-inducible LlacO-1 promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
ExpressionBacterialPromoterLlacO-1Available SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSecUAG_UTu1D
Plasmid#233867PurposeExpresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistantDepositorInsertsallo-tRNA UTu1D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
ExpressionBacterialMutationCysteine 32 switched to Selenocysteine (UAG) and …PromoterEM7, araBAD, and native As SelDAvailable SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB117
Plasmid#228481Purpose"Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)DepositorTypeEmpty backboneTagsGST (glutathione S-transferase)ExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET41c-PhTET2
Plasmid#182428PurposeExpresses P. horikoshii tetrahedral aminopeptidase 2 (TET2) in E. coliDepositorInsertTetrahedral aminopeptidase TET2 from P. hirokoshii
ExpressionBacterialAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Mms2
Plasmid#18893DepositorAvailable SinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
JMC044-B12
Plasmid#58752Purposebacterial expression of PDB 4NW3DepositorInsertPDB:4NW3 (KMT2A Human)
TagsHisExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available SinceAug. 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYC1876
Plasmid#158722PurposeHelper plasmid pYC1640 for C-terminal tagging with degradation tag ssrADepositorInsertssrA
UseCRISPRExpressionBacterialAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Tipin-His
Plasmid#23122DepositorAvailable SinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CAPZ
Plasmid#69948PurposeSimultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZDepositorTagsHis 6 and SNAPExpressionBacterialAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-dNmeCas9
Plasmid#71475Purposebacterial pMCSG7 expression vector expressing catalytically inactive Nme cas9 with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-Inactive-NmeCas9
Tags6HisExpressionBacterialMutationD16A H588AAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pBR322-Pdp1_NTD-ZFD_R
Plasmid#198277PurposeFor loading R half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Right-G1397-C_DddA); also contains PVC regulatory genesDepositorInsertPdp1_NTD-ZFD_R
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
KIF3A560GFP
Plasmid#129769PurposeExpresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP taggedDepositorInsertKIF3A (Kif3a Mouse)
TagsGFP and His6ExpressionBacterialMutationTruncation at 359, fused to kinesin-1 starting Al…Available SinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKEK3228
Plasmid#234090PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertaeBlue
ExpressionBacterialPromoterpJ23100-BsaIAvailable SinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_2EYI
Plasmid#234173PurposeHeterologous protein expression of alpha-actinin-1 in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
SEC14L3
Plasmid#73240PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
SEC14L4
Plasmid#73233PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28C-tCorn
Plasmid#124588PurposeBacterial Expression of tRNA-Corn aptamerDepositorInserttRNA-Corn
ExpressionBacterialPromoterlacIAvailable SinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbB5a-GFP
Plasmid#35352DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialPromoterlacUV5Available SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#15
Plasmid#153523PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#15DepositorInsertSb#15
ExpressionBacterialPromoterpBADAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSY6
Plasmid#90482PurposeTargeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicumDepositorInsertgroup II intron and ItrA
ExpressionBacterialAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCST-mAvicFP1
Plasmid#129509PurposeExpresses mAvicFP1 constitutively in E. coli (most strains)DepositorInsertmAvicFP1
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGV3340
Plasmid#170278PurposeExpression of mutant HIV-2 RT p51 reverse transcriptaseDepositorInsertHIV-2 RT p51 (mutant)
TagsHexahistidineExpressionBacterialPromoterT7-lacAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-rnc-sfGFP
Plasmid#51560PurposeGene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).DepositorInsertrnc (rnc E. coli)
TagsGLESTCRHASLAVLADERRFSA and superfolder GFPExpressionBacterialMutationContains additonal aa at the end of the sfGFP in …Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAB078d3
Plasmid#79203PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1); Ppro1Available SinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFosill-3
Plasmid#89697Purposeto make Fosmids that can be converted into Illumina sequencable librariesDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMM585
Plasmid#68884PurposeIPTG-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertsNanoLuc
LacI
ExpressionBacterialAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22b_pScaC-SNAP_6xHis
Plasmid#228835PurposeE. coli expression of the SNAP-tagged ScaC passenger domainDepositorInsertScaC
TagsSNAPtag-TEV-6xHisExpressionBacterialMutationaa 33-223Available SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
MT1X
Plasmid#105709PurposeBacterial expression plasmids for production of recombinant metallothionein 1xDepositorAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT1F
Plasmid#105703PurposeBacterial expression plasmids for production of recombinant metallothionein 1fDepositorAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLP-pR-himar-apraR
Plasmid#199996PurposeLanding Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donorDepositorInsertLanding Pads
ExpressionBacterialAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-R-GECO1.2
Plasmid#113937PurposeBacterial expression of red fluorescent Ca2+ sensor R-GECO1.2DepositorInsertR-GECO1.2
Tags6xHisExpressionBacterialAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-pBAD
Plasmid#54539PurposeLocalization: Protein Expression Vector , Excitation: 555, Emission: 584DepositorTypeEmpty backboneTags6xHis and mTagRFPExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21-pelB-LaG-42
Plasmid#172758PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-42
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKD077
Plasmid#136473PurposeSSB-mTur2 IDL fusion expression plasmidDepositorInsertSSB-mTur2 (ssb E. coli)
TagsmTurquoise2ExpressionBacterialMutationmTurquoise2 inserted between Phe148 and Ser149PromoterT7 promoterAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
ptRNAfMet-A37
Plasmid#196657PurposePlasmid for overexpression of native initiator tRNA (A37)DepositorInserttRNAfMet-A37 expression cassette
ExpressionBacterialPromotermetY native promoterAvailable SinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PPP1R1A
Plasmid#31331DepositorAvailable SinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109As(RS-te-AAV)
Plasmid#123156PurposeFor TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-tDepositorInsertJ109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-260n
Plasmid#13552DepositorInsertFLIPPi WT
TagsECFP and EYFPExpressionBacterialAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
RTA
Plasmid#64029PurposeExpression of Ricin A Chain (RTA) in E. coliDepositorInsertRicin A Chain
TagsnoneExpressionBacterialAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)
Plasmid#63139PurposeExpression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)DepositorTagsGSTExpressionBacterialMutationResidues 1-109 and Residues 1-159PromotertacAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET His6 TEV cloning vector with BioBrick polycistronic restriction sites (9B)
Plasmid#48284DepositorTypeEmpty backboneTagsHis6ExpressionBacterialAvailable SinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBZCas13b-HEPN
Plasmid#89901PurposeBacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.DepositorInsertCas13b
ExpressionBacterialMutationR116A/H121A/R1177A/H1182APromoterLacAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
GSG2
Plasmid#38915PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFLxR4
Plasmid#167517PurposeBroad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Carbenicillin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLuxBAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-10
Plasmid#172743PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-10
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
PTPRB
Plasmid#39165PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only