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Showing: 10251 - 10300 of 29646 results

  5. NAT2

    Plasmid
    #25182
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NAT2_07 (NAT2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. sCFP in pBSDONR P4r-P2

    Plasmid
    #121530
    Purpose
    For making C-terminal sCFP fusions using multisite Gateway cloning
    Depositor
    Roger Innes
    Insert
    sCFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    121530-plasmid-map-sequence-id-234035

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  12. pDule-IBBN (G2)

    Plasmid
    #85500
    Purpose
    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-
    Insert
    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32G L65E F108W Q109M D158S R162K
    Promoter
    lpp (constitutive)
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85500-plasmid-map-sequence-id-169863

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  13. pDB036a

    Plasmid
    #99215
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK and PproD
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99215-plasmid-map-sequence-id-192376

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  14. NADK

    Plasmid
    #28268
    Depositor
    Cheryl Arrowsmith
    Insert
    NADK:HPC0AC-F12:C216828 (NADK Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    contains residues 68-426.
    Promoter
    Available since
    Feb. 10, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pETM41-EcPPK

    Plasmid
    #38334
    Depositor
    Florian Freimoser
    Insert
    E. coli polyphosphate kinase (ppk Escherichia coli)
    Use
    Tags
    6xHIS, MBP, and TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 10, 2012
    Availability
    Academic Institutions and Nonprofits only

  17. pMal-mRuby3

    Plasmid
    #209348
    Purpose
    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal mRuby3-StepTagII
    Depositor
    Lucia Strader
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    209348-plasmid-map-sequence-id-419546

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  18. pET-sCS4-2T

    Plasmid
    #28250
    Depositor
    Yawen Bai
    Article
    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.
    Insert
    single chain Cse4-Scm3-H4 with 2 Thrombin site
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    A thrombin cutting site between Cse4 and Scm3. An…
    Promoter
    Available since
    July 12, 2011
    Availability
    Academic Institutions and Nonprofits only

  21. pOPINO_F2

    Plasmid
    #171926
    Purpose
    Bacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2
    Depositor
    Ray Owens
    Article
    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:
    Insert
    VHH_F2
    Use
    Tags
    His6
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    Available since
    Sept. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    171926-plasmid-map-sequence-id-338638

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  26. PARP12A

    Plasmid
    #42383
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PARP12A (PARP12 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  27. pJEC581

    Plasmid
    #174369
    Purpose
    RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.
    Depositor
    James Chappell
    Article
    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793
    Insert
    RFP with PAM rich sequence upstream promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23117
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174369-plasmid-map-sequence-id-345702

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  31. DLC1A

    Plasmid
    #25333
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DLC1:S1074-L1283 (DLC1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids S1074-L1283
    Promoter
    Available since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  32. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

    Plasmid
    #169168
    Purpose
    Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.
    Depositor
    Sascha Martens
    Article
    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017
    Insert
    MAP1LC3B (MAP1LC3B Human)
    Use
    Tags
    6X Histidine Tag, TEV cleavage site, mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac promoter
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169168-plasmid-map-sequence-id-332048

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  33. p405CYC1

    Plasmid
    #15967
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    p405CYC1 (CYC1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    CYC1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15967-plasmid-map-sequence-id-8452

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  37. AHCYL1A

    Plasmid
    #42465
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AHCYL1A (AHCYL1 Human)
    Use
    Tags
    His6-StrepII-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  47. pRSF-G1mCNPRS

    Plasmid
    #174718
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi
    Insert
    tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    174718-plasmid-map-sequence-id-346623

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  49. pNCST-AvicFP4

    Plasmid
    #129507
    Purpose
    Expresses AvicFP4 constitutively in E. coli (most strains)
    Depositor
    Nathan Shaner
    Article
    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d
    Insert
    AvicFP4
    Use
    Tags
    Expression
    Bacterial
    Mutation
    One of two variants of AvicFP4 tested with essent…
    Promoter
    synthetic constitutive (stationary phase) promote…
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    129507-plasmid-map-sequence-id-271859

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  50. pLL1270

    Plasmid
    #129281
    Purpose
    The vector used for enzyme expression in Thermoanaerobacterium. thermosaccharolyticum throughout this study. Contains the CBP promoter from Clostridium thermocellum
    Depositor
    Lee Lynd
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Clostridium thermocellum DSM1313 Cellobiose Phosp…
    Available since
    March 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    129281-plasmid-map-sequence-id-260567

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Showing: 10251 - 10300 of 29646 results