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31,059 results

Showing: 9201 - 9250 of 31059 results

  3. pASPIre1

    Plasmid
    #154842
    Purpose
    Derivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sites
    Depositor
    Markus Jeschek
    Article
    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:
    Insert
    Bxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
    Expression
    Bacterial
    Promoter
    Prha
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    154842-plasmid-map-sequence-id-307626

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  5. PARP3A

    Plasmid
    #42380
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PARP3A (PARP3 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pPICZa-STE2-1D4

    Plasmid
    #133436
    Purpose
    Mutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Expression
    Bacterial
    Mutation
    C59S/C252A
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133436-plasmid-map-sequence-id-267728

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  8. p28BIOH-LIC

    Plasmid
    #62352
    Purpose
    E. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and AviTag
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62352-plasmid-map-sequence-id-102272

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  13. RAB3B (3DZ8)

    Plasmid
    #32879
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB3B (RAB3B Human)
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 18-190 only
    Available Since
    Jan. 11, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pBT3007

    Plasmid
    #122538
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a early endosome localized gene (rab-5) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::rab-5 (rab-5 Nematode, Synthetic)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122538-plasmid-map-sequence-id-238129

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  16. pET-His6-Sumo-MBP-CfaN

    Plasmid
    #126016
    Purpose
    Bacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)
    Depositor
    Thomas Muir
    Insert
    His5-Sumo-MBP-CfaN
    Tags
    5xHis-SUMO
    Expression
    Bacterial
    Available Since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    126016-plasmid-map-sequence-id-248770

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  18. pTYB11-PTBP1-RRM2CCtoSS

    Plasmid
    #89154
    Purpose
    recombinant expression of PTBP1-RRM2 C250S,C251S in e.coli
    Depositor
    Frédéric Allain
    Article
    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n
    Insert
    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
    Tags
    Intein and chitin binding domain
    Expression
    Bacterial
    Mutation
    mutations: Cys 250 to Ser, Cys 251 to Ser
    Promoter
    T7
    Available Since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89154-plasmid-map-sequence-id-175723

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  21. PTPN4

    Plasmid
    #38828
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN4 (Ptpn4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  22. T4 Lysozyme S38D L99A M102Q N144D

    Plasmid
    #18477
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Expression
    Bacterial
    Mutation
    S38D L99A M102Q N144D
    Available Since
    Sept. 15, 2008
    Availability
    Academic Institutions and Nonprofits only

  23. pBiex PKC - Peptide 8 GFP RFP

    Plasmid
    #83564
    Purpose
    Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available Since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83564-plasmid-map-sequence-id-166406

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  24. pETM33_N_NTD

    Plasmid
    #156483
    Purpose
    Bacterial expression of Sars-CoV2 N_NTD protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    N_NTD (N Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156483-plasmid-map-sequence-id-307745

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  25. pKB233

    Plasmid
    #170040
    Purpose
    Encodes PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Insert
    mNeonGreen
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PvirK
    Available Since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170040-plasmid-map-sequence-id-336782

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  30. GARS_pNIC-Bio3

    Plasmid
    #153049
    Purpose
    Expression construct for producing Avi tagged Aspartyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    GARS (GARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153049-plasmid-map-sequence-id-304463

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  31. SLX4

    Plasmid
    #73242
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SLX4 (SLX4 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73242-plasmid-map-sequence-id-136207

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  38. pOGG010

    Plasmid
    #113984
    Purpose
    Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    RK2 (IncP)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113984-plasmid-map-sequence-id-223395

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  39. 4H4H

    Plasmid
    #51314
    Depositor
    Cheryl Arrowsmith
    Insert
    4H4H (EHMT1 4H4H, Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available Since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. KSP_K560GFP

    Plasmid
    #129770
    Purpose
    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 367, fused to kinesin-1 starting Al…
    Available Since
    Nov. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    129770-plasmid-map-sequence-id-263613

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  46. MYST1

    Plasmid
    #25180
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MYST1_01 (KAT8 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. mouse ptc probe M2-3

    Plasmid
    #58701
    Purpose
    makes mouse ptc probe for in situ hybridization
    Depositor
    Matthew Scott
    Insert
    ptc (Ptch1 Mouse)
    Use
    In situ probe
    Expression
    Bacterial
    Mutation
    only EcoRI fragment (5' end)
    Promoter
    T7
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  48. AKAP13

    Plasmid
    #73232
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AKAP13 (AKAP13 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 1976-2211
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 9201 - 9250 of 31059 results